Hepatic Progenitor Cells Promote the Repair of Schistosomiasis Liver Injury Through Inhibiting IL-33 Secretion

Abstract Background: Hepatic schistosomiasis, a chronic liver injury induced by long-term Schistosoma japonicum (S. japonicum) infection, is characterized by egg granulomas and fibrotic pathology. Hepatic progenitor cells (HPCs), which are nearly absent and quiescent in normal liver, play vital roles in chronic and severe liver injury. But their role in the progression of liver injury during infection remained unknown.Methods: In this study, the hepatic egg granulomas, fibrosis and proliferation of HPCs were analyzed in S. japonicum infection mice model at different infection stages. For validating the role of HPCs in hepatic injury, TNFrelated weak inducer of apoptosis (TWEAK) and TWEAK blocking antibody were used to manipulate the proliferation of HPCs. Histologic pathology and the expression of IL-33 were examined. Results: We found that the proliferation of HPCs paralleled with inflammatory granulomas and fibrosis formation. Promoting HPCs expansion promote the liver regeneration and inhibit the hepatocytes injury, the inflammatory eggs granulomas and the deposition of fibrotic collagen. Interestingly, the expression of IL-33 decreased when HPCs were manipulated to proliferate. Thus, IL-33 might be involved in the liver repair dominated by HPCs. Conclusions: Collectively, our data uncovered a protective role of HPCs in hepatic schistosomiasis in an IL-33 related manner, which might provide a promising progenitor cell therapy for hepatic schistosomiasis.

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Hepatic progenitor cells promote the repair of schistosomiasis liver injury by inhibiting IL-33 secretion in mice

Stem Cell Research & Therapy ◽

10.1186/s13287-021-02589-y ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Beibei Zhang ◽

Xiaoying Wu ◽

Jing Li ◽

An Ning ◽

Bo Zhang ◽

...

Keyword(s):

Liver Injury ◽

Progenitor Cells ◽

Normal Liver ◽

Protective Role ◽

Dependent Manner ◽

Hepatic Progenitor Cells ◽

Wild Type ◽

Mice Model

Abstract Background Hepatic schistosomiasis, a chronic liver injury induced by long-term Schistosoma japonicum (S. japonicum) infection, is characterized by egg granulomas and fibrotic pathology. Hepatic progenitor cells (HPCs), which are nearly absent or quiescent in normal liver, play vital roles in chronic and severe liver injury. But their role in the progression of liver injury during infection remains unknown. Methods In this study, the hepatic egg granulomas, fibrosis and proliferation of HPCs were analyzed in the mice model of S. japonicum infection at different infectious stages. For validating the role of HPCs in hepatic injury, tumor necrosis factor-like-weak inducer of apoptosis (TWEAK) and TWEAK blocking antibody were used to manipulate the proliferation of HPCs in wild-type and IL-33−/− mice infected with S. japonicum. Results We found that the proliferation of HPCs was accompanied by inflammatory granulomas and fibrosis formation. HPCs expansion promoted liver regeneration and inhibited inflammatory egg granulomas, as well as the deposition of fibrotic collagen. Interestingly, the expression of IL-33 was negatively associated with HPCs’ expansion. There were no obvious differences of liver injury caused by infection between wild-type and IL-33−/− mice with HPCs’ expansion. However, liver injury was more attenuated in IL-33−/− mice than wild-type mice when the proliferation of HPCs was inhibited by anti-TWEAK. Conclusions Our data uncovered a protective role of HPCs in hepatic schistosomiasis in an IL-33-dependent manner, which might provide a promising progenitor cell therapy for hepatic schistosomiasis.

