scholarly journals Molecular detection of feline arthropod-borne pathogens in cats in Cuiabá, state of Mato Grosso, central-western region of Brazil

2013 ◽  
Vol 22 (3) ◽  
pp. 385-390 ◽  
Author(s):  
Natasha Gandolfi Miceli ◽  
Fernando Antonio Gavioli ◽  
Luiz Ricardo Gonçalves ◽  
Marcos Rogério André ◽  
Valéria Régia Franco Sousa ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Western Region ◽  
Mato Grosso ◽  
Animal Shelters ◽  
Blood Samples ◽  
Cytauxzoon Felis ◽  
The City ◽  
Veterinary Hospital

Hemotrophic mycoplasmas (hemoplasmas), Bartonellasp., Hepatozoon sp. and Cytauxzoon felis are prominent pathogens that circulate between cats and invertebrate hosts. The present study aimed to detect the presence of DNA from hemoplasmas,Bartonella sp., Hepatozoon sp. andCytauxzoon felis, and then confirm it by means of sequencing, in blood samples from cats in Cuiabá, MT, Brazil. From February 2009 to February 2011, blood samples with added EDTA were collected from 163 cats that were being housed in four different animal shelters in the city of Cuiabá, state of Mato Grosso, Brazil and from 15 cats that were admitted to the veterinary hospital of the Federal University of Mato Grosso (UFMT). Out of the 178 cats sampled, 15 (8.4%) were positive for hemoplasmas: four (2.2%) forMycoplasma haemofelis, 12 (6.7%) for ‘Candidatus M. haemominutum’ and one (0.5%) for ‘Candidatus M. turicensis’. One cat (0.5%), a patient that was attended at the veterinary hospital, was coinfected with M. haemofelis, ‘Candidatus M. haemominutum’ and ‘Candidatus M. turicensis’, based on sequencing confirmation. Four cats were positive for Bartonella spp.: three (1.7%) for B. henselae and one (0.5%) for B. clarridgeiae. None of the animals showedCytauxzoon sp. or Hepatozoon sp. DNA in their blood samples. This study showed that cats housed in animal shelters in the city of Cuiabá, state of Mato Grosso, are exposed to hemoplasmas andBartonella species.

scholarly journals Natural hemoplasma infection of cats in Cuiaba, Mato Grosso, Brazil

2018 ◽  
Vol 39 (2) ◽  
pp. 875 ◽  
Author(s):  
Herica Makino ◽  
Daphine Ariadne Jesus de Paula ◽  
Valéria Regia Franco Sousa ◽  
Adriane Jorge Mendonça ◽  
Valéria Dutra ◽  
...  
Keyword(s):  
Blood Count ◽  
Clinical Care ◽  
Mato Grosso ◽  
Blood Samples ◽  
Factors Associated ◽  
Mixed Breed ◽  
The City ◽  
Veterinary Hospital ◽  
Candidatus Mycoplasma Turicensis

The aim of this research was to investigate natural hemoplasma infection in cats treated at the Veterinary Hospital of the Federal University of Mato Grosso, and the factors associated with infection. Blood samples from 151 cats of different sexes, breeds, and ages were analyzed by PCR and blood count. The overall occurrence of hemoplasma was 25.8%. Mycoplasma haemofelis (Mhf), ‘Candidatus Mycoplasma haemominutum (CMhm)’, and ‘Candidatus Mycoplasma turicensis’ (CMt) were observed in 15.2%, 14.6% and 2.6% of cats, respectively. In 6.6 % of cases, co-infection was observed. Male felines or mixed breed cats were associated with infection by CMhm (P = 0.02 and 0.04, respectively). The data obtained demonstrated an occurrence of 25.8% for hemoplasma infection in felines coming from clinical care in the city of Cuiabá, where males were at higher risk of acquiring the infection by these agents, in addition to a higher risk for CMhm in felines with no specific breed.

scholarly journals Molecular detection of Hepatozoon canis and Babesia canis vogeli in domestic dogs from Cuiabá, Brazil

2011 ◽  
Vol 20 (3) ◽  
pp. 253-255 ◽  
Author(s):  
Mariana Granziera Spolidorio ◽  
Mariana de Medeiros Torres ◽  
Wilma Neres da Silva Campos ◽  
Andréia Lima Tomé Melo ◽  
Michelle Igarashi ◽  
...  
Keyword(s):  
18S Rrna ◽  
Molecular Detection ◽  
Domestic Dogs ◽  
Hepatozoon Canis ◽  
Babesia Canis ◽  
Mato Grosso ◽  
First Time ◽  
The City ◽  
Babesia Spp

