scholarly journals An Ultrasensitive Immunoassay Strip for Simultaneously Detecting Cyproheptadinehydrochloride and Six Phenothiazinesin Feedstuffs Based on a Monoclonal Antibody

2020 ◽  
Author(s):  
Guanqiong Na ◽  
Xiaofei Hu ◽  
Yaning Sun ◽  
Liang Tang ◽  
Guangxu Xing ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Screening Method ◽  
Immunochromatographic Strip ◽  
Bromoacetic Acid ◽  
Animal Products ◽  
Food Animal ◽  
Inhibition Concentration ◽  
Artificial Antigen ◽  
Animal Feedstuffs ◽  
Harmful Effects

Abstract Background: An antihistamine cyproheptadine (CPH) and phenothiazines (PZs)sedative hypnoticshave a similar tricyclic structure, these drugs are often illegally added to food animal feedstuffs due to their price advantage and significant effect in promoting growth and improving meat quality. However, the abuse of these drugs may lead totheir residues in animal products, thereby causing harmful effects such as allergies and dermatological reactions on human health.To supervise the use of prohibited drugs and ensure food safety, it is necessary to establish a simple and effective screening method to detect CPH and PZs.In this research, an artificial antigen against cyproheptadine(CPH) was successfully synthesized by bromoacetic acid substitution method. An ultrasensitive and broad-specific monoclonal antibody (mAb) able to recognize CPH and six phenothiazines (PZs) was produced. Based on the gold-labeled mAb, an immunochromatographic strip (ICS) was established.Results:The 50% inhibition concentration (IC50) of the produced mAb against CPH was identified as 0.036 ng mL-1 by ELISA, and the cross-reactivities for six PZs were from 6.33% to 63.16%. The visual detection limits(cut-off values) of the developed ICS ranged from 5 to 100ngg-1 in feedstuffs. Take a reading by strip reader, the IC50 was from 0.570 to 7.750ng g-1. In addition,a recovery experiment was carried out to verify the reliability of the ICS. The intra-assay recoveries were from 79.83% to 103.38% with the highest coefficient of variation (CV) of 11.31%. The inter-assay recoveries were from 79.00% to 96.63% with the highest CV of 12.66%.Conclusions: We have successfully produced a broad-spectrum monoclonal antibody and established an ICS for simultaneouslydetecting CPH and six PZs drugs.In brief, the proposed ICS was considered suitable for qualitatively and quantitatively monitoring CPH and PZs in feedstuffs.

КЛИНИЧЕСКОЕ ЗНАЧЕНИЕ ПИЩЕВЫХ АЛЛЕРГЕНОВ ЖИВОТНОГО ПРОИСХОЖДЕНИЯ

2019 ◽  
Author(s):  
A.N. Pampura ◽  
E.E. Varlamov
Keyword(s):  
Clinical Manifestations ◽  
Red Meat ◽  
Cross Reactivity ◽  
Allergic Reactions ◽  
Essential Nutrients ◽  
Animal Products ◽  
Food Animal ◽  
Mammalian Meat

Продукты животного происхождения обладают наиболее сбалансированным комплексом необходимых нутриентов, поэтому их включение в пищевой рацион оправданно в различные возрастные периоды. Клинические проявления аллергии к данным продуктам крайне разнообразны. Одним из ключевых аспектов обозначенной проблемы является диагностика и оценка вероятности развития перекрестной реактивности. К пищевым продуктам животного происхождения, наиболее часто индуцирующим симптомы, относятся коровье молоко, куриное яйцо, рыба, моллюски, ракообразные, мясо млекопитающих. В представленной статье изложены данные об основных классах пищевых аллергенов животного происхождения, индуцирующих развитие перекрестных аллергических реакций. Описаны синдромы, связанные с перекрестной реактивностью: птица-яйцо , кошка-свинина , рыба-курица и -Gal-синдром/ синдром красного мяса .Animal foods products contain the most balanced complex of essential nutrients. The clinical manifestations of allergy to these products are varied. Key aspect of this problem is diagnostics and assessment of cross-reactivity. The food animal products that more often induce symptoms include cows milk, hens egg, fish, mollusks, crustaceans, mammalian meat. Data on the main classes of food animal allergens inducing the development of cross-allergic reactions as well as syndromes associated with cross-reactivity like bird-egg , cat-pork , fish-chicken and a-Gal-syndrome/ red meat syndrome are described in this article.