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The Progenitor Cell Compartment in the Feline Liver: An (Immuno)Histochemical Investigation

Veterinary Pathology ◽

10.1354/vp.07-vp-0097-i-fl ◽

2009 ◽

Vol 46 (4) ◽

pp. 614-621 ◽

Cited By ~ 15

Author(s):

J. Ijzer ◽

J. R. Kisjes ◽

L. C. Penning ◽

J. Rothuizen ◽

T. S. G. A. M Van Den Inch

Keyword(s):

Progenitor Cells ◽

Progenitor Cell ◽

Normal Liver ◽

Hepatic Progenitor Cells ◽

Ductular Reaction ◽

Hepatic Diseases ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Mitotic Figures ◽

Progenitor Compartment

The hepatic progenitor compartment is of vital importance in liver regeneration when hepatocellular replication is impaired, as it occurs in acute fulminant hepatitis or severe liver fibrosis. It consists of resident progenitor cells in the normal liver, and ductular reaction and intermediate hepatobiliary cells in diseased livers. An histologic and immunohistochemical study was conducted to demonstrate putative hepatic progenitor cells in the normal liver (n = 5) and in a range of hepatic diseases (n = 13) in the cat. Formalin-fixed, paraffin-embedded specimens were stained with HE, the van Gieson stain, and the reticulin stain according to Gordon and Sweet, and immunohistochemically stained for cytokeratin-7 (CK7), human hepatocyte marker 1 (Heparl), and multidrug resistance-binding protein-2/ATP binding cassette C2 (MRP2). The normal feline liver contains a liver progenitor cell morphologically similar to humans and dogs, which resides in the canal of Hering. In acute and chronic feline liver diseases a ductular reaction is present, whether in the parenchyma or in a portal or septal location. The putative progenitor cells could easily be demonstrated by staining for CK7, whereas they were generally negative for Heparl and MRP2. In a parenchymal ductular reaction mitotic figures and cells with an intermediate hepatobiliary phenotype could be demonstrated. This is the first account of hepatic progenitor cells in feline liver.

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Tetrachlorobenzoquinone induces acute liver injury, up-regulates HO-1 and NQO1 expression in mice model: The protective role of chlorogenic acid

Environmental Toxicology and Pharmacology ◽

10.1016/j.etap.2014.04.022 ◽

2014 ◽

Vol 37 (3) ◽

pp. 1212-1220 ◽

Cited By ~ 30

Author(s):

Demei Xu ◽

Lihua Hu ◽

Xiaomin Xia ◽

Jianbo Song ◽

Lingrui Li ◽

...

Keyword(s):

Liver Injury ◽

Chlorogenic Acid ◽

Acute Liver Injury ◽

Protective Role ◽

Mice Model

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A Protective Role of Okadaic Acid in Liver Injury Induced by Amoxicillin

Bulletin of Experimental Biology and Medicine ◽

10.1007/s10517-022-05386-6 ◽

2022 ◽

Author(s):

D. Li ◽

W. Shi ◽

X. Lu ◽

Z. Liu ◽

S. Zhang ◽

...

Keyword(s):

Liver Injury ◽

Okadaic Acid ◽

Protective Role

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Keratoprostheses in silicone oil-filled eyes: long-term outcomes

British Journal of Ophthalmology ◽

10.1136/bjophthalmol-2018-312426 ◽

2018 ◽

Vol 103 (6) ◽

pp. 781-788 ◽

Cited By ~ 2

Author(s):

Geetha Iyer ◽

Bhaskar Srinivasan ◽

Shweta Agarwal ◽

Ruchika Pattanaik ◽

Ekta Rishi ◽

...

Keyword(s):

