Respiratory contamination of polymerase chain reactions by human herpesvirus 6

1994 ◽  
Vol 47 (4) ◽  
pp. 325-327 ◽  
Author(s):  
Patrick K. Kelley ◽  
Kenneth L. McClain
Keyword(s):  
Human Herpesvirus ◽  
Human Herpesvirus 6 ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Polymerase Chain ◽  
Herpesvirus 6

Detection of human herpesvirus 6 sequences in lymphoma tissues by immunohistochemistry and polymerase chain reactions

1996 ◽  
Vol 106 (2) ◽  
pp. 221-226 ◽  
Author(s):  
Abdur Razzaque ◽  
Yvan Francillon ◽  
Pongrac N. Jilly ◽  
Frederick Varricchio
Keyword(s):  
Human Herpesvirus ◽  
Human Herpesvirus 6 ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Polymerase Chain ◽  
Herpesvirus 6

scholarly journals Human Herpesvirus 6 Meningitis in a Neonatal Case

2021 ◽  
Vol 28 (4) ◽  
pp. 153-156
Author(s):  
Gyu Min Yeon ◽  
Yu Jin Jung
Keyword(s):  
Intensive Care Unit ◽  
Neonatal Intensive Care ◽  
Neonatal Period ◽  
Newborn Infants ◽  
Human Herpesvirus ◽  
Human Herpesvirus 6 ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Herpesvirus 6 ◽  
Infancy And Early Childhood

Incidence of human herpesvirus-6 (HHV-6) infection in the neonatal period has been reported in few cases. HHV-6, commonly responsible for roseola, is known to establish infection during infancy and early childhood. A 14-day-old neonate, presented with a fever of 38.3℃, primarily due to an HHV-6 infection, was admitted to our neonatal intensive care unit. A polymerase chain reaction (PCR) of his cerebrospinal fluid was positive for HHV-6. Additionally, serology for HHV-6 PCR was positive. We believe that HHV-6 can cause infection in febrile newborn infants.

Detection of human herpesvirus-6 in paraffin-embedded tissue of cervical cancer by polymerase chain reaction

1994 ◽  
Vol 47 (3) ◽  
pp. 297-305 ◽  
Author(s):  
Hong Wang ◽  
Michael Chen ◽  
Zwi N. Berneman ◽  
Gregorio Delgado ◽  
Joseph A. DiPaolo
Keyword(s):  
Cervical Cancer ◽  
Polymerase Chain Reaction ◽  
Human Herpesvirus ◽  
Human Herpesvirus 6 ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Herpesvirus 6

scholarly journals Human Herpesvirus 6 (HHV-6) Causes Severe Thymocyte Depletion in SCID-hu Thy/Liv Mice

1999 ◽  
Vol 189 (12) ◽  
pp. 1953-1960 ◽  
Author(s):  
Alberto Gobbi ◽  
Cheryl A. Stoddart ◽  
Mauro S. Malnati ◽  
Giuseppe Locatelli ◽  
Fabio Santoro ◽  
...  
Keyword(s):  
Animal Model ◽  
Quantitative Polymerase Chain Reaction ◽  
Human Herpesvirus ◽  
Small Animal ◽  
Immunosuppressive Agent ◽  
Human Herpesvirus 6 ◽  
Small Animal Model ◽  
Thymic Tissue ◽  
Polymerase Chain ◽  
Herpesvirus 6

Human herpesvirus 6 (HHV-6) is a potentially immunosuppressive agent that may act as a cofactor in the progression of AIDS. Here, we describe the first small animal model of HHV-6 infection. HHV-6 subgroup A, strain GS, efficiently infected the human thymic tissue implanted in SCID-hu Thy/Liv mice, leading to the destruction of the graft. Viral DNA was detected in Thy/Liv implants by quantitative polymerase chain reaction (PCR) as early as 4 d after inoculation and peaked at day 14. The productive nature of the infection was confirmed by electron microscopy and immunohistochemical staining. Atypical thymocytes with prominent nuclear inclusions were detected by histopathology. HHV-6 replication was associated with severe, progressive thymocyte depletion involving all major cellular subsets. However, intrathymic T progenitor cells (ITTPs) appeared to be more severely depleted than the other subpopulations, and a preferred tropism of HHV-6 for ITTPs was demonstrated by quantitative PCR on purified thymocyte subsets. These findings suggest that thymocyte depletion by HHV-6 may be due to infection and destruction of these immature T cell precursors. Similar results were obtained with strain PL-1, a primary isolate belonging to subgroup B. The severity of the lesions observed in this animal model underscores the possibility that HHV-6 may indeed be immunosuppressive in humans.

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