Severe pantothenic acid deficiency induces alterations in the intestinal mucosal proteome of starter Pekin ducks

Abstract Background: Pantothenic acid deficiency (PAD) results in growth depression and intestinal hypofunction of animals. However, the underlying mechanisms remain to be established. Mucosal proteome might reflect dietary influences on physiological processes.Results: A total of 128 one-day-old Pekin ducks were divided into two groups, with 8 replicates and 8 birds per replicate. All the ducks were fed either a PAD or a pantothenic acid adequate (control, CON) diet. After a 16-day feeding period, two ducks from each replicate were sampled to measure plasma parameters, intestinal morphology, and mucosal proteome. High mortality, growth retardation, fasting hypoglycemia, reduced plasma insulin, and oxidative stress were observed in the PAD group compared to the CON group. Furthermore, PAD induced morphological alterations of the small intestine indicated by reduced villus height and villus surface area of duodenum, jejunum, and ileum. The duodenum mucosal proteome of ducks showed that 198 proteins were upregulated and 223 proteins were downregulated (> 1.5-fold change) in the PAD group compared to those in the CON group. Selected proteins were confirmed by Western blotting. Pathway analysis of these proteins exhibited the suppression of glycolysis and gluconeogenesis, fatty acid beta oxidation, tricarboxylic acid cycle, oxidative phosphorylation, oxidative stress, and intestinal absorption in the PAD group, indicating impaired energy generation and abnormal intestinal absorption. We also show that nine out of eleven proteins involved in regulation of actin cytoskeleton were upregulated by PAD, probably indicates reduced intestinal integrity.Conclusion: PAD leads to growth depression and intestinal hypofunction of ducks, which are associated with impaired energy generation, abnormal intestinal absorption, and regulation of actin cytoskeleton processes. These findings provide insights into the mechanisms of intestinal mucosa metabolic disorders due to PAD.

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Severe pantothenic acid deficiency induces alterations in the intestinal mucosal proteome of starter Pekin ducks

BMC Genomics ◽

10.1186/s12864-021-07820-x ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Jing Tang ◽

Yulong Feng ◽

Bo Zhang ◽

Yongbao Wu ◽

Zhanbao Guo ◽

...

Keyword(s):

Oxidative Stress ◽

Actin Cytoskeleton ◽

Intestinal Absorption ◽

Pantothenic Acid ◽

Energy Generation ◽

Growth Depression ◽

Pekin Ducks ◽

Acid Deficiency ◽

Regulation Of Actin Cytoskeleton ◽

Pantothenic Acid Deficiency

Abstract Background Pantothenic acid deficiency (PAD) results in growth depression and intestinal hypofunction of animals. However, the underlying molecular mechanisms remain to be elucidated. Mucosal proteome might reflect dietary influences on physiological processes. Results A total of 128 white Pekin ducks of one-day-old were randomly assigned to two groups, fed either a PAD or a pantothenic acid adequate (control, CON) diet. After a 16-day feeding period, two ducks from each replicate were sampled to measure plasma parameters, intestinal morphology, and mucosal proteome. Compared to the CON group, high mortality, growth retardation, fasting hypoglycemia, reduced plasma insulin, and oxidative stress were observed in the PAD group. Furthermore, PAD induced morphological alterations of the small intestine indicated by reduced villus height and villus surface area of duodenum, jejunum, and ileum. The duodenum mucosal proteome of ducks showed that 198 proteins were up-regulated and 223 proteins were down-regulated (> 1.5-fold change) in the PAD group compared to those in the CON group. Selected proteins were confirmed by Western blotting. Pathway analysis of these proteins exhibited the suppression of glycolysis and gluconeogenesis, fatty acid beta oxidation, tricarboxylic acid cycle, oxidative phosphorylation, oxidative stress, and intestinal absorption in the PAD group, indicating impaired energy generation and abnormal intestinal absorption. We also show that nine out of eleven proteins involved in regulation of actin cytoskeleton were up-regulated by PAD, probably indicates reduced intestinal integrity. Conclusion PAD leads to growth depression and intestinal hypofunction of ducks, which are associated with impaired energy generation, abnormal intestinal absorption, and regulation of actin cytoskeleton processes. These findings provide insights into the mechanisms of intestinal hypofunction induced by PAD.

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Severe pantothenic acid deficiency induces alterations in the intestinal mucosal proteome of starter Pekin ducks

10.21203/rs.3.rs-37755/v1 ◽

2020 ◽

Author(s):

Jing Tang ◽

Yulong Feng ◽

Bo Zhang ◽

Yongbao Wu ◽

Zhanbao Guo ◽

...

Keyword(s):

