A Bayesian Network Structure Learning Approach to Identify Genes Associated with Stress in Spleens of Chickens
Abstract Differences in the expression patterns of genes have been used to measure the effects of non-stress or stress conditions in poultry species. However, the list of genes identified can be extensive and they might be related to several biological systems. Therefore, the aim of this study was to identify a small set of genes closely associated with stress in a poultry animal model, the chicken (Gallus gallus), by reusing and combining data previously published together with bioinformatic analysis and Bayesian networks in a multi-step approach. Two datasets were collected from publicly available repositories and pre-processed. Bioinformatics analyses were performed to identify genes common to both datasets that showed differential expression patterns between non-stress and stress conditions. Bayesian networks were learnt using a Simulated Annealing algorithm implemented in the software Banjo. The structure of the Bayesian network consisted of 16 out of 19 genes in addition to the stress condition. CARD19 displayed a direct relationship with the stress condition, and three other genes, CYGB, BRAT1, and EPN3 were also relevant for the stress condition. The biological functionality of these genes are related to damage, apoptosis, and oxygen provision, and they could potentially be further explored as biomarkers of stress.