scholarly journals MOLECULAR CHARACTERIZATION OF A POLYGALACTURONASE INHIBITOR OF PEAR FRUIT

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 580c-580
Author(s):  
Henrik Stotz ◽  
Ann Powell ◽  
Susan Damon ◽  
Audrey Hentzen ◽  
Carl Greve ◽  
...  
Keyword(s):  
Blot Analysis ◽  
Cross Reactivity ◽  
Sequence Information ◽  
Pear Fruit ◽  
Higher Plant ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Glycosylation Sites ◽  
Polymerase Chain

Higher plant inhibitors of fungal polygalacturonases are potential contributors to plant defense. To test this hypothesis we have raised antibodies against the `Bartlett' pear fruit polygalacturonase inhibitor (PGIP) and cloned a pear fruit PGIP cDNA. The pear PGIP cDNA was isolated by polymerase chain reactions based on our amino acid and nucleotide sequence information. Sequence analysis predicts a gene product of 34.5 kD with an isoelectric point of 6.02 in agreement with our biochemical data. Seven potential glycosylation sites are consistent with the glycoprotein character of these PGIPs. Southern blot analysis suggests the presence of 1 or 2 genes in the pear genome. Northern blot analysis indicates the presence of a transcript of 1.5 kb. Western blot analysis shows cross-reactivity of the anti-pear PGIP antibody to various dicot species as well as corn.

scholarly journals Genetic polymorphism at the CSN1S1 gene in two Czech goat breeds

2008 ◽  
Vol 52 (No. 7) ◽  
pp. 199-202 ◽  
Author(s):  
Z. Sztankóová ◽  
V. Mátlová ◽  
G. Malá
Keyword(s):  
Genetic Polymorphism ◽  
Genetic Characterization ◽  
Polymorphic Information Content ◽  
Population Differences ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Hardy Weinberg Equilibrium ◽  
Polymerase Chain ◽  
I Gene

The genetic polymorphism of the <i>CSN1S1</i> (casein <i>alpha-S1</i>) locus was investigated in two endangered Czech goat breeds (White Shorthair and Brown Shorthair). These breeds are kept mainly for their good dairy performance. Genetic characterization of the <i>CSN1S1</i> locus contributes to the knowledge of the genetic structure of these two endangered breeds. The study was performed on 498 goats (333 White and 165 Brown Shorthair goats) by means of different polymerase chain reactions (PCR). We detected <i>A</i>* (associated with normal content of protein), <i>E</i>, <i>F</i> and <i>01</i> alleles. The analysis showed a prevalence of <i>CSN1S1 F</i> (0.658; 0.597) and <i>CSN1S1 A</i>* (0.269; 0.303) alleles. In both breeds, the frequency of occurrence of <i>E</i> and <i>01</i> alleles was very low: <i>E</i> (0.054; 0.085) and <i>01</i> (0.019; 0.015), respectively. No population followed the Hardy-Weinberg equilibrium, the value of polymorphic information content (PIC) being 0.426 in White and 0.472 in Brown Shorthair goats. Moreover, the test of population differences (<i>P</i> = 0.130) showed no significant differences between White and Brown Shorthair goats. This genetic peculiarity makes the preservation of the population of both breeds worthwhile.

scholarly journals Investigation of Lethal Concurrent Outbreak of Chlamydiosis and Pigeon Circovirus in a Zoo

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1654
Author(s):  
Wei-Tao Chen ◽  
Chin-Ann Teng ◽  
Cheng-Hsin Shih ◽  
Wei-Hsiang Huang ◽  
Yi-Fan Jiang ◽  
...  
Keyword(s):  
Disease Outbreaks ◽  
Chlamydia Psittaci ◽  
Bursa Of Fabricius ◽  
Chain Reactions ◽  
Renal Epithelium ◽  
Polymerase Chain Reactions ◽  
Transmission Electron ◽  
Intracytoplasmic Inclusion Bodies ◽  
Polymerase Chain ◽  
Streptopelia Orientalis

During the spring, an outbreak of sudden death involving 58 birds occurred in a zoo. Histopathological examinations revealed variable numbers of intracytoplasmic basophilic microorganisms in the macrophages, hepatocytes, and renal epithelium of most birds, along with occasional botryoid intracytoplasmic inclusion bodies within histiocytes in the bursa of Fabricius. Based on the results of histopathological examinations, immunohistochemical staining, transmission electron microscopy, and polymerase chain reactions, genotype B Chlamydia psittaci infection concurrent with pigeon circovirus (PiCV) was diagnosed. A retrospective survey, including two years before the outbreak and the outbreak year, of C. psittaci and PiCV infections of dead birds in the aviaries, revealed that the outbreak was an independent episode. The findings of this study indicate that concurrent infection with C. psittaci and PiCV might lead to lethal outbreaks of chlamydiosis, particularly Streptopelia orientalis. In addition, persistently monitoring both pathogens and identifying potential PiCV carriers or transmitters might also help prevent lethal disease outbreaks.

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