scholarly journals 861 PB 540 In Vitro Adventitious Shoot Formation and Plant Regeneration from Culantro (Eryngium foetidium L.) Leaf

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 557a-557
Author(s):  
Yasseen Mohamed-Yasseen
Keyword(s):  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Perennial Herb ◽  
Shoot Induction ◽  
Adventitious Shoot Formation ◽  
Leaf Petiole ◽  
Shoot Number ◽  
Tropical America

Culantro is a perennial herb with odor like that of corinder, native to tropical America and the West Indies. Explants were excised from leaf petiole of mature culantro plant. Explants were cultured on MS alone or supplemented with 4.4, or 13.3 uM BA with 0.5 uM NAA, or supplemented with 0.3, 1.8, 4.5, or 3 uM Thidiazuron (TDZ) wth 0.5 uM NAA. leaf explants formed callus and were transferred to the same medium for shoot induction. Only explants which were cultured on MS supplemented with 13.3 uM BA or 0.9, l.8, 4.5, or 3 UM TDZ produced shoots. Shoots were regenerated in all TDZ-containing media with high 100% frequency. Shoot number increased with the increase of TDZ concentration but shoot length decreased. Although cytokinins are reported to inhibit root initiation, regenerated shoots formed roots with 100% frequency in BA-and TDZ-containing media. Regenerated shoots were transferred to MS containing 3.9 UM TDZ for further growth. Rooted shoots were transferred to soil and normal plants were obtained.

scholarly journals Regeneration of Pelargonium in vitro

2013 ◽  
Vol 56 (1-2) ◽  
pp. 177-190
Author(s):  
Agnieszka Wojtania ◽  
Wiesław Szafrański
Keyword(s):  
Somatic Embryos ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Medium Composition ◽  
High Tendency ◽  
Adventitious Shoot Formation ◽  
In Vitro Techniques ◽  
Tissue Culture Propagation

<i>Pelargonium</i> sp. has been a subject of numerous studies to deterimine the effec tiveness of in vitro techniques to produce a large number of pathogen-free plants. Regeneration of pelargonium plants from the different initial explants as well via organogenesis as via somatic embryogenesis has been obtained. The most effective adventitious shoot formation has been achieved from shoot tips and axillary buds using cytokinin or cytokinin/auxin combinations. Leaf explants, whose general have lower organogenic potency, regenerate better in the presence of thidiazuron. This growth regulator stimulate the somatic embryos production from hypocotyl and cotyledone explants too. The main problem in tissue culture propagation of <i>Pelargonium</i> has been the high tendency to formation of vigorously growing callus with low organogenic potency and rapid senescence of cultures. Moreover, the significant differen ces in requirements to the medium composition (minerals, organic compounds and growth regulators) between <i>Pelargonium</i> cultivars has been observed. This makes difficult to develop an universaI method of <i>Pelargonium</i> micropropagation.

Adventitious shoot formation from in vitro leaf explants of carnation (Dianthus caryophyllus L.)

1992 ◽  
Vol 51 (3-4) ◽  
pp. 223-235 ◽  
Author(s):  
A.C. van Altvorst ◽  
H.J.J. Koehorst ◽  
T. Bruinsma ◽  
J. Jansen ◽  
J.B.M. Custers ◽  
...  
Keyword(s):  
Dianthus Caryophyllus ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Adventitious Shoot Formation

scholarly journals Effects of Colchicine and Oryzalin on Callus and Adventitious Shoot Formation of Euphorbia pulchurrima `Winter Rose'

HortScience ◽  
2006 ◽  
Vol 41 (7) ◽  
pp. 1651-1655 ◽  
Author(s):  
Kimberly A. Pickens ◽  
(Max) Z.-M. Cheng ◽  
Stephen A. Kania
Keyword(s):  
Flow Cytometry ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Shoot Induction ◽  
Adventitious Shoot Formation ◽  
Leaf Tissues ◽  
Mitotic Inhibitors ◽  
In Vitro Induction

The mitotic inhibitors, colchicine and oryzalin, were evaluated for their effects on callus, adventitious shoot formation, and tetraploid induction of Euphorbia pulchurrima `Winter Rose'. In vitro grown leaf sections were placed on various media supplemented with either colchicine or oryzalin at various concentrations for 1 to 4 days. Colchicine was less damaging to leaf tissues than oryzalin. On various colchicine-containing media, prolific calluses were produced and adventitious shoot formation was observed. Regenerated shoots were found to be diploid as determined by flow cytometry. On media supplemented with oryzalin (28.9 μm to 144 μm), leaf tissues produced callus but failed to form adventitious shoots. Samples of calluses produced on oryzalin-containing media were subject to analysis using flow cytometry and were found to be diploid. These results suggest that the colchicine is less toxic on poinsettia tissues and shoot induction than oryzalin. Additional experiments are needed to establish a protocol for in vitro induction of poinsettia tetraploid with colchicine and oryzalin.

scholarly journals Regenerating Plants from in Vitro Culture of Black Locust Cotyledon and Leaf Explants

HortScience ◽  
1993 ◽  
Vol 28 (9) ◽  
pp. 942-945 ◽  
Author(s):  
I. Arrillaga ◽  
S.A. Merkle
Keyword(s):  
Shoot Regeneration ◽  
Black Locust ◽  
Robinia Pseudoacacia ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Regeneration Frequency ◽  
Adventitious Shoot Formation

A protocol to