scholarly journals Tannic acid-modified silver nanoparticles enhance anti-Acanthamoeba activity without increasing cytotoxicity of three multipurpose contact lens solutions

2020 ◽  
Author(s):  
Edyta Beata Hendiger ◽  
Marcin Padzik ◽  
Agnieszka Żochowska ◽  
Wanda Baltaza ◽  
Gabriela Olędzka ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Tannic Acid ◽  
Contact Lens ◽  
Contact Lenses ◽  
Colorimetric Assay ◽  
Statistical Significance ◽  
Plant Metabolites ◽  
Contact Lens Solutions ◽  
Preventive Actions

Abstract Background: Free living amoebae of Acanthamoeba genus are cosmopolitan, widely distributed protozoans causing severe, vision-threatening corneal infection known as Acanthamoeba keratitis (AK). Majority of the increasing number of AK cases are associated with contact lenses use. Due to lack of effective therapies against AK, proper eye hygiene and effective contact lenses disinfection are crucial in prevention of this infection. Currently available multipurpose contact lens disinfection systems are not fully effective against Acanthamoeba trophozoites and cysts. There is an urgent need to increase the disinfecting activity of these systems to prevent Acanthamoeba keratitis infections. Synthesized nanoparticles have been recently studied and proposed as a new generation of anti-microbial agents. It is also known that plant metabolites, including tannins, present anti-parasitic activity. The aim of this study was to evaluate the anti-amoebic activity and cytotoxicity of the tannic acid-modified silver nanoparticles (AgTANPs) conjugated with the selected multipurpose contact lens solutions.Methods: The anti-amoebic activity of pure contact lens care solutions and nanoparticles conjugated with contact lens care solutions were examined in vitro by colorimetric assay, based on the oxido-reduction of AlamarBlue. The cytotoxicity assays were performed using a fibroblast HS-5 (ATCC CRL-11882) cell line. The results were statistically analyzed by ANOVA and Student-Newman-Keuls tests using the p<0.05 level of a statistical significance.Results: The obtained results showed that nanoparticles enhanced anti-Acanthamoeba activity of the tested contact lens solutions without increasing their cytotoxicity profile. The activity is enhanced within minimal disinfection time recommended by the manufacturer.Conclusions: The conjugation of the selected contact lens solutions with AgTANPs might be a novel and promising approach as a part of preventive actions of Acanthamoeba keratitis infections among contact lens users.

scholarly journals Tannic acid-modified silver nanoparticles enhance anti-Acanthamoeba activity without increasing cytotoxicity of three multipurpose contact lens solutions

2020 ◽  
Author(s):  
Edyta Beata Hendiger ◽  
Marcin Padzik ◽  
Agnieszka Żochowska ◽  
Wanda Baltaza ◽  
Gabriela Olędzka ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Tannic Acid ◽  
Contact Lens ◽  
Contact Lenses ◽  
Colorimetric Assay ◽  
Statistical Significance ◽  
Plant Metabolites ◽  
Contact Lens Solutions ◽  
Preventive Actions

Abstract Background: Free living amoebae of the genus Acanthamoeba are cosmopolitan, widely distributed protozoans causing a severe, vision-threatening corneal infection known as Acanthamoeba keratitis (AK). The majority of the increasing number of AK cases are associated with contact lenses use. Appropriate eye hygiene and effective contact lenses disinfection are crucial in prevention of this infection because of the lack of effective therapies against AK. Currently available multipurpose contact lens disinfection systems are not fully effective against Acanthamoeba trophozoites and cysts. There is an urgent need to increase the disinfecting activity of these systems to prevent Acanthamoeba keratitis infections. Synthesized nanoparticles have been recently studied and proposed as a new generation of anti-microbial agents. It is also known that plant metabolites, including tannins, present anti-parasitic activity. The aim of this study was to evaluate the anti-amoebic activity and cytotoxicity of the tannic acid-modified silver nanoparticles (AgTANPs) conjugated with the selected multipurpose contact lens solutions.Methods: The anti-amoebic activity of pure contact lens care solutions and nanoparticles conjugated with contact lens care solutions were examined in vitro by a colorimetric assay, based on the oxido-reduction of AlamarBlue. The cytotoxicity assays were performed using a fibroblast HS-5 (ATCC CRL-11882) cell line. The results were statistically analyzed by ANOVA and Student-Newman-Keuls tests using the p<0.05 level of a statistical significance.Results: We show that nanoparticles enhanced anti-Acanthamoeba activity of the tested contact lens solutions without increasing their cytotoxicity profile. The activity is enhanced within the minimal disinfection time recommended by the manufacturer.Conclusions: The conjugation of the selected contact lens solutions with AgTANPs might be a novel and promising approach as a part of preventive actions of Acanthamoeba keratitis infections among contact lens users.