The objective of this study was to report for the first time infection by Hepatozoon spp. and Babesia spp. in 10 dogs from the city of Cuiabá, State of Mato Grosso, central-western Brazil. A pair of primers that amplifies a 574 bp fragment of the 18S rRNA of Hepatozoon spp., and a pair of primers that amplifies a 551 bp fragment of the gene 18S rRNA for Babesia spp. were used. Six dogs were positive for Babesia spp., and 9 were positive for Hepatozoon spp. Co-infection of Babesia spp. and Hepatozoon spp. was seen in 5 dogs. Sequenced samples revealed 100% identity with B. canis vogeli, and H. canis. This is the first molecular detection of H. canis in domestic dogs from Cuiabá. Additionally, it is described for the first time the presence of B. canis vogeli circulating among dogs in Cuiabá.

scholarly journals Prevalence study on Trypanosoma vivax in dairy cattle in the Western region on the State of Paraná, Brazil

2018 ◽  
Vol 39 (1) ◽  
pp. 425
Author(s):  
Alessandra Snak ◽  
Arielle Aparecida Lara ◽  
Felipe Gustavo Garcia ◽  
Euclides Mario Pieri ◽  
Júlia Angélica Gonçalves da Silveira ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Buffy Coat ◽  
The State ◽  
Western Region ◽  
Trypanosoma Vivax ◽  
Serum Samples ◽  
Mato Grosso ◽  
Blood Samples ◽  
Negative Results ◽  
The Western Region

In Brazil, Trypanosoma vivax is present in several states. The disease is endemic in Pantanal and Minas Gerais. In Paraná there is still no report of the parasite, but due to the state borders with Mato Grosso do Sul, São Paulo, Paraguay and Argentina, it is believed that the protozoan circulates in the region without diagnosis. The objective of this study was to investigate the prevalence of T. vivax in dairy cattle in the western region of Paraná. For this purpose, 600 blood samples and 400 serum samples were collected from dairy cattle, distributed in 60 and 40 properties, respectively. While buffy coat smears were performed on blood samples, serum samples were used in Indirect Immunofluorescence Reaction. All samples, in both techniques, presented negative results for T. vivax. These results indicate that the studied hemoprotozoan is not circulating among the cattle in the western region of Paraná. However, future work evolving beef cattle must be carried out and preventive measures should be adopted in order to avoid the entry of the parasite in the State of Paraná.

scholarly journals Frequency of dogs with anti Leishmania Chagasi antibodies by immunochromatographic assay (DPP) in the city of Sinop, Mato Grosso, Brazil

2020 ◽  
Vol 13 (11) ◽  
pp. 97
Author(s):  
L. M. J. Lopes ◽  
M. V. Bonfim ◽  
F. S. C. Santi ◽  
T. R. Berndt ◽  
J. Bartolomei Neto ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Public Health Problem ◽  
Leishmania Chagasi ◽  
Mato Grosso ◽  
Wide Geographical Distribution ◽  
Health Agencies ◽  
Serious Disease ◽  
City State ◽  
The City ◽  
Veterinary Hospital

Dogs are considered the main reservoir for visceral leishmaniasis infection in humans, a serious disease that is a relevant public health problem due to its wide geographical distribution. To date, there haven’t been notification of Canine Visceral Leishmaniasis (CVL) in official health agencies of Sinop city, state of Mato Grosso. CVL diagnosis is difficult due to the variety of symptoms of the disease. In addition, animals may remain asymptomatic for life. In this study, the frequency of anti Leishmania chagasi antibodies was observed in 201 dogs referred to the clinical routine of the Veterinary Hospital of the Federal University of Mato Grosso, Campus of Sinop, during a 67 days period, through immunochromatographic method (DPP). Obtained results showed that seven animals were positive while one hundred ninety four were negative to the test. This result is relevant as far as it may increase the risk of CVL dispersion to non-endemic areas, highlighting the need of implementation effective measures to quickly detect Leishmania chagasi and prevent epidemiological situations.

scholarly journals Molecular detection of Anaplasma species in dogs in Colombia

2016 ◽  
Vol 25 (4) ◽  
pp. 459-464 ◽  
Author(s):  
Giovanni Vargas-Hernandez ◽  
Marcos Rogério André ◽  
Diana Maria Cendales ◽  
Keyla Carstens Marques de Sousa ◽  
Luiz Ricardo Gonçalves ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Low Frequency ◽  
Molecular Techniques ◽  
Western Region ◽  
Anaplasma Platys ◽  
Blood Samples ◽  
Granulocytic Anaplasmosis ◽  
Detection And Identification ◽  
Blood Smears ◽  
Canine Granulocytic Anaplasmosis

