scholarly journals Callus Induction and Plantlet Regeneration in Tissue Cultures of Hawaiian Anthuriums

HortScience ◽  
1991 ◽  
Vol 26 (7) ◽  
pp. 919-921 ◽  
Author(s):  
A.R. Kuehnle ◽  
N. Sugii
Keyword(s):  
Acetic Acid ◽  
Callus Induction ◽  
Leaf Explants ◽  
Plantlet Regeneration ◽  
Tissue Cultures ◽  
Petiole Explants ◽  
Anthurium Andraeanum ◽  
Dichlorophenoxy Acetic Acid

Leaf explants of seven cultivars of Hawaiian anthuriums (Anthurium andraeanum Linden ex André cv. Kaumana, Kozohara, Marian Seefurth, Mauna Kea, Nitta, Ozaki, and Paradise Pink) produced callus most successfully after 2 to 3 months on a modified Pierik medium containing 0.36 μm 2,4-D and 4.4 μm BA. Petiole explants callused best on Pierik modified Pierik, and Finnie and van Staden media. Long-term cultures of callus from Univ. of Hawaii anthurium selections UH965, UH1060, and UH1003 were maintained for 12 to 13 months and were still capable of plantlet regeneration. Adventitious plantlets were recovered from callus plated on a Kunisaki medium containing 2.2 or 22 μm BA. Regeneration appeared to be organogenic rather than embryogenic and varied among the genotypes tested. Chemical names used: N- (phenylmethyl)-1H-purin-6-amine (BA); (2,4-dichlorophenoxy) acetic acid (2,4-D).

scholarly journals Sterilization procedure and callus regeneration in black turmeric (Curcuma caesia)

2019 ◽  
Vol 6 (49) ◽  
Author(s):  
A. S. Abubakar ◽  
R. N. Pudake
Keyword(s):  
Acetic Acid ◽  
Callus Induction ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Surface Sterilization ◽  
To Come ◽  
Dichlorophenoxy Acetic Acid ◽  
And Control ◽  
Rhizome Explants

Sterilization procedure, media composition, explants selection and control of physical environment are critical for successful cultures and callus induction with surface sterilization being very challenging in most plants. Five different sterilization methods were evaluated to come up with the best for subsequent use to establish an in vitro regeneration method for the induction of callus in Curcuma caesia using excised leaf and rhizome explants. Murashige and Skoog (MS) media supplemented with various concentration of 2,4-Dichlorophenoxy acetic acid (2,4-D)/Indole-3-acetic acid (IAA) (0.5- 5.0mg/L), singly or in combination with Benzyl aminopurine (BAP)/Kinetin (KIN) (0.1-5.0mg/L), 0.3% sucrose and 0.08% agar were used. The result of the sterilization procedures showed 15% NaHClO3 (5min) + 70% Ethanol (30s) + 0.1% HgCl2 (5min) to be the most effective in controlling contamination in C. caesia among all the treatments tested. The response to callus induction was found to depend on the type of explants used and growth regulators combination. Leaf explants gave the highest percentage of callus induction. Highest percentage of callus induction (66.70%) was obtained in the growth regulator combination of 2, 4-D (0.5mg/L) + BAP (0.1mg/L) and least (14.29%) in IAA (2.0mg/L) + BAP (0.5mg/L). Equal and higher concentration of 2, 4-D + BAP of 5.0mg/L each also provided better result (40.00%). No callus was obtained in all the single concentration of 2, 4-D used.

scholarly journals Effect of phytohormones on shoot apex and leaf explants of Withania somnifera (Ashwagandha)

2016 ◽  
Vol 8 (1) ◽  
pp. 412-415 ◽  
Author(s):  
Archana Rani ◽  
M. Kumar ◽  
Sanjeev Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Apex ◽  
Callus Induction ◽  
Withania Somnifera ◽  
Leaf Explants ◽  
Ms Medium ◽  
Mass Propagation ◽  
Best Response ◽  
Dichlorophenoxy Acetic Acid

