Simultaneous detection of phenacetin and its metabolite using a gold nanoparticle-based immunochromatographic test strip

The Analyst ◽  
2021 ◽  
Author(s):  
Jingjing Yao ◽  
Xin-Xin Xu ◽  
Liqiang Liu ◽  
Hua Kuang ◽  
Zhengyou Wang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Gold Nanoparticle ◽  
Simultaneous Detection ◽  
Test Strip ◽  
Immunochromatographic Test ◽  
Immunochromatographic Strip

We have developed a sensitive and rapid gold nanoparticle-based immunochromatographic strip (GNP-ICS) for the detection of phenacetin (PNCT) using an anti-PNCT monoclonal antibody (mAb). The specific anti- PNCT mAb (2D6)...

DEVELOPING AND ASSESSING AN IMMUNOCHROMATOGRAPHIC STRIP FOR DETECTING OSTEOARTHRITIS BASED ON URINE CARTILAGE OLIGOMERIC MATRIX PROTEINS

2014 ◽  
Vol 26 (06) ◽  
pp. 1450072 ◽  
Author(s):  
Yu-Hsien Kao ◽  
Kun-Lieh Wu ◽  
Yuan-Kun Tu ◽  
Shwu Jen Chang ◽  
Chin Chang Yang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Gold Nanoparticle ◽  
Matrix Protein ◽  
Cell Clone ◽  
Preliminary Test ◽  
Test Strip ◽  
Test Results ◽  
Immunochromatographic Strip ◽  
Western Blot Assay ◽  
Detection Range

An immunochromatographic strip was developed using a gold nanoparticle-conjugated monoclonal antibody to detect cartilage oligomeric matrix protein (COMP) in human urine. The monoclonal antibody anti-hCOMP produced from the hybridoma cell clone, hCOMP, is specific to osteoarthritis (OA), and polycolonal antibodies against COMP proteins were conjugated using a gold nanoparticle (approximately 40 nm) to enable detection. The preliminary test results of the proposed biomarker-prepared strip showed a positive correlation with those obtained using Western-blot assay to urinal COMP and exhibited a reliable detection range from 50 to 400 ng. The visual detection limit of the prepared test strip was 50 ng. The test results of OA patients showed consistent diagnostic agreement compared with clinical X-ray radiography. Thus, based on the detection of COMP from urine, the proposed immunoassay method is suitable for screening and noninvasively diagnosing OA.

Gold Nanoparticle‐Based Paper Sensor for Simultaneous Detection of 11 Benzimidazoles by One Monoclonal Antibody

Small ◽  
2017 ◽  
Vol 14 (6) ◽  
pp. 1701782 ◽  
Author(s):  
Lingling Guo ◽  
Xiaoling Wu ◽  
Liqiang Liu ◽  
Hua Kuang ◽  
Chuanlai Xu
Keyword(s):  
Monoclonal Antibody ◽  
Gold Nanoparticle ◽  
Simultaneous Detection ◽  
Paper Sensor

Rapid simultaneous detection of fumonisin B1 and deoxynivalenol in grain by immunochromatographic test strip

2020 ◽  
Vol 606 ◽  
pp. 113878
Author(s):  
Xi Huang ◽  
Xinyu Huang ◽  
Jianhua Xie ◽  
Xiujiang Li ◽  
Zhibing Huang
Keyword(s):  
Simultaneous Detection ◽  
Fumonisin B1 ◽  
Test Strip ◽  
Immunochromatographic Test

Development of a monoclonal antibody for the detection of xylazine in milk and its use in an immunochromatographic strip

2021 ◽  
Author(s):  
Mengjia Chao ◽  
Liqiang Liu ◽  
Aihong Wu ◽  
Shanshan Song ◽  
Xinxin Xu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Gold Nanoparticle ◽  
Limit Of Detection ◽  
Lateral Flow ◽  
Immunochromatographic Strip ◽  
Naked Eye ◽  
Milk Samples ◽  
Flow Test ◽  
Lateral Flow Test

A gold nanoparticle-based lateral-flow test (GNT) strip was developed to detect xylazine (XYL) in milk. And the limit of detection (LOD) and cut-off value of the GNT assay were evaluated to be 20 and 200 ng mL−1 in milk samples by the naked eye.

Production of a monoclonal antibody for the detection of vitamin B1 and its use in an indirect enzyme-linked immunosorbent assay and immunochromatographic strip

2020 ◽  
Vol 8 (9) ◽  
pp. 1935-1943 ◽  
Author(s):  
Lu Zeng ◽  
Xaioling Wu ◽  
Liqiang Liu ◽  
Liguang Xu ◽  
Hua Kuang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Colloidal Gold ◽  
Vitamin B1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vitamin B ◽  
Immunochromatographic Test ◽  
Immunochromatographic Strip

A monoclonal antibody (mAb) against vitamin B1 was prepared and based on this, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and colloidal gold-based immunochromatographic test (ICT) strip were developed.

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