scholarly journals Tannic acid-modified silver nanoparticles enhance anti-Acanthamoeba activity without increasing cytotoxicity of three multipurpose contact lens solutions

2020 ◽  
Author(s):  
Edyta Beata Hendiger ◽  
Marcin Padzik ◽  
Agnieszka Żochowska ◽  
Wanda Baltaza ◽  
Gabriela Olędzka ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Tannic Acid ◽  
Contact Lens ◽  
Contact Lenses ◽  
Colorimetric Assay ◽  
Statistical Significance ◽  
Plant Metabolites ◽  
Contact Lens Solutions ◽  
Preventive Actions

Abstract Background: Free living amoebae of the genus Acanthamoeba are cosmopolitan, widely distributed protozoans causing a severe, vision-threatening corneal infection known as Acanthamoeba keratitis (AK). The majority of the increasing number of AK cases are associated with contact lenses use. Appropriate eye hygiene and effective contact lenses disinfection are crucial in prevention of this infection because of the lack of effective therapies against AK. Currently available multipurpose contact lens disinfection systems are not fully effective against Acanthamoeba trophozoites and cysts. There is an urgent need to increase the disinfecting activity of these systems to prevent Acanthamoeba keratitis infections. Synthesized nanoparticles have been recently studied and proposed as a new generation of anti-microbial agents. It is also known that plant metabolites, including tannins, present anti-parasitic activity. The aim of this study was to evaluate the anti-amoebic activity and cytotoxicity of the tannic acid-modified silver nanoparticles (AgTANPs) conjugated with the selected multipurpose contact lens solutions.Methods: The anti-amoebic activity of pure contact lens care solutions and nanoparticles conjugated with contact lens care solutions were examined in vitro by a colorimetric assay, based on the oxido-reduction of AlamarBlue. The cytotoxicity assays were performed using a fibroblast HS-5 (ATCC CRL-11882) cell line. The results were statistically analyzed by ANOVA and Student-Newman-Keuls tests using the P < 0.05 level of a statistical significance.Results: We show that nanoparticles enhanced anti-Acanthamoeba activity of the tested contact lens solutions without increasing their cytotoxicity profile. The activity is enhanced within the minimal disinfection time recommended by the manufacturer.Conclusions: The conjugation of the selected contact lens solutions with AgTANPs might be a novel and promising approach as a part of preventive actions of Acanthamoeba keratitis infections among contact lens users.

scholarly journals Tannic acid-modified silver nanoparticles enhance anti-Acanthamoeba activity without increasing cytotoxicity of the contact lens solutions

2020 ◽  
Author(s):  
Edyta Beata Hendiger ◽  
Marcin Padzik ◽  
Agnieszka Żochowska ◽  
Wanda Baltaza ◽  
Gabriela Olędzka ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Tannic Acid ◽  
Contact Lens ◽  
Contact Lenses ◽  
Colorimetric Assay ◽  
Statistical Significance ◽  
Acanthamoeba Keratitis ◽  
Plant Metabolites ◽  
Contact Lens Solutions