An efficient protocol for callus induction of Withania somnifera through in vitro culture of shoot apex and leaf explant was standardized. Of the various combinations of phytohormones evaluated, MS media supplemented with 6-furfuryl aminopurine (KIN) 0.5 mg/l + 2,4-dichlorophenoxy acetic acid (2, 4-D) 2.0 mg/l was found to be bestfor mean callus induction (86%) in leaf explants after 6 weeks of culture and in case of shoot apex expant the best response and growth of callusing was observed on MS medium supplemented with 2,4-D 1.0 mg/l + BAP 2.0 mg/l (77%).The response of callus growth increases gradually with the reductions in concentration of KIN in culturemedium of both the explants. This protocol might be used in further research for mass propagation of W. somnifera via indirect regeneration methods.

scholarly journals Micropropagation, Callus Induction and Regeneration of Ginger (Zingiber officinale Rosc.)

2020 ◽  
Vol 5 (1) ◽  
pp. 75-84
Author(s):  
Seied Mehdi Miri
Keyword(s):  
Acetic Acid ◽  
Callus Induction ◽  
Leaf Explants ◽  
Multiple Shoots ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Shoot Number ◽  
Micro Propagation ◽  
Dichlorophenoxy Acetic Acid

AbstractThe present study describes a protocol for micro-propagation, callus induction, and shoot regeneration of ginger (Zingiber officinale). The rhizomes were surface-sterilized with ethanol (70%) for 45 s, sodium hypochlorite (2.5%) for 10 min, and mercuric chloride (0.1%) for 10 min. Multiple shoots were induced from sprouting bud explants cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA) combined with kinetin (Kin). The maximum shoot number was obtained from MS medium containing 10 mg/l BA with a mean of 20.6 shoots per explant. The leaf explants were cultured on MS medium supplemented with indole-3-acetic acid (IAA), naphthaleneacetic acid (NAA), 2,4-dichlorophenoxy acetic acid (2,4-D), Dicamba, or BA for callus culture. Green-red compact calli were induced using 2,4-D, Dicamba or BA. Also, BA successfully induced plant regeneration. The multiplied shoots that were transferred to the rooting medium (½MS supplemented with 0, 1 and 2 mg/l IAA, indole-3-butyric acid (IBA) or NAA) showed development of roots (100%). The rooted plantlets were transferred to pots containing a 1:1 mixture of cocopeat and perlite, and acclimatization was successful, resulting in 85% survival of the plantlets in the greenhouse.

In vitro plantlet formation from flower petal explants of Hemerocallis cv. Chipper Cherry

1976 ◽  
Vol 54 (7) ◽  
pp. 616-618 ◽  
Author(s):  
Charles W. Heuser ◽  
Darrel A. Apps
Keyword(s):  
Acetic Acid ◽  
Callus Tissue ◽  
Callus Cultures ◽  
Plantlet Regeneration ◽  
Tissue Cultures ◽  
Flower Petal ◽  
Plantlet Formation ◽  
Yellowish Green ◽  
Dichlorophenoxy Acetic Acid

Plantlet regeneration has been induced from callus tissue cultures obtained from petal parts of Hemerocallis cv. Chipper Cherry. Callus cultures capable of regenerating whole plantlets were established on the agar-solidified Murashige and Skoog's medium supplemented with (2,4-dichlorophenoxy)acetic acid (2,4-D) (1.0 mg/litre) + kinetin (1.0 mg/litre). The callus formed was dense, yellowish-green in color, and had what appeared to be meristematic protuberances. Shoots and roots developed when the callus was subcultured on a medium lacking 2,4-D.

scholarly journals Role of Growth Regulators on In Vitro Callus Induction and Direct Regeneration in Physalis minima Linn

2015 ◽  
Vol 44 ◽  
pp. 38-44 ◽  
Author(s):  
H. Sandhya ◽  
Rao Srinath
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Basal Medium ◽  
Direct Regeneration ◽  
Naphthalene Acetic Acid ◽  
Stem Explants ◽  
Dichlorophenoxy Acetic Acid ◽  
Indole 3 Acetic Acid

