scholarly journals Identification of CRF89_BF, a new member of an HIV-1 circulating BF intersubtype recombinant form family widely spread in South America

2021 ◽  
Author(s):  
Elena Delgado ◽  
Aurora Fernández-García ◽  
Ismael Fernández-Miranda ◽  
Sonia Benito ◽  
Vanesa Montero ◽  
...  
Keyword(s):  
South America ◽  
Genome Sequence ◽  
Full Length ◽  
Substantial Proportion ◽  
Recombinant Form ◽  
South American ◽  
Sequence Analyses ◽  
Full Length Genome ◽  
South American Origin ◽  
Hiv 1

Abstract Circulating recombinant forms (CRFs) represent a substantial proportion of infections in the HIV-1 pandemic. Among 103 CRFs described in the literature, 16 are BF intersubtype recombinants, most of South American origin, of which CRF12_BF is the most widely spread. A BF recombinant cluster identified in Bolivia was suggested to represent a new CRF_BF. Here, we find that this cluster belongs to a larger cluster incorporating 38 viruses collected in 7 countries from 3 continents, 21 of them in Spain, most from Bolivian or Peruvian individuals, and 12 in South America (Bolivia, Argentina, and Peru). It comprises three major subclusters, two associated with Bolivian individuals and one associated with Peruvian individuals. Near full-length genome sequence analyses of five viruses collected in Spain, Bolivia, and Peru revealed coincident BF mosaic structures, with 13 breakpoints, 5 and 6 of which coincided with CRF12_BF and CRF17_BF, respectively, and grouping in a clade closely related to these CRFs and more distantly to CRF38_BF and CRF44_BF, all circulating in South America. These results allow us to identify a new HIV-1 CRF, designated CRF89_BF. Through phylodynamic analyses, CRF89_BF emergence was estimated in Bolivia around 1984. CRF89_BF is the fifth CRF member of the HIV-1 recombinant family related to CRF12_BF

scholarly journals Identification of CRF89_BF, a new member of an HIV-1 circulating BF intersubtype recombinant form family widely spread in South America

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Elena Delgado ◽  
Aurora Fernández-García ◽  
Marcos Pérez-Losada ◽  
María Moreno-Lorenzo ◽  
Ismael Fernández-Miranda ◽  
...  
Keyword(s):  
South America ◽  
Phylogenetic Tree ◽  
Genome Sequence ◽  
Full Length ◽  
Recombinant Form ◽  
South American ◽  
Sequence Analyses ◽  
Full Length Genome ◽  
South American Origin ◽  
Hiv 1

AbstractCirculating recombinant forms (CRFs) contribute substantially to the HIV-1 pandemic. Among 105 CRFs described in the literature, 16 are BF intersubtype recombinants, most of South American origin, of which CRF12_BF is the most widely spread. A BF recombinant cluster identified in Bolivia was suggested to represent a new CRF_BF. Here we find that it belongs to a larger cluster incorporating 39 viruses collected in 7 countries from 3 continents, 22 of them in Spain, most from Bolivian or Peruvian individuals, and 12 in South America (Bolivia, Argentina, and Peru). This BF cluster comprises three major subclusters, two associated with Bolivian and one with Peruvian individuals. Near full-length genome sequence analyses of nine viruses, collected in Spain, Bolivia, and Peru, revealed coincident BF mosaic structures, with 13 breakpoints, 6 and 7 of which coincided with CRF12_BF and CRF17_BF, respectively. In a phylogenetic tree, they grouped in a clade closely related to these CRFs, and more distantly to CRF38_BF and CRF44_BF, all circulating in South America. These results allowed to identify a new HIV-1 CRF, designated CRF89_BF. Through phylodynamic analyses, CRF89_BF emergence was estimated in Bolivia around 1986. CRF89_BF is the fifth CRF member of the HIV-1 recombinant family related to CRF12_BF.