Abstract Anaplasma platys and A. phagocytophilum are tick-borne pathogens that parasitize platelets and neutrophils, respectively, of humans and animals. The former is the etiological agent of canine cyclic thrombocytopenia, while the latter is that of canine granulocytic anaplasmosis. This work involved the detection and identification of Anaplasma species in blood samples from dogs in Colombia, using molecular techniques. Between December 2008 and April 2009, blood samples were drawn from the cephalic vein of 91 dogs in the central-western region of Colombia (cities of Bogota, Villavicencio and Bucaramanga) and stored in tubes containing EDTA. These samples were used in 16S rRNA-Anaplasma spp. nPCR and the preparation of blood smears. One (1.1%) of the 91 sampled dogs showed inclusions suggestive of Anaplasmataceae agents in the cytoplasm of platelets. Based on PCR followed by sequencing and phylogenetic analysis, A. platys and Anaplasma sp. closed related to A. phagocytophilum were detected in two and one dog, respectively. Interestingly, all the samples were negative for specific msp-2-A. phagocytophilum real-time qPCR, suggesting the circulation of an Anaplasma species phylogenetically related to A. phagocytophilum in dogs in the aforementioned region. Hence, Anaplasma spp. circulates among dogs in Colombia, albeit with low frequency. To the best of authors' knowledge, this is the first molecular detection of Anaplasma spp. in dogs in Colombia.

scholarly journals Death in students’ everyday lives in 1930’s Brazil

2019 ◽  
Vol 6 (1) ◽  
pp. 245-263
Author(s):  
Kênia Hilda Moreira ◽  
Elizabeth Figueiredo De Sá
Keyword(s):  
20Th Century ◽  
Medical Science ◽  
History Of Education ◽  
Western Region ◽  
School Life ◽  
Student Body ◽  
Mato Grosso ◽  
History Of ◽  
The City ◽  
Education In Brazil

This article analyses the representations of death in different articles published in Vida Escolar («School Life») – a pedagogical journal produced by a student body of the city of Campo Grande, Mato Grosso state, central Western Brazil. Published by the Visconde de Taunay School, Vida Escolar circulated in the 1930s (between 1934 and 1936), and we analysed nineteen of the 22 published issues – archived at the Regional Documentation Center of the Federal University of Grande Dourados – for this article. This research helps to fill the gaps in knowledge surrounding the themes of death and childhood in the history of education in Brazil, especially in the central Western region. It reveals that the representations of death presented by and for children are closely related to civism, religiosity and medical science. Indeed, analysis of the journal Vida Escolar shows that death, as an interdiction, is related to the advancement of medicine; at the beginning of the 20th century, with the development of medical science, death came to be seen as synonymous with failure, as an absence of «scientificity». As emphasized in Vida Escolar, it is the job of medicine to prevent death – this was the message given to the students, future doctors.

scholarly journals Molecular detection of Ehrlichia canis and Anaplasma platys in dogs from municipality of Belém, State of Pará, Brazil

2019 ◽  
Vol 49 (12) ◽  
Author(s):  
Verucia Maria Dias Brandão ◽  
Pedro Henrique Marques Barrozo ◽  
Luciane Oeiras Sousa ◽  
Rafaelle Cunha dos Santos ◽  
Katiane Schwanke ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Molecular Detection ◽  
Ehrlichia Canis ◽  
Anaplasma Platys ◽  
Blood Samples ◽  
Stray Dogs ◽  
Significant Difference ◽  
The City

ABSTRACT: The occurrence of diseases transmitted by ticks in dogs is very frequent in Brazil, among these diseases we can highlight the ehrlichiosis and anaplasmosis, which are caused by Ehrlichia canis and Anaplasma platys, respectively. The objective of this study was to survey the occurrence of these pathogens in blood samples from domiciled and stray dogs from the city of Belém, Pará. Two hundred and seventy six dogs were sampled for convenience, and the DNA extracted from the blood of these animals was submitted to nested-PCR for research of E. canis and A. platys. E. canis DNA was detected in 39.4% (109/276) and A. platys DNA in 23.1% (64/276) of the samples, there was a statistically significant difference between the frequency of these agents (P<0.0001), and there was coinfection in 13.4% (37/276) of animals. The frequency of detection of these parasites was higher in stray dogs than in those domiciled for both E. canis (OR=2.84) and A. platys (OR=10.5). Considering the results, it was possible to conclude that E. canis and A. platys are present in the studied population, with stray dogs being more affected by these parasites.

