scholarly journals Epistatic Interaction Effect Between Chromosome 1BL (Yr29) and A Novel Locus on 2AL Facilitating Resistance to Stripe Rust in Chinese Wheat Changwu 357-9

2021 ◽  
Author(s):  
Shuo Huang ◽  
Yibo Zhang ◽  
Hui Ren ◽  
Xiang Li ◽  
Xin Zhang ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Epistatic Interaction ◽  
Rust Resistance ◽  
Yellow Rust ◽  
Snp Array ◽  
Snp Markers ◽  
Stripe Rust Resistance ◽  
Disease Resistance Gene ◽  
Wheat Cultivars

Abstract Stripe rust (yellow rust) is a serious disease of bread wheat ( Triticum aestivum L.) worldwide. Genetic resistance is considered the most economical, effective and environmentally friendly method to control the disease. The current study focused on characterizing the components of stripe rust resistance and understanding the interactions in Changwu 357-9 (CW357-9)/Avocet S RIL population. A genetic linkage map constructed using a new GenoBaits Wheat 16K Panel and the 660K SNP array had 5,104 polymorphic SNP markers spanning 3,533.11 cM. Four stable QTL were detected on chromosome arms 1BL, 2AL, 3DS, and 6BS across all environments in Chngwu357-9. The most effective QTL Q YrCW357-1BL was Yr29 and the 6BS QTL was identified as Yr78 . The novel QTL on 2AL with moderate effect showed significant epistatic interaction with Yr29 . The QTL on 3DL should be same as QYrsn.nwafu-3DL and enriches the overall stripe rust resistance gene pool for breeding. Polymorphisms of flanking AQP markers AX-110020417 (for QYrCW357-1BL ), AX-110020417 (for QYrCW357 -2AL ), AX-109466386 (for QYrCW357 -3DL ), and AX-109995005 (for QYrCW357 -6BS ), were evaluated in a diversity panel including 225 wheat cultivars and breeding lines. The results suggested that these high-throughput markers could be used to introduce QYrCW357-1BL ( Yr29 ), QYrCW357-2AL , QYrCW357 -3DL , and QYrCW357 -6BS into commercial wheat cultivars. Combinations of these genes with other APR QTL should lead to higher levels of stripe rust resistance along with the beneficial effects of multi-disease resistance gene Yr29 on improving resistance to other diseases.

scholarly journals Genome-wide Mapping for Stripe Rust Resistance Loci in Common Wheat Cultivar Qinnong 142

Plant Disease ◽  
2019 ◽  
Vol 103 (3) ◽  
pp. 439-447 ◽  
Author(s):  
Qingdong Zeng ◽  
Jianhui Wu ◽  
Shengjie Liu ◽  
Xianming Chen ◽  
Fengping Yuan ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Wheat Cultivar ◽  
Infection Type ◽  
Snp Markers ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Wheat Cultivars

Stripe rust caused by Puccinia striiformis f. sp. tritici threatens worldwide wheat production. Growing resistant cultivars is the best way to control this disease. Chinese wheat cultivar Qinnong 142 (QN142) has a high level of adult-plant resistance to stripe rust. To identify quantitative trait loci (QTLs) related to stripe rust resistance, we developed a recombinant inbred line (RIL) population from a cross between QN142 and susceptible cultivar Avocet S. The parents and 165 F6 RILs were evaluated in terms of their stripe rust infection type and disease severity in replicated field tests with six site-year environments. The parents and RILs were genotyped with single-nucleotide polymorphism (SNP) markers. Four stable QTLs were identified in QN142 and mapped to chromosome arms 1BL, 2AL, 2BL, and 6BS. The 1BL QTL was probably the known resistance gene Yr29, the 2BL QTL was in a resistance gene-rich region, and the 2AL and 6BS QTLs might be new. Kompetitive allele specific polymerase chain reaction markers developed from the SNP markers flanking these QTLs were highly polymorphic in a panel of 150 wheat cultivars and breeding lines. These markers could be used in marker-assisted selection for incorporating the stripe rust resistance QTL into new wheat cultivars.

scholarly journals Enhanced Stripe Rust Resistance Obtained by Combining A Widely Dispersed, Stable QTL with Yr30 on Chromosome Arms 4BL And 3BS