Abstract Background: Free living amoebae of Acanthamoeba genus are cosmopolitan, widely distributed protozoans causing severe, vision-threatening corneal infection known as Acanthamoeba keratitis (AK). Majority of the increasing number of AK cases are associated with contact lenses use. Due to lack of effective therapies against AK, proper eye hygiene and effective contact lenses disinfection are crucial in prevention of this infection. Currently available multipurpose contact lens disinfection systems are not fully effective against Acanthamoeba trophozoites and cysts. There is an urgent need to increase the disinfecting activity of these systems to prevent Acanthamoeba keratitis infections. Synthesized nanoparticles have been recently studied and proposed as a new generation of anti-microbial agents. It is also known that plant metabolites, including tannins, present anti-parasitic activity. The aim of this study was to evaluate the anti-amoebic activity and cytotoxicity of the tannic acid-modified silver nanoparticles (AgTANPs) conjugated with selected multipurpose contact lens solutions. Methods: The anti-amoebic activity of pure contact lens care solutions and nanoparticles conjugated with contact lens care solutions were examined in vitro by colorimetric assay, based on the oxido-reduction of AlamarBlue. The cytotoxicity assays were performed using a fibroblast HS-5 (ATCC CRL-11882) cell line. Results were statistically analyzed by ANOVA and Student-Newman-Keuls tests using the p<0.05 level of statistical significance. Results: The obtained results showed that nanoparticles enhanced anti-Acanthamoeba activity of the tested contact lens solutions without increasing their cytotoxicity profile. The activity is enhanced within minimal disinfection time recommended by the manufacturer. Conclusions: The conjugation of selected contact lens solutions with AgTANPs might be a novel and promising approach as part of preventive actions of Acanthamoeba keratitis infections among contact lens users.

scholarly journals Tannic acid-modified silver nanoparticles enhance the anti-Acanthamoeba activity of three multipurpose contact lens solutions without increasing their cytotoxicity

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Edyta B. Hendiger ◽  
Marcin Padzik ◽  
Agnieszka Żochowska ◽  
Wanda Baltaza ◽  
Gabriela Olędzka ◽  
...  
Keyword(s):  
Tannic Acid ◽  
Contact Lens ◽  
Colorimetric Assay ◽  
Statistical Significance ◽  
Plant Metabolites ◽  
Contact Lens Solutions ◽  
Effective Contact ◽  
Silver Nps ◽  
New Generation

Abstract Background Free-living amoebae of the genus Acanthamoeba are cosmopolitan, widely distributed protozoans that cause a severe, vision-threatening corneal infection known as Acanthamoeba keratitis (AK). The majority of the increasing number of AK cases are associated with contact lens use. Appropriate eye hygiene and effective contact lens disinfection are crucial in the prevention of AK because of the lack of effective therapies against it. Currently available multipurpose contact lens disinfection systems are not fully effective against Acanthamoeba trophozoites and cysts. There is an urgent need to increase the disinfecting activity of these systems to prevent AK infections. Synthesized nanoparticles (NPs) have been recently studied and proposed as a new generation of anti-microbial agents. It is also known that some plant metabolites, including tannins, have anti-parasitic activity. The aim of this study was to evaluate the anti-amoebic activity and cytotoxicity of tannic acid-modified silver NPs (AgTANPs) conjugated with selected multipurpose contact lens solutions. Methods The anti-amoebic activities of pure contact lens care solutions, and NPs conjugated with contact lens care solutions, were examined in vitro by a colorimetric assay based on the oxido-reduction of alamarBlue. The cytotoxicity assays were performed using a fibroblast HS-5 (ATCC CRL-11882) cell line. The results were statistically analysed by ANOVA and Student-Newman-Keuls test using P < 0.05 as the level of statistical significance. Results We show that the NPs enhance the anti-Acanthamoeba activities of the tested contact lens solutions without increasing their cytotoxicity profiles. The activities are enhanced within the minimal disinfection time recommended by the manufacturers. Conclusions The conjugation of the selected contact lens solutions with AgTANPs might be a novel and promising approach for the prevention of AK infections among contact lens users.

In Vitro Efficacy of Contact Lens Solutions Against Various Corneal Fungal Isolates

2013 ◽  
Vol 29 (7) ◽  
pp. 694-699 ◽  
Author(s):  
Antonio Pinna ◽  
Donatella Usai ◽  
Stefania Zanetti ◽  
Philip A. Thomas ◽  
Jayaraman Kaliamurthy
Keyword(s):  
Contact Lens ◽  
Fungal Isolates ◽  
Contact Lens Solutions

In vitro efficiency of contact lens care solutions in removing cholesterol deposits from silicone hydrogel contact lenses