scholarly journals Molecular detection of Mayaro virus during a dengue outbreak in the state of Mato Grosso, Central-West Brazil

2014 ◽  
Vol 109 (6) ◽  
pp. 820-823 ◽  
Author(s):  
Nayara Zuchi ◽  
Letícia Borges da Silva Heinen ◽  
Marcelo Adriano Mendes dos Santos ◽  
Fernanda Carla Pereira ◽  
Renata Dezengrini Slhessarenko
Keyword(s):  
Molecular Detection ◽  
The State ◽  
Mato Grosso ◽  
Dengue Outbreak ◽  
Mayaro Virus

scholarly journals Molecular diagnosis of Hepatozoon canis in symptomatic dogs in the city of Goiania, Goiás, Brazil

2016 ◽  
Vol 68 (6) ◽  
pp. 1431-1439
Author(s):  
S.C. Duarte ◽  
J.A. Parente ◽  
O.J. Silveira Neto ◽  
V.S. Jayme ◽  
T.S.A. Bastos ◽  
...  
Keyword(s):  
Laboratory Diagnosis ◽  
Extraction Process ◽  
Hepatozoon Canis ◽  
Phylogenetic Study ◽  
Blood Smears ◽  
Two Samples ◽  
Pcr Products ◽  
Hepatozoon Americanum ◽  
The City ◽  
Veterinary Hospital

ABSTRACT More than 300 species have been described in the genus Hepatozoon, occurring in different vertebrates. Among these, only Hepatozoon canis and Hepatozoon americanum are seen in dogs. Different methods may be used for laboratory diagnosis. The most common of these is direct parasitological examination of parasite stages in blood smears. The aim of this investigation was to conduct a phylogenetic study on Hepatozoon isolates from symptomatic dogs in the city of Goiânia, Goiás, Brazil. Blood samples were obtained from 40 symptomatic dogs that had been referred to the Veterinary Hospital of the Federal University of Goiás. Among these, only two samples were positive for Hepatozoon spp. using the direct parasitological method. These samples were then subjected to a DNA extraction process and amplification of a fragment of the 18S rRNA by means of PCR. Subsequently, the PCR products from each sample were purified and sequenced. The sequences obtained were then analyzed using the BLASTn algorithm, which identified both sequences of this study as Hepatozoon canis. By applying the Mega4 software, it was confirmed that these isolates of H. canis from dogs in Goiânia are similar to other reference isolates of the same species from other regions of Brazil and worldwide.

scholarly journals Endoparasites of wild animals from three biomes in the State of Mato Grosso, Brazil

2016 ◽  
Vol 68 (3) ◽  
pp. 571-578 ◽  
Author(s):  
D.G.S. Ramos ◽  
A.R.G.L.O. Santos ◽  
L.C. Freitas ◽  
S.H.R. Correa ◽  
G.V. Kempe ◽  
...  
Keyword(s):  
Host Species ◽  
Abiotic Factors ◽  
Wild Animals ◽  
New Host ◽  
Biotic Factor ◽  
Mato Grosso ◽  
Biotic And Abiotic Factors ◽  
Parasitological Examination ◽  
Biotic Potential ◽  
Veterinary Hospital

ABSTRACT The population of wild animals is regulated by many biotic and abiotic factors, and parasites are a biotic factor that affects the dynamic and density of host populations. From 2002 to 2014, 62 wild animals from the biomes Pantanal, Amazon, and "Cerrado" (or Savanna), which died in attendance in the veterinary hospital or have been road-killed, underwent necropsy for parasitological examination. Overall, 36 species of parasites were identified from 24 host species. Among the parasites, the most prevalent order was Oxyurida (29.1%), followed by Strongylida (20.9%), Spirurida (19.4%), Ascaridida (16.2%), Pentastomida (3.2%), Echinostomida (3.2%), Gygantorhynchia (3.2%), Rhabditida (1.6%), Plagiorchiida (1.6%), and Monilimorfida (1.6%), especially nematodes, which have more biotic potential and is more easily adapted to the environment than other classes. The occurrence of endoparasites was observed more frequently in endothermic than ectothermic animals, and herein is reported eleven new host occurrences for endoparasites in wild animals. The study has contributed to the knowledge on the biodiversity of parasites in wild animals from three biomes in central-western Brazil.

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