Development of a monoclonal antibody-based immunochromatographic strip for the rapid detection of tigecycline in human serum

2021 ◽  
Author(s):  
Chuanlai Xu ◽  
qianqian lu ◽  
Xin-Xin Xu ◽  
Shanshan Song ◽  
aihong wu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Serum ◽  
Rapid Detection ◽  
Immunochromatographic Assay ◽  
Immunochromatographic Strip

In this study, a gold labelled immunochromatographic assay was developed to detect tigecycline (TGC) in human serum. For this purpose, an anti-TGC monoclonal antibody, 2G7, was produced and characterized, and...

Simultaneous detection of phenacetin and its metabolite using a gold nanoparticle-based immunochromatographic test strip

The Analyst ◽  
2021 ◽  
Author(s):  
Jingjing Yao ◽  
Xin-Xin Xu ◽  
Liqiang Liu ◽  
Hua Kuang ◽  
Zhengyou Wang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Gold Nanoparticle ◽  
Simultaneous Detection ◽  
Test Strip ◽  
Immunochromatographic Test ◽  
Immunochromatographic Strip

We have developed a sensitive and rapid gold nanoparticle-based immunochromatographic strip (GNP-ICS) for the detection of phenacetin (PNCT) using an anti-PNCT monoclonal antibody (mAb). The specific anti- PNCT mAb (2D6)...

Occurrence of Plasmid-Mediated Quinolone Resistance Determinants andrmtBGene inSalmonella entericaSerovar Enteritidis and Typhimurium Isolated from Food-Animal Products in Tunisia

2013 ◽  
Vol 10 (9) ◽  
pp. 813-819 ◽  
Author(s):  
Nazek Al-Gallas ◽  
Mohamed Salah Abbassi ◽  
Becher Gharbi ◽  
Molka Manai ◽  
Mohamed N. Ben Fayala ◽  
...  
Keyword(s):  
Quinolone Resistance ◽  
Animal Products ◽  
Resistance Determinants ◽  
Food Animal

Monoclonal antibodies evaluated for use in a screening assay for conjugates of human serum albumin and pyridoxal 5'-phosphate.

1989 ◽  
Vol 35 (8) ◽  
pp. 1756-1760 ◽  
Author(s):  
B B Miller ◽  
W E Turner
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Human Serum Albumin ◽  
Human Serum ◽  
Limit Of Detection ◽  
Screening Method ◽  
Serum Samples ◽  
Screening Assay ◽  
Antibody Avidity ◽  
Negative Results

Abstract This enzyme immunoassay (EIA) was developed to measure pyridoxal 5'-phosphate bound to albumin (PLP-HSA) in human serum. The monoclonal antibody titer was 1:2000 and a sequential saturation analysis curve, prepared with samples containing from 10 to 1000 nmol/L, showed a 50% inhibition of antibody at 50 nmol of the conjugate per liter. The lower limit of detection for PLP-HSA was 10 nmol/L, a sensitivity 1000-fold greater than that for any potential interferent. When serum samples gave negative results in the assay, we compared the antigenicity of the principal sites for PLP binding on HSA. It was apparent that the preferred physiological site was not antigenic; however, three additional sites for PLP binding on HSA elicited comparable antibody avidity. This EIA is potentially quite sensitive and specific for PLP-HSA, but considerable additional effort is required to convert serum PLP to an HSA-bound form detectable in the assay, which limits its application as a screening method.

Development of a monoclonal antibody-based immunochromatographic strip for cephalexin

2014 ◽  
Vol 26 (2) ◽  
pp. 282-292 ◽  
Author(s):  
Jingnan Guo ◽  
Liqiang Liu ◽  
Feng Xue ◽  
Changrui Xing ◽  
Shanshan Song ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunochromatographic Strip
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