How Does Nuclear Organization Maintain Normal Mammary Phenotype

2002 ◽  
Author(s):  
Sophie A. Lelievre
Keyword(s):  
Nuclear Organization

Author response for "Amphibian thalamic nuclear organization during larval development and in the adult frog Xenopus laevis : genoarchitecture and hodological analysis"

2020 ◽  
Author(s):  
Ruth Morona ◽  
Sandra Bandín ◽  
Jesús M. López ◽  
Nerea Moreno ◽  
Agustín González
Keyword(s):  
Xenopus Laevis ◽  
Larval Development ◽  
Nuclear Organization ◽  
Author Response ◽  
Adult Frog

Quantitative Model-Based Image Analysis of NuMa Distribution Links Nuclear Organization with Cell Phenotype

2001 ◽  
Vol 7 (S2) ◽  
pp. 578-579
Author(s):  
David W. Knowles ◽  
Sophie A. Lelièvre ◽  
Carlos Ortiz de Solόrzano ◽  
Stephen J. Lockett ◽  
Mina J. Bissell ◽  
...  
Keyword(s):  
Image Analysis ◽  
Mammary Epithelial Cells ◽  
Nuclear Organization ◽  
Critical Role ◽  
Quantitative Model ◽  
Mammary Epithelial ◽  
Cell Phenotype ◽  
Proliferating Cells ◽  
Mitotic Apparatus ◽  
Model Based

The extracellular matrix (ECM) plays a critical role in directing cell behaviour and morphogenesis by regulating gene expression and nuclear organization. Using non-malignant (S1) human mammary epithelial cells (HMECs), it was previously shown that ECM-induced morphogenesis is accompanied by the redistribution of nuclear mitotic apparatus (NuMA) protein from a diffuse pattern in proliferating cells, to a multi-focal pattern as HMECs growth arrested and completed morphogenesis . A process taking 10 to 14 days.To further investigate the link between NuMA distribution and the growth stage of HMECs, we have investigated the distribution of NuMA in non-malignant S1 cells and their malignant, T4, counter-part using a novel model-based image analysis technique. This technique, based on a multi-scale Gaussian blur analysis (Figure 1), quantifies the size of punctate features in an image. Cells were cultured in the presence and absence of a reconstituted basement membrane (rBM) and imaged in 3D using confocal microscopy, for fluorescently labeled monoclonal antibodies to NuMA (fαNuMA) and fluorescently labeled total DNA.

scholarly journals Protocol for using TRIBE to study RNA-protein interactions and nuclear organization in mammalian cells

2021 ◽  
Vol 2 (3) ◽  
pp. 100634
Author(s):  
Jeetayu Biswas ◽  
Michael Rosbash ◽  
Robert H. Singer ◽  
Reazur Rahman
Keyword(s):  
Protein Interactions ◽  
Mammalian Cells ◽  
Nuclear Organization

scholarly journals Nuclear Physics: Quantitative Single-Cell Approaches to Nuclear Organization and Gene Expression

2010 ◽  
Vol 75 (0) ◽  
pp. 113-126 ◽  
Author(s):  
T. Lionnet ◽  
B. Wu ◽  
D. Grunwald ◽  
R. H. Singer ◽  
D. R. Larson
Keyword(s):  
Gene Expression ◽  
Single Cell ◽  
Nuclear Physics ◽  
Nuclear Organization

scholarly journals Nuclear organization in differentiating oligodendrocytes

2002 ◽  
Vol 115 (21) ◽  
pp. 4071-4079 ◽  
Author(s):  
J. A. Nielsen
Keyword(s):  
Nuclear Organization

Nuclear organization of orexinergic neurons in the hypothalamus of a lar gibbon and a chimpanzee

2021 ◽  
Author(s):  
Victoria M. Williams ◽  
Adhil Bhagwandin ◽  
Jordan Swiegers ◽  
Mads F. Bertelsen ◽  
Therese Hård ◽  
...  
Keyword(s):  
Nuclear Organization
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