2013 ◽  
Vol 36 ◽  
pp. e41
Author(s):  
Lakshman Subbaraman ◽  
Hendrik Walther ◽  
Lise Kay ◽  
Lyndon Jones
Keyword(s):  
Contact Lens ◽  
Contact Lenses ◽  
Silicone Hydrogel

scholarly journals The Bio-Tribological Effect of Poly-Gamma-Glutamic Acid in the Lysozyme-Ionic Contact Lens System

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 156
Author(s):  
Chen-Ying Su ◽  
Lung-Kun Yeh ◽  
Chi-Chun Lai ◽  
Mihaela Dubuisson ◽  
Yi-Fei Tsao ◽  
...  
Keyword(s):  
Friction Coefficient ◽  
Glutamic Acid ◽  
Contact Lens ◽  
Contact Lenses ◽  
Lens System ◽  
Friction Testing ◽  
Lubricating Property ◽  
Velocity Variations

Feeling comfortable is an important issue for contact lens wearers as contact lenses are worn for an extensive period of time. It has been shown that the in vitro friction coefficient of contact lenses is correlated to the degree of in vivo comfort, thus many studies focus on establishing friction testing methods for investigating the friction coefficient of contact lenses or contact lens care solution. We have previously demonstrated the lubricating property of poly-gamma-glutamic acid (γ-PGA)-containing care solution, and it could reduce the high friction coefficient caused by lysozyme. However, the mechanism of how γ-PGA-containing care solution reduces the lysozyme-induced friction coefficient of contact lenses is unclear. We investigated the bio-tribological effect of γ-PGA on ionic contact lenses in the presence of lysozyme by testing load and velocity variations. The ability to remove lysozyme deposition by γ-PGA and viscosity analysis of γ-PGA-containing care solutions were also investigated to understand the potential mechanism. Our results showed that the friction coefficient of γ-PGA-containing care solution with lysozyme was the lowest in both load and velocity variations, and γ-PGA functions distinctly in the lysozyme-ionic contact lens system. We proposed a model of how γ-PGA could reduce the friction coefficient in these two conditions.

scholarly journals Extended levobunolol release from Eudragit nanoparticle-laden contact lenses for glaucoma therapy

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Navneet Kumar ◽  
Rohan Aggarwal ◽  
Meenakshi K. Chauhan
Keyword(s):  
Particle Size ◽  
Drug Release ◽  
Zeta Potential ◽  
Contact Lens ◽  
Contact Lenses ◽  
Ex Vivo ◽  
Eye Drops ◽  
Equilibrium Swelling ◽  
Drug Solution

Abstract Background Majorly, the reason for the permanent loss of vision is glaucoma. But the currently available common treatment methodologies such as eye drops have various disadvantages like patient incompliance due to repeated administration and poor (1–5%) bioavailability leading to poor efficiency. The objective of this research was to formulate Eudragit-based nanoparticles of levobunolol incorporated into a contact lens to obtain sustained ocular delivery of levobunolol at the therapeutics level. Eudragit nanoparticles of levobunolol were formulated by nanoprecipitation methodology utilizing different ratios of Eudragit S100 and polyvinyl alcohol. The prepared nanoparticles were evaluated and optimized by efficiency of entrapment, particle size, morphology of surface and zeta potential. The optimized nanoparticles were then entrapped into the matrix of the contact lens by the soaking method which were then characterized and compared for optical clarity study, equilibrium swelling study, shelf life and in vitro drug release in simulated tear fluid followed by ex vivo transcorneal permeation study. Results Formulation F3 was obtained as optimized nanoparticle formulation with 102.61 nm ± 3.92 of particle size, − 22.2 mV ± 2.76 of zeta potential and 86.995% ± 1.902 of efficiency of entrapment. The equilibrium swelling index and transmittance of nanoparticle incorporated into contact lenses showed better results when compared to drug solution-loaded lenses. In vitro release indicated more sustained drug profiles (84.33% ± 0.34 of drug release over a period of 12 days) as compared to drug solution-loaded lenses (89.282% ± 0.900 of drug release over a period of 3 days). Ex vivo transcorneal permeation studies showed more permeation (6.75% ± 0.170) through contact lenses as compared to marketed eye drops (3.03% ± 0.088). Conclusion This research demonstrates the remarkable results of drug-laden contact lenses to serve as a great medium for the continued delivery of ocular drugs without affecting the physical and optical characteristics of the lens content.

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