Silicone Oil ◽

Vitreoretinal Surgery ◽

Retrospective Chart Review ◽

Protective Role ◽

Visual Rehabilitation ◽

Two Factors

PurposeTo analyse the functional and anatomical outcomes of different types of keratoprostheses in eyes with retained silicone oil following vitreoretinal surgery.MethodsRetrospective chart review of patients operated with any type of permanent keratoprosthesis (Kpro) in silicone oil-filled eyes between March 2003 and June 2017 were analysed.Results40 silicone oil-filled eyes underwent keratoprostheses, of which 22 were type 1 and 18 were type 2 Kpros (Lucia variant—nine, modified osteo odonto kerato prosthesis (MOOKP)—four, Boston type 2—three and osteoKpro—two) with a mean follow-up of 61.54 , 42.77, 45.25 , 25 and 37 months, respectively. Anatomic retention of the primary Kpro was noted in 33 eyes (82.5%). A best-corrected visual acuity of better than 20/200 and 20/400 was achieved in 26 (65%)+32 (80%) eyes. Retroprosthetic membrane (RPM) was the most common complication noted in 17 eyes (42.5%). Perioptic graft melt was noted in 4 of 22 eyes of the type 1 Kpro (2 (10.5%) without associated ocular surface disorder (OSD)) and in 1 eye each of Boston and Lucia type 2 Kpro. Laminar resorption occurred in one eye each of the MOOKP and OKP groups. Endophthalmitis and glaucoma did not occur in any eye.ConclusionAppropriately chosen keratoprosthesis is a viable option for visual rehabilitation in eyes post vitreoretinal surgery with retained silicone oil-induced keratopathy not amenable to conventional penetrating keratoplasty. Kpro melt among type 1 Kpro did not occur in 89.5% eyes without associated OSD (19 of 22 eyes), despite the lack of aqueous humour and presence of RPM (4 eyes), two factors considered to play a significant role in the causation of sterile melts. Of interest to note was the absence of infection in any of these eyes. The possible protective role of oil from endophthalmitis is interesting, though yet to be ascertained.

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Hepatic progenitor cells, stem cells, and AFP expression in models of liver injury

International Journal of Experimental Pathology ◽

10.1111/j.1365-2613.2006.00485.x ◽

2006 ◽

Vol 87 (5) ◽

pp. 343-359 ◽

Cited By ~ 56

Author(s):

Wolf D. Kuhlmann ◽

Peter Peschke

Keyword(s):

Stem Cells ◽

Liver Injury ◽

Progenitor Cells ◽

Hepatic Progenitor Cells

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Propylthiouracil-Induced Liver Injury in Mice and the Protective Role of Taurine

Pharmaceutical Sciences ◽

10.15171/ps.2015.23 ◽

2015 ◽

Vol 21 (2) ◽

pp. 94-101 ◽

Cited By ~ 4

Author(s):

Raziyeh Karamikhah ◽

Akram Jamshidzadeh ◽

Negar Azarpira ◽

Arastoo Saeidi ◽

Reza Heidari

Keyword(s):

Liver Injury ◽

Protective Role

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Protective role of Ficus benghalensis against isoniazid-rifampicin induced oxidative liver injury in rat

Revista Brasileira de Farmacognosia ◽

10.1590/s0102-695x2012005000009 ◽

2012 ◽

Vol 22 (3) ◽

pp. 604-610 ◽

Cited By ~ 1

Author(s):

S. Angala Parameswari ◽

T. S. Mohamed Saleem ◽

K. B Chandrasekar ◽

C. Madhusudhana Chetty

Keyword(s):

Liver Injury ◽

Protective Role ◽

Ficus Benghalensis

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Heterogeneity of murine periosteum osteochondroprogenitors involved in fracture healing

10.1101/2020.06.24.169003 ◽

2020 ◽

Author(s):

Brya G Matthews ◽

Francesca V Sbrana ◽

Sanja Novak ◽

Jessica L. Funnell ◽

Ye Cao ◽

...

Keyword(s):

Fracture Healing ◽

Progenitor Cells ◽

Progenitor Cell ◽

Callus Formation ◽

Lineage Tracing ◽

Fracture Callus ◽

Stem And Progenitor Cells ◽

Periosteal Cells ◽

Osteoblast Lineage

AbstractThe periosteum is the major source of cells involved in fracture healing. We sought to characterize differences in progenitor cell populations between periosteum and other bone compartments, and identify periosteal cells involved in fracture healing. The periosteum is highly enriched for progenitor cells, including Sca1+ cells, CFU-F and label-retaining cells. Lineage tracing with αSMACreER identifies periosteal cells that contribute to >80% of osteoblasts and ~40% of chondrocytes following fracture. A subset of αSMA+ cells are quiescent long-term injury-responsive progenitors. Ablation of αSMA+ cells impairs fracture callus formation. In addition, committed osteoblast-lineage cells contributed around 10% of osteoblasts, but no chondrocytes in fracture calluses. Most periosteal progenitors, particularly those that form osteoblasts, can be targeted by αSMACreER. We have demonstrated that the periosteum is highly enriched for skeletal stem and progenitor cells and there is heterogeneity in the populations of cells that contribute to mature lineages during periosteal fracture healing.

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