Toxicity Evaluation of Choline Ionic Liquid-Based Nanocarriers of Pharmaceutical Agent For Lung Treatment With Extra Antitumor Activity

Mapping Intimacies ◽

10.21203/rs.3.rs-1152816/v1 ◽

2021 ◽

Author(s):

Katarzyna Niesyto ◽

Magdalena Skonieczna ◽

Malgorzata Adamiec-Organisciok ◽

Dorota Neugebauer

Keyword(s):

Ionic Liquid ◽

Epithelial Cells ◽

Cell Line ◽

Small Cell Lung Carcinoma ◽

Annexin V ◽

Antibacterial Drug ◽

Cell Lung Carcinoma ◽

Lung Carcinoma Cell Line ◽

Apoptosis Assay

Abstract In vitro cytotoxicity evaluation of linear copolymer (LC) containing choline ionic liquid units and its conjugates with an antibacterial drug in anionic form, i.e. p-aminosalicylate (LC_PAS), clavulanate (LC_CLV), or piperacillin (LC_PIP) was carried out. These systems were tested against normal: human bronchial epithelial cells (BEAS-2B), and cancers: adenocarcinoma human alveolar basal epithelial cells (A549), and human non-small cell lung carcinoma cell line (H1299). Cells viability, after linear copolymer LC and their conjugates addition for 72 hours, was measured at concentrations range of 3.125‑100 μg/mL. The MTT test allowed the designation of IC50 index, which was higher for BEAS-2B, and significantly lower in the case of cancer cell lines. The cytometric analyzes, i.e. Annexin-V FITC apoptosis assay and cell cycle analysis as well as gene expression measurements for interleukins IL6 and IL8 were carried out, and showed pro-inflammatory activity of tested compounds towards cancer cells, while it was not observed against normal cell line. In summary, the anticancer property of linear copolymers with antibacterial drugs was shown.

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Adsorption of cisplatin on oxidized graphene nanoribbons for improving the uptake in non-small cell lung carcinoma cell line A549

Current Drug Delivery ◽

10.2174/1567201818666210708124424 ◽

2021 ◽

Vol 18 ◽

Author(s):

Steffi Augustine ◽

Bala Prabhakar ◽

Pravin Shende

Keyword(s):

Cell Line ◽

Lung Carcinoma ◽

Carcinoma Cell ◽

Graphene Nanoribbons ◽

Small Cell Lung Carcinoma ◽

Small Cell ◽

Small Cell Lung ◽

Cell Lung Carcinoma ◽

Lung Carcinoma Cell Line

Background : Graphene nanoribbons are nanosized strips of graphene with unique physicochemical properties like higher drug loading capacity and affinity for tumor cells. Objective: The principal objective of this research was to develop oxidized graphene nanoribbons (O-GNRs)-a based delivery system for cisplatin against non-small cell lung carcinoma cell line A549 by selective endocytosis. Method: The O-GNRs prepared using various synthetic steps like oxidative unzipping were evaluated for various parameters like morphology, Fourier Transform Infrared (FTIR) study, % adsorption efficacy, Differential scanning colometric (DSC) study, and in-vitro efficacy studies. Results: Graphene nanoribbons with the length of 200-250 nm and width of 20-40 nm were obtained. The FTIR spectrum of drug-loaded O-GNRs exhibited a characteristic peak at 1550 cm-1 (-N-H group) of cisplatin. The DSC indicated the presence of sharp endothermic peaks at 59 ºC (PEG), 254 ºC (-C-NH3) and 308.6 ºC (-C-Pt). The % adsorption efficiency was found to be 74.56 ± 0.798% with in-vitro release in controlled manner (63.36 % ± 0.489 %) for 24 h. Conclusion: The nanoformulation showed an average inhibition of 22.72% at a lower dose of cisplatin (> 25%) by passive targeting cell line A549 by DNA alkylation. In the near future, graphene-based systems will establish potential nanosystems in cancer treatment due to the additive effect of graphene with various therapeutic agents.

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