2021 ◽  
Author(s):  
Shengjie Liu ◽  
Xiaoting Wang ◽  
Yayun Zhang ◽  
Yangang Jin ◽  
Zhonghua Xia ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Recombinant Inbred Lines ◽  
Durable Resistance ◽  
Snp Array ◽  
Snp Markers ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Wheat Cultivars ◽  
Testing Environments

Abstract Cultivars with durable resistance are the most popular means to control wheat stripe rust. Durable resistance can be achieved by stacking multiple adult-plant resistance (APR) genes that individually have relatively smaller effect. Chinese wheat cultivars Ruihua 520 (RH520) and Fengdecun 12 (FDC12) confer partial APR to stripe rust across environments. One hundred and seventy recombinant inbred lines from the cross RH520 × FDC12 were used to determine the genetic basis of resistance and identify genomic regions associated with stripe rust resistance. Genotyping was carried out using 55K SNP array and eight quantitative trait loci (QTL) were detected on chromosomes 2AL, 2DS, 3BS, 4BL, 5BL (2) and 7BL (2) by inclusive composite interval mapping. Only QYr.nwafu-3BS from RH520 and QYr.nwafu-4BL.2 (named YrFDC12 for convenience) from FDC12 were stable across the four testing environments. QYr.nwafu-3BS is likely the pleiotropic resistance gene Sr2/Yr30. YrFDC12 was mapped in a 2.1 cM interval corresponding to 12 Mb and flanked by SNP markers AX-111121224 and AX-89518393. Lines harboring both Yr30 and YrFDC12 displayed higher resistance than the parents and expressed pseudo-black chaff (PBC) controlled by two loci Pbc1 and PbcFDC12, which were co-located with Yr30 and YrFDC12, respectively. Both marker-based and pedigree-based kinship analyses revealed that YrFDC12 was inherited from founder parent Zhou 8425B. Fifty-four other wheat cultivars shared the same haplotype of YrFDC12 region. These results suggest an effective pyramiding strategy to acquire highly effective, durable stripe rust resistance in breeding.

scholarly journals Physical Location of New Stripe Rust Resistance Gene(s) and PCR-Based Markers on Rye (Secale cereale L.) Chromosome 5 Using 5R Dissection Lines

Agronomy ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 498 ◽  
Author(s):  
Wei Xi ◽  
Zongxiang Tang ◽  
Jie Luo ◽  
Shulan Fu
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Secale Cereale ◽  
Rust Resistance ◽  
Wheat Breeding ◽  
Rust Resistance Gene ◽  
Stripe Rust Resistance ◽  
Wheat Cultivars ◽  
Stripe Rust Resistance Gene ◽  
Secale Cereale L

The rye (Secale cereale L.) 5R chromosome contains some elite genes that can be used to improve wheat cultivars. In this study, a set of 5RKu dissection lines was obtained, and 111 new PCR-based and 5RKu-specific markers were developed using the specific length amplified fragment sequencing (SLAF-seq) method. The 111 markers were combined with the 52 5RKu-specific markers previously reported, and 65 S. cereale Lo7 scaffolds were physically mapped to six regions of the 5RKu chromosome using the 5RKu dissection lines. Additionally, the 5RLKu arm carried stripe rust resistance gene(s) and it was located to the region L2, the same region where 22 5RKu-specific markers and 11 S. cereale Lo7 scaffolds were mapped. The stripe rust resistance gene(s) located in the 5RLKu arm might be new one(s) because its source and location are different from the previously reported ones, and it enriches the resistance source of stripe rust for wheat breeding programs. The markers and the S. cereale Lo7 scaffolds that were mapped to the six regions of the 5RKu chromosome can facilitate the utilization of elite genes on the 5R chromosome in the improvement of wheat cultivars.

Identification and Validation of SNP Markers Linked to the Stripe Rust Resistance Gene Yr5 in Wheat

Crop Science ◽  
2016 ◽  
Vol 56 (6) ◽  
pp. 3055-3065 ◽  
Author(s):  
Yukiko Naruoka ◽  
Kaori Ando ◽  
Peter Bulli ◽  
Kebede T. Muleta ◽  
Sheri Rynearson ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Snp Markers ◽  
Rust Resistance Gene ◽  
Stripe Rust Resistance ◽  
Stripe Rust Resistance Gene

scholarly journals Molecular Mapping of a Stripe Rust Resistance Gene in Spring Wheat Cultivar Zak