Suitable protocol for induction of callus and regeneration was developed from different explants viz., node, stem and leaves in Physalis minima. MS basal medium supplemented with various concentrations (1.0-4.0mg/l) of auxins like 2,4-Dichlorophenoxy acetic acid (2,4-D), α-naphthalene acetic acid (NAA) and Indole-3-acetic acid (IAA) and cytokinins (0.5-1.5mg/l) like BAP or Kn were used. All the three explants responded for induction of callus, however stem explants were found superior, followed by node and leaf. Callus induction was observed in all the auxins and combination of growth regulators used with varied mass (2010±1.10) and highest percentage of callus induction was observed from stem at 2.0mg/l 2,4-D (90%) followed by NAA (70%) and IAA (50%). Organogenesis was induced when nodal explants were transferred on MS medium supplemented with 2,4-D and Kn at various concentrations, maximum being on 2.0mg/l 2,4-D + 1.0mg/l Kn (90%). Regenerated shoots were elongated on 0.5mg/l GA3. The shoots were subsequently rooted on MS + 1.0mg/l IBA (95%) medium. Rooted shoots were hardened and acclimatized, later they were transferred to polycups containing soil, cocopeat and sand in the ratio 1:2:1.Keywords:Physalis minima, Node, Stem, Leaf, callus and growth regulators.

scholarly journals In vitro callus induction of gerbera from leaf explant

2020 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Sadia Afrin Jui ◽  
Md. Mijanur Rahman Rajib ◽  
M. Mofazzal Hossain ◽  
Sharmila Rani Mallik ◽  
Iffat Jahan Nur ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Leaf Explants ◽  
Leaf Explant ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Indole 3 Acetic Acid

The experiment was designed to evaluate the effect of growth regulators on leaf explant of Gerbera for callus induction. Various kinds of plant growth regulators such as 6-Benzylaminopurine (BAP), α-Naphthalene acetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Indole-3-acetic acid (IAA) were used to initiate cultures. These were added to Murashige and Skoog medium in different combinations and concentrations. Leaf explants cultured on MS medium supplemented with BAP+ 2, 4-D+ IAA in T4 treatment & BAP+ 2,4-D in T5 treatment showed the best results for callus induction. On the other hand callus was induced early in the combination of BA+ 2,4-D + IAA hormone in T5, T9 & T8 treatment respectively. The rate of callus induction was very low in BA + NAA combinations but it was much earlier.   

scholarly journals Plant Regeneration from Hypocotyl Callus of Radish

HortScience ◽  
1990 ◽  
Vol 25 (10) ◽  
pp. 1286-1288 ◽  
Author(s):  
Sachiko Matsubara ◽  
Hegazi H. Hegazi
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Nicotinic Acid ◽  
Raphanus Sativus ◽  
Basal Medium ◽  
Plantlet Regeneration ◽  
Inorganic Salts ◽  
Callus Initiation ◽  
Dichlorophenoxy Acetic Acid

Callus initiation and growth and plantlet regeneration were studied using eight cultivars of Raphanus sativus L., including six Japanese radishes, one Chinese and one small `Comet' radish. The basal medium was composed of Murashige and Skoog inorganic salts, 2.0 mg myo-inositol/liter, 0.5 mg each of nicotinic acid and pyridoxine·HCl/liter, and 0.1 mg thiamine·HCl/liter, 30 g sucrose and 2 g Gelrite/liter. High callus yields were obtained on basal medium containing (mg·liter-1) 0.1 2,4-D and 1.0 BA for two Japanese radishes and 0.1 NAA and 1.0 kinetin for `Comet' radish. Shoots were regenerated from callus by subculturing on basal medium containing 0.1 or 1.0 mg BA/liter and then transferring to basal medium. Rooting occurred on basal medium. Although callus was obtained in all eight cultivars, shoots and plantlets were regenerated only from `Moriguchi', `Nerima Shirinaga', and `Comet'. Chemical names used: 2-(l-naphthyl) acetic acid (NAA); N-(phenylmethyl)-lH-purine-6-amine (BA); 2,4-dichlorophenoxy acetic acid (2,4-D); 6-(furfurylamino)purine (kinetin).

scholarly journals Callus Induction and Plantlet Regeneration From Centalla Asiatica Linn

