Detection of residual E. coli host cell DNA by 23S ribosomal RNA gene-targeted quantitative polymerase chain reactions

2021 ◽  
Vol 198 ◽  
pp. 114000
Author(s):  
Dehua Li ◽  
Qian Zhang ◽  
Guodi Liu ◽  
Linsong Zhang ◽  
Zhangjie Gu ◽  
...  
Keyword(s):  
Host Cell ◽  
Ribosomal Rna ◽  
Ribosomal Rna Gene ◽  
Chain Reactions ◽  
E Coli ◽  
Polymerase Chain Reactions ◽  
Polymerase Chain ◽  
23S Ribosomal Rna

scholarly journals Determination of antimicrobial resistance to extended-spectrum cephalosporin, quinolones, and vancomycin in selected human enteric pathogens from Prince Edward Island, Canada

2018 ◽  
Vol 64 (7) ◽  
pp. 473-482
Author(s):  
Babafela Awosile ◽  
Gregory German ◽  
Juan Carlos Rodriguez-Lecompte ◽  
Matthew E. Saab ◽  
Luke C. Heider ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Prince Edward Island ◽  
Carbapenem Resistance ◽  
Chain Reactions ◽  
E Coli ◽  
Polymerase Chain Reactions ◽  
Extended Spectrum ◽  
Enterococcus Spp ◽  
Polymerase Chain

The aim of this study was to determine the frequency of fecal carriage of vancomycin-resistant Enterococcus spp. and Escherichia coli with reduced susceptibilities to extended-spectrum cephalosporins (ESCs) and quinolones in humans on Prince Edward Island, Canada. Convenience fecal samples from individuals on Prince Edward Island were screened phenotypically using selective culture and genotypically using multiplex polymerase chain reactions to detect E. coli and Enterococcus spp. resistant to critically important antimicrobials. Twenty-six (5.3%) of 489 individuals had E. coli with reduced susceptibility to ESCs. Twenty-five (96.2%) of the 26 isolates harbored blaTEM, 18 (69.2%) harbored blaCMY-2, 16 (61.5%) harbored blaCTX-M groups, 2 (7.7%) harbored blaSHV genes. None of the ESC-resistant E. coli was positive for carbapenem resistance. Twenty-one (8.3%) of 253 individuals had E. coli isolates with reduced quinolone susceptibility. All 21 isolates were positive for at least 1 qnr gene, with 3 (14.3%) isolates positive for qnrB, 5 (23.8%) positive for qnrS, and 13 (61.9%) positive for both qnrB and qnrS genes. All the enterococci isolates were vancomycin-susceptible. Higher susceptibility to the critically important antimicrobials was found in this study. This study can serve as a baseline for future antimicrobial resistance surveillance within this region.

scholarly journals Investigation of Lethal Concurrent Outbreak of Chlamydiosis and Pigeon Circovirus in a Zoo

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1654
Author(s):  
Wei-Tao Chen ◽  
Chin-Ann Teng ◽  
Cheng-Hsin Shih ◽  
Wei-Hsiang Huang ◽  
Yi-Fan Jiang ◽  
...  
Keyword(s):  
Disease Outbreaks ◽  
Chlamydia Psittaci ◽  
Bursa Of Fabricius ◽  
Chain Reactions ◽  
Renal Epithelium ◽  
Polymerase Chain Reactions ◽  
Transmission Electron ◽  
Intracytoplasmic Inclusion Bodies ◽  
Polymerase Chain ◽  
Streptopelia Orientalis

During the spring, an outbreak of sudden death involving 58 birds occurred in a zoo. Histopathological examinations revealed variable numbers of intracytoplasmic basophilic microorganisms in the macrophages, hepatocytes, and renal epithelium of most birds, along with occasional botryoid intracytoplasmic inclusion bodies within histiocytes in the bursa of Fabricius. Based on the results of histopathological examinations, immunohistochemical staining, transmission electron microscopy, and polymerase chain reactions, genotype B Chlamydia psittaci infection concurrent with pigeon circovirus (PiCV) was diagnosed. A retrospective survey, including two years before the outbreak and the outbreak year, of C. psittaci and PiCV infections of dead birds in the aviaries, revealed that the outbreak was an independent episode. The findings of this study indicate that concurrent infection with C. psittaci and PiCV might lead to lethal outbreaks of chlamydiosis, particularly Streptopelia orientalis. In addition, persistently monitoring both pathogens and identifying potential PiCV carriers or transmitters might also help prevent lethal disease outbreaks.

scholarly journals Role of precursor sequences in the ordered maturation of E. coli 23S ribosomal RNA

RNA ◽  
2011 ◽  
Vol 18 (2) ◽  
pp. 345-353 ◽  
Author(s):  
N. S. Gutgsell ◽  
C. Jain
Keyword(s):  
Ribosomal Rna ◽  
E Coli ◽  
23S Ribosomal Rna
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