2009 ◽  
Vol 99 (10) ◽  
pp. 1209-1215 ◽  
Author(s):  
X. X. Sui ◽  
M. N. Wang ◽  
X. M. Chen
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Spring Wheat ◽  
Rust Resistance ◽  
Molecular Mapping ◽  
Puccinia Striiformis ◽  
Yellow Rust ◽  
Dominant Gene ◽  
The United States ◽  
Stripe Rust Resistance

Stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici, is one of the most devastating foliar diseases of wheat (Triticum aestivum) worldwide. Growing resistant cultivars is the best approach for control of the disease. Although the stripe rust resistance in spring wheat cv. Zak has been circumvented by a group of races of the pathogen predominant in the United States since 2000, the resistance genes in Zak were unknown. To identify and map the genes for resistance to stripe rust, Zak was crossed with susceptible wheat genotype ‘Avocet Susceptible’. Seedlings of the parents and F1, F2, and F3 progeny were tested with P. striiformis f. sp. tritici races PST-43 and PST-45 under controlled greenhouse conditions. Genetic analysis determined that Zak has a single dominant gene, designated as YrZak, conferring race-specific all-stage resistance. Resistance gene analog polymorphism (RGAP), simple sequence repeat (SSR), and sequence-tagged site (STS) techniques were used to identify molecular markers linked to YrZak. A linkage group of three RGAP, three SSR, and three STS markers was constructed for YrZak using 205 F3 lines. Amplification of the complete set of Chinese Spring nulli-tetrasomic lines with RGAP marker Xwgp102 indicated that YrZak is present on chromosome 2B. The three SSR markers further mapped YrZak to the long arm of chromosome 2B. Amplification of chromosome 2B deletion lines with SSR marker Xgwm501 further confirmed that YrZak is on chromosome 2BL. To determine the genetic distance between YrZak and Yr5, which also is present on chromosome 2BL, 300 F2 plants from cross Zak/Yr5 were tested with PST-43. Six susceptible plants were identified from the F2 population, indicating that YrZak and Yr5 are ≈42 centimorgans apart. The results of race reactions and chromosomal locations indicated that YrZak is different from previously identified genes for resistance to stripe rust. This gene should be useful in monitoring virulence changes in the pathogen population and in studying host–pathogen interactions.

Improving Stripe Rust Resistance and Agronomic Performance in Three Elite Wheat Cultivars by Using a Combination of Marker Detection and Phenotypic Selection

2021 ◽  
Author(s):  
qiang Yang ◽  
taohong Fang ◽  
xin Li ◽  
Suizhuang Yang ◽  
Zhensheng Kang ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Phenotypic Selection ◽  
Introgression Lines ◽  
Stripe Rust Resistance ◽  
Agronomic Performance ◽  
Wheat Cultivars ◽  
Improvement Programs ◽  
Marker Detection

Abstract Wheat is an important cereal crop globally and improvement programs reach a plateau from ever-evolving stripe rust pathogen. Stripe rust, caused by Puccinia striiformis f. sp. Tritici (PST), is a fungal disease that devastates global wheat production. This disease is most effectively restrained by developing and deploying cultivars with highly durable resistance. The utilization of resistant genes is essential for breeding resistant cultivars. To improve stripe rust resistance and achieve favorable agronomic performance in commercial cultivars, an adult plant resistance gene Yr48 was introgressed from PI610750 into three Chinese elite wheat cultivars Chuanmai 42, Lunxuan 987, and Bainongaikang 58 using a combination of marker detection and phenotypic selection.The three Chinese elite cultivars as the recipient parent were used to cross with the donor parent PI610750. Eighty-seven introgression lines (ILs) were gained from all three crosses in the F5 generation. To validate the introgression lines possessing Yr48, SSR markers previously reported to be linked to the resistance gene loci were used to detect its presence or absence in different genetic backgrounds. Resistance and effect of introgressed Yr48 were studied using F6 and F7 grain grown in the field. The ILs from three crosses were evaluated the agronomic traits and the stripe rust reaction following stringent phenotypic criteria. Both phenotype and genotype data confirmed the presence of Yr48. A combination of phenotypic selection and marker-assisted breeding revealed that 9 selected lines had more application prospects. Those ILs had increased TGW (4.5–31.1%) in infested environments compared to the recipient parents. New lines with improved disease resistance and better agronomic performance should have more advantages than the original cultivars in wheat breeding programs.

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