2004 ◽  
Vol 2 (2) ◽  
pp. 81-85
Author(s):  
Indumathy A. ◽  
Mahalakshmi P. ◽  
C. R. Bojan
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Leaf Explants ◽  
Centella Asiatica ◽  
Plantlet Regeneration ◽  
Nodal Segments ◽  
Ms Medium ◽  
Root Proliferation ◽  
Mass Propagation

Efficient plant regeneration could be obtained from the derooted nodal segments of Centella asiatica with stole buds as the explant, when cultured on MS medium supplemented with BAP (10 ppm)+NAA(2ppm). Both callus and regeneration occurred simultaneously on the same medium. Profuse rooting were obtain on MS medium fortified with NAA (2ppm) from leaf explants. Shoot and root proliferation were observed on the medium supplemented with BAP(5 ppm)and NAA (2 ppm) through subculture. Mass propagation of plantlets were obtained through invitro culture.

scholarly journals Micropropagation of Liriope muscari via leaf explant

1969 ◽  
Vol 83 (3-4) ◽  
pp. 169-173
Author(s):  
Keithley L. Amory ◽  
John M. Gill
Keyword(s):  
Acetic Acid ◽  
Mass Production ◽  
In Vitro Propagation ◽  
Solid Medium ◽  
Leaf Explants ◽  
Ms Medium ◽  
Isopentenyl Adenine ◽  
Young Leaves ◽  
Dichlorophenoxy Acetic Acid

Young leaves of Liriope muscari provide an ample source of explants for in vitro propagation in tropical countries where flowering is scarce. Leaves were induced to form calli on a solid medium containing Murashige and Skoog (MS) salts and vitamins, 3% sucrose, 0.7% agar, 1 mg/L 2,4-dichlorophenoxy- acetic acid (2, 4-D) and 1 mg/L 6-furfurylaminopurine (kinetin). Only the proximal segments of the leaves produced calli. These calli were induced to produce multiple plantlets on MS medium, 3% sucrose, 0.7% agar, and 10 mg/L N6 (2-isopentenyl) adenine (2 ip). It is possible to use leaf explants for in vitro mass production of Liriope. However, in variegated varieties, only green or white plants were produced, because of a chimera in the original tissue.

Revegetation Following Massive Application of Selected Herbicides

Weed Science ◽  
1973 ◽  
Vol 21 (5) ◽  
pp. 409-412 ◽  
Author(s):  
A. R. Isensee ◽  
W. C. Shaw ◽  
W. A. Gentner ◽  
C. R. Swanson ◽  
B. C. Turner ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Chemical Analysis ◽  
Sodium Arsenite ◽  
Sodium Chlorate ◽  
Mechanical Methods ◽  
Untreated Check ◽  
Dichlorophenoxy Acetic Acid ◽  
Residual Control ◽  
Term Response

This investigation was initiated in 1954 at Beltsville, Maryland, to determine the initial and long-term response of vegetation to, and persistence of, massive quantities of herbicides. Vegetative responses were determined 1, 2, 3, and 15 years after treatment. Residual phytotoxicity and herbicide residues were determined 14 years after treatment with bioassay and chemical analysis. After 3 years, revegetation was nearly complete in plots treated with massive (up to 400 times recommended agricultural rates) quantities of fenuron (1,1-dimethyl-3-phenylurea), monuron [3-(p-chlorophenyl)-1,1-dimethylurea], dalapon (2,2-dichloropropionic acid), chlorpropham (isopropyl m-chlorocarbanilate), sodium chlorate, 2,4-D [(2,4-dichlorophenoxy)acetic acid], borax, sodium chlorate plus borax, and 2,4-D plus borax. Only diuron [3-(3,4-di chlorophenyl)-1,1-dimethylurea], DMU [3-(3,4-dichlorophenyl)-1-methylurea] sodium arsenite, and sodium arsenite plus sodium chlorate gave residual control of vegetation for more than 3 years. Revegetated plots were identical to untreated check plots whether the vegetation was initially killed by chemical or mechanical methods. Phytotoxic soil residues of DMU, diuron, and arsenate were present 14 years after application.

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