Isolation of Novel Vincristine and Vinblastine Producing Streptomyces Species from Catharanthus Roseus Rhizospheric Soil
Abstract Microorganisms could be used as efficient tools to protect high value therapeutic plants against overexploitation and climate change. This work aimed to isolate alkaloids producing endophytic and rhizospheric soil actinomycetes and fungi of Catharanthus roseus. From a total of eleven actinomycetes and eight fungi strains isolated by dilution and plate methods, six telluric actinomycetes were revealed to produce alkaloids according to the precipitation test of their extracts. Revelation by Thin Layer Chromatography method using vinblastine and vincristine standards on the basis of frontal reference values showed that the strain SC8 and the vinblastine, the strain SC7 and the vincristine displayed the same frontal references (0,89 and 0,88, respectively). Whole genome sequencing method showed that both strains belong to the genus Streptomyces with novel species.Moreover, metabolic pathways analysis from their genomes allowed to detect three enzymes involved in the biosynthesis of terpenoid backbone leading to that of terpenoid indole alkaloids.