scholarly journals Near Full-Length Genome Identification of a Novel HIV-1 Recombinant Form (CRF01_AE/B′/C) Among Heterosexuals in Jilin, China

2014 ◽  
Vol 30 (7) ◽  
pp. 695-700 ◽  
Author(s):  
Xingguang Li ◽  
Chuanyi Ning ◽  
Yanli Chen ◽  
Yi Feng ◽  
Min Wei ◽  
...  
Keyword(s):  
Full Length ◽  
Recombinant Form ◽  
Full Length Genome ◽  
Hiv 1

scholarly journals Identification of CRF66_BF, a new HIV-1 circulating recombinant form of South American origin

2021 ◽  
Author(s):  
Joan Bacque ◽  
Elena Delgado ◽  
Sonia Benito ◽  
Maria Moreno-Lorenzo ◽  
Vanessa Montero ◽  
...  
Keyword(s):  
Phylogenetic Trees ◽  
Recent Common Ancestor ◽  
South American ◽  
Most Recent Common Ancestor ◽  
Phylogenetic Cluster ◽  
Subtype B ◽  
Sex With Men ◽  
Full Length Genome ◽  
South American Origin ◽  
Hiv 1

Circulating recombinant forms (CRFs) are important components of the HIV-1 pandemic. Among 108 reported in the literature, 17 are BF1 intersubtype recombinant, most of which are of South American origin. Among these, all 5 identified in the Southern Cone and neighboring countries, except Brazil, derive from a common recombinant ancestor related to CRF12_BF, which circulates widely in Argentina, as deduced from coincident breakpoints and clustering in phylogenetic trees. In a HIV-1 molecular epidemiological study in Spain, we identified a phylogenetic cluster of 20 samples from 3 separate regions which were of F1 subsubtype, related to the Brazilian strain, in protease-reverse transcriptase (Pr-RT) and of subtype B in integrase. Remarkably, 14 individuals from this cluster (designated BF9) were Paraguayans and only 4 were native Spaniards. HIV-1 transmission was predominantly heterosexual, except for a subcluster of 6 individuals, 5 of which were men who have sex with men. Ten additional database sequences, from Argentina (n=4), Spain (n=3), Paraguay (n=1), Brazil (n=1), and Italy (n=1), branched within the BF9 cluster. To determine whether it represents a new CRF, near full-length genome (NFLG) sequences were obtained for 6 viruses from 3 Spanish regions. Bootscan analyses showed a coincident BF1 recombinant structure, with 5 breakpoints, located in p17gag, integrase, gp120, gp41-rev overlap, and nef, which was identical to that of two BF1 recombinant viruses from Paraguay previously sequenced in NFLGs. Interestingly, none of the breakpoints coincided with those of CRF12_BF. In a maximum likelihood phylogenetic tree, all 8 NFLG sequences grouped in a strongly supported clade segregating from previously identified CRFs and from the CRF12_BF family clade. These results allow us to identify a new HIV-1 CRF, designated CRF66_BF. Through a Bayesian coalescent analysis, the most recent common ancestor of CRF66_BF was estimated around 1984 in South America, either in Paraguay or Argentina. Among Pr-RT sequences obtained by us from HIV-1-infected Paraguayans living in Spain, 14 (20.9%) of 67 were of CRF66_BF, suggesting that CRF66_BF may be one of the major HIV-1 genetic forms circulating in Paraguay. CRF66_BF is the first reported non-Brazilian South American HIV-1 CRF_BF unrelated to CRF12_BF

Near Full-Length Genome Identification of a Novel HIV-1 Recombinant Form (CRF01_AE/CRF07_BC) in Zhejiang, China

2016 ◽  
Vol 32 (9) ◽  
pp. 900-903 ◽  
Author(s):  
Xiaorong Peng ◽  
Xu Li-jun ◽  
Tiansheng Xie ◽  
Fumin Liu ◽  
Nanping Wu
Keyword(s):  
Full Length ◽  
Recombinant Form ◽  
Full Length Genome ◽  
Hiv 1
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