scholarly journals Effective Antioxidant activity of Phytic acid Prevents Isoproterenol-Induced Myocardial Infarction in Albino wistar Rats

2021 ◽  
Author(s):  
E BRINDHA
Keyword(s):  
Phytic Acid ◽  
Serum Iron ◽  
Wistar Rats ◽  
Binding Capacity ◽  
Iron Binding ◽  
Marker Enzymes ◽  
Total Proteins ◽  
Cardiac Marker ◽  
Iron Binding Capacity ◽  
G Ratio

Abstract This study aimed to evaluate the preventive effect of phytic acid on cardiac markers, blood glucose, total proteins, albumin/globulin (A/G) ratio, serum uric acid, serum iron, and plasma iron binding capacity in isoproterenol (ISO)-induced myocardial infarction (MI) in male Wistar rats. Rats subcutaneously injected with ISO (85 mg/kg) at an interval of 24 h for 2 days showed a significant increase in the degrees of cardiac troponin T (cTnT), intensity of the bands of lactate dehydrogenase (LDH)-isoenzyme (LDH1 and LDH2) and the activities of cardiac marker enzymes such as creatine kinase-MB (CK-MB), creatine kinase (CK), lactate dehydrogenase (LDH), aspartate transaminase (AST) and alanine transaminase (ALT) in serum with subsequent decrease in the activities of CK, LDH, AST and ALT in the heart. ISO-induced rats showed a significant increase in blood glucose, serum uric acid, serum iron and a decrease in the levels of total proteins, A/G ratio and iron binding capacity. Pretreatment with phytic acid (25 and 50 mg/kg) daily for a period of 56 days positively altered the levels of cTnT, intensity of the bands of the LDH1 and LDH2-isoenzyme and the activities of cardiac marker enzymes, and other biochemical parameters. Thus, phytic acid possess cardioprotective effect in ISO-induced oxidative stress in rats.

scholarly journals Interference of deferasirox with assays for serum iron and serum unsaturated iron binding capacity during iron chelating therapy

2011 ◽  
Vol 412 (23-24) ◽  
pp. 2261-2266 ◽  
Author(s):  
Katsuya Ikuta ◽  
Satoshi Ito ◽  
Hiroki Tanaka ◽  
Katsunori Sasaki ◽  
Yoshihiro Torimoto ◽  
...  
Keyword(s):  
Serum Iron ◽  
Binding Capacity ◽  
Iron Binding ◽  
Iron Binding Capacity ◽  
Chelating Therapy

Serum Iron and Serum Iron-Binding Capacity

1963 ◽  
pp. 139-150 ◽  
Author(s):  
THOMAS J. GIOVANNIELLO ◽  
THEODORE PETERS ◽  
EDWARD WAGMAN
Keyword(s):  
Serum Iron ◽  
Binding Capacity ◽  
Iron Binding ◽  
Iron Binding Capacity

Serum iron and total iron-binding capacity compared with serum ferritin in assessment of iron deficiency.

1981 ◽  
Vol 27 (2) ◽  
pp. 276-279 ◽  
Author(s):  
F Peter ◽  
S Wang
Keyword(s):  
Iron Deficiency ◽  
Serum Ferritin ◽  
Serum Iron ◽  
Binding Capacity ◽  
Transferrin Saturation ◽  
Total Iron ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Iron Deficient ◽  
Iron Binding Capacity

Abstract Ferritin values for 250 selected sera were compared with values for iron, total iron-binding capacity (TIBC), and transferrin saturation, to assess the potential of the ferritin assay for the detection of latent iron deficiency. The specimens were grouped (50 in each group) according to their values for iron and TIBC. In Group 1 (low iron, high TIBC) the saturation and ferritin values both indicated iron deficiency in all but one. In the 100 specimens of Groups 2 (normal iron, high TIBC) and 4 (normal iron, high normal TIBC), the saturation values revealed 16 iron-deficient cases, the ferritin test 55. For Groups 3 (low iron, normal TIBC) and 5 (low iron, low TIBC), the ferritin test revealed fewer cases of iron deficiency than did the saturation values (37 cases vs 51 cases, in the 100 specimens). Evidently the ferritin test detects iron deficiency in many cases for whom the serum iron and TIBC tests are not positively indicative. The correlation of serum ferritin with iron, TIBC, and transferrin saturation in the five groups was good only in the case of specimens for which the TIBC was normal; if it was abnormal the correlation was very poor.

Use of an Anion-Exchange Column to Determine Serum Iron-Binding Capacity

1971 ◽  
Vol 17 (9) ◽  
pp. 941-947 ◽  
Author(s):  
H Peter Lehmann ◽  
Alex Kaplan
Keyword(s):  
Serum Iron ◽  
Binding Capacity ◽  
Ferric Citrate ◽  
Iron Binding ◽  
Resin Column ◽  
Anion Exchange Column ◽  
Excess Iron ◽  
Colorimetric Measurement ◽  
Color Reagent ◽  
Iron Binding Capacity

Abstract A rapid procedure is described for determining total iron-binding capacity (TIBC) by use of a small, disposable resin column. Serum is saturated with excess ferric citrate, and iron not bound to protein is then removed by passing the mixture through the column of anion-exchanger. Transferrin-bound iron in the effluent, the TIBC, is determined colorimetrically (AutoAnalyzer), with 2,4,6-tripyridyl-1,3,5-triazine as color reagent. The percentage saturation with iron may be obtained from colorimetric measurement of original serum iron and the TIBC, or by radioactive counting if 59Fe is incorporated in the ferric citrate used to saturate the serum. Our method is compared with the MgCO3 precipitation technique for the removal of excess iron after saturation and to an ultrafiltration technique in which protein-bound iron is retained by a membrane and the filtrate analyzed for unbound iron, thus obviating absorbents in the determination of TIBC. Results from the resin-column and ultrafiltration procedures correlated well, whereas those from the MgCO3 method were consistently lower and more erratic.

scholarly journals NEOPTERIN AND INTERFERON-GAMMA AS IMMUNE RESPONSE MARKERS IN BETATHALASSEMIA MAJOR PATIENTS

2018 ◽  
Vol 11 (9) ◽  
pp. 192
Author(s):  
Shahad F Obeid ◽  
Sanad B Al-a'araji ◽  
Bassam F Matti ◽  
Hayder Adnan Fawzi
Keyword(s):  
Immune System ◽  
Interferon Gamma ◽  
Serum Iron ◽  
Binding Capacity ◽  
Iron Status ◽  
System Response ◽  
Iron Binding ◽  
Ifn Γ ◽  
Immune System Response ◽  
Iron Binding Capacity

Objective: The assessment of neopterin and interferon-gamma (IFN-γ) levels as a part of immune system response about serum iron status in β-thalassemia (TM) major patients.Methods: Spectrophotometry applied for the estimation of iron status including serum iron level, total iron-binding capacity, and unsaturated iron-binding capacity. Enzyme-linked immunosorbent assay (ELISA) applied for the estimation of the serum cytokines included neopterin and IFN-γ also serum ferritin estimation by ELISA from 130 β-TM major patients where they divided according to serum ferritin level (< and ≥2500 ng/mL).Results: The neopterin and IFN-γ concentration showed significantly increased with direct correlation among TM patient group when compared to the normal healthy control group. However, there was no significant difference between different levels of serum ferritin.Conclusion: The increased serum level of neopterin and IFN-γ found in patients with β-TM may be due to the direct effect of iron on cellular immune cells beside of immune system response to the effect of iron toxicity on different body organs. There was a direct moderate relationship between neopterin and IFN-γ.

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