High SMARCA5 Expression is Associated With Poor Prognosis in Patients With Non-Small Cell Lung Cancer
Abstract Background: SMARCA5 has been proved to play the role of oncogene in various types of tumors. The purpose of our study was to study the relationship between the expression of SMARCA5 and the prognosis of patients with Non-small cell lung cancer(NSCLC), and to investigate the effect of silencing the expression of SMARCA5 on the biological behavior of NSCLC. Methods:Immunohistochemistry method was adopted to detect to the expressions of SMARCA5 proteins in NSCLC and normal lung tissues. The correlation between the expression of SMARCA5 and clinicopathological factors and survival prognosis of patients with NSCLC were analyzed by statistical methods. Western blot and RT-PCR were used to detect the expression of SMARCA5 in normal human bronchial epithelial cell line (HBE) and human lung cancer cell lines (A549, Lu165, SK-MES-1 and NCI-H520). SMARCA5 siRNA was employed to silence the the expression of SMARCA5. MTT and Transwell methods were used to detect the proliferation and invasion of lung cancer cells.Results: SMARCA5 protein were highly expressed in 78 (59.5%) NSCLC tissues. SMARCA5 mRNA (P<0.05) and protein (P<0.05) were significantly higher in NSCLC tissues than in the adjacent normal tissues. SMARCA5 expression was significantly associated with poorly differentiation (P<0.001), advanced TNM stage (P<0.001), and pleural invasion (P=0.032). Meanwhile, High expression of SMARCA5 was associated with poor prognosis of NSCLC patients. Knockdown of SMARCA5 expression inhibited cell growth and invasion in NSCLC. Conclusion: High expression of SMARCA5 is associated with poor prognosis in patients with NSCLC, and SMARCA5 can increase activity of proliferation and invasion in NSCLC cells. SMARCA5 may play a decisive role in tumorigenesis of NSCLC.