Oxidative Stress ◽

Actin Cytoskeleton ◽

Intestinal Absorption ◽

Pantothenic Acid ◽

Energy Generation ◽

Growth Depression ◽

Pekin Ducks ◽

Acid Deficiency ◽

Regulation Of Actin Cytoskeleton ◽

Pantothenic Acid Deficiency

Abstract Background Pantothenic acid deficiency (PAD) results in growth depression and intestinal hypofunction of animals. The underlying mechanisms, however, remain to be established and an overview of molecular alterations is still lacking. We investigated intestinal mucosal proteome changes induced by PAD in ducks to explain its effects on growth and intestine. Methods A total of 128 one-day-old Pekin ducks were divided into two groups, with 8 replicates and 8 birds per replicate. All the ducks were fed either a PAD or a pantothenic acid adequate (control, CON) diet for 16 days. Results High mortality, growth retardation, fasting hypoglycemia, reduced plasma insulin, and oxidative stress were observed in the PAD group compared to the CON group. Furthermore, PAD induced morphological alterations of the small intestine indicated by reduced villus height and villus surface area of duodenum, jejunum, and ileum. The duodenum mucosal proteome of ducks showed that 198 proteins were upregulated and 223 proteins were downregulated (> 1.5-fold change) in the PAD group compared to those in the CON group. Pathway analysis of these proteins exhibited the suppression of glycolysis and gluconeogenesis, fatty acid beta oxidation, tricarboxylic acid cycle, oxidative phosphorylation, oxidative stress, and intestinal absorption in the PAD group, indicating impaired energy generation and abnormal intestinal absorption. We also show that nine out of eleven proteins involved in regulation of actin cytoskeleton were upregulated by PAD, probably indicates reduced intestinal integrity. Conclusion PAD leads to growth depression and intestinal hypofunction of ducks, which are associated with impaired in energy generation, abnormal intestinal absorption, and regulation of actin cytoskeleton processes. These findings contribute to our understanding of the mechanisms of intestinal mucosa metabolic disorders due to PAD.

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Severe Pantothenic Acid Deficiency Induces Alterations in The Intestinal Mucosal Proteome of Starter Pekin Ducks

10.21203/rs.3.rs-46187/v1 ◽

2020 ◽

Author(s):

Jing Tang ◽

Yulong Feng ◽

Bo Zhang ◽

Yongbao Wu ◽

Zhanbao Guo ◽

...

Keyword(s):

Oxidative Stress ◽

Actin Cytoskeleton ◽

Intestinal Absorption ◽

Pantothenic Acid ◽

Energy Generation ◽

Growth Depression ◽

Pekin Ducks ◽

Acid Deficiency ◽

Regulation Of Actin Cytoskeleton ◽

Pantothenic Acid Deficiency

Abstract Background: Pantothenic acid deficiency (PAD) results in growth depression and intestinal hypofunction of animals. However, the underlying mechanisms remain to be established. We investigated intestinal mucosal proteome changes induced by PAD in ducks to explain its effects on growth and intestine. Results: A total of 128 one-day-old Pekin ducks were divided into two groups, with 8 replicates and 8 birds per replicate. All the ducks were fed either a PAD or a pantothenic acid adequate (control, CON) diet for 16 days. High mortality, growth retardation, fasting hypoglycemia, reduced plasma insulin, and oxidative stress were observed in the PAD group compared to the CON group. Furthermore, PAD induced morphological alterations of the small intestine indicated by reduced villus height and villus surface area of duodenum, jejunum, and ileum. The duodenum mucosal proteome of ducks showed that 198 proteins were upregulated and 223 proteins were downregulated (> 1.5-fold change) in the PAD group compared to those in the CON group. Pathway analysis of these proteins exhibited the suppression of glycolysis and gluconeogenesis, fatty acid beta oxidation, tricarboxylic acid cycle, oxidative phosphorylation, oxidative stress, and intestinal absorption in the PAD group, indicating impaired energy generation and abnormal intestinal absorption. We also show that nine out of eleven proteins involved in regulation of actin cytoskeleton were upregulated by PAD, probably indicates reduced intestinal integrity. Conclusion: PAD leads to growth depression and intestinal hypofunction of ducks, which are associated with impaired in energy generation, abnormal intestinal absorption, and regulation of actin cytoskeleton processes. These findings have important implications for understanding the effect of PAD on intestines.

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Severe Pantothenic Acid Deficiency Induces Alterations in the Intestinal Mucosal Proteome of Starter Pekin Ducks

10.21203/rs.3.rs-32667/v1 ◽

2020 ◽

Author(s):

Jing Tang ◽

Yulong Feng ◽

Bo Zhang ◽

Yongbao Wu ◽

Zhanbao Guo ◽

...

Keyword(s):

Intestinal Absorption ◽

Tricarboxylic Acid Cycle ◽

Pantothenic Acid ◽

Energy Generation ◽

Growth Depression ◽

Pekin Ducks ◽

Acid Deficiency ◽

Underlying Mechanisms ◽

Fasting Hypoglycemia ◽

Pantothenic Acid Deficiency

Abstract Background: Pantothenic acid deficiency (PAD) results in growth depression and intestinal hypofunction of animals. The underlying mechanisms, however, remain to be established and an overview of molecular alterationsis still lacking.We investigated intestinal mucosalproteome changes induced by PADin ducks to explain its effects on growth and intestine.Methods:A total of128 one-day-oldPekinducks were divided into two groups,with 8 replicates and 8 birds per replicate. All the ducks were fed either a PAD or a pantothenic acid adequate (control, CON) dietfor 16 days.Results:High mortality, growthretardation,fasting hypoglycemia,reduced plasma insulin, and oxidative stresswere observed in the PAD group compared to the CON group. Furthermore, PAD induced morphological alterationsof the small intestine indicated by reduced villus height and villus surface area of duodenum, jejunum, and ileum.The duodenum mucosal proteome of ducks showed that 198 proteins were upregulated and 223 proteins were downregulated (> 1.5-fold change) in the PAD group compared to those in the CON group. Pathway analysis of these proteins exhibited the suppression of glycolysis and gluconeogenesis, fatty acid beta oxidation, tricarboxylic acid cycle, oxidative phosphorylation, oxidative stress, and intestinal absorption in the PAD group, indicating impaired energy generation and abnormal intestinal absorption.Wealso show that nine out of eleven proteins involved in regulation of actin cytoskeleton were upregulated by PAD, probably indicates reduced intestinal integrity.Conclusion:PAD leads to growth depression and intestinal hypofunctionof ducks, which are associated with impaired in energy generation, abnormal intestinal absorption, and regulation of actin cytoskeletonprocesses. These findings contribute to our understanding of the mechanisms of intestinal mucosa metabolic disorders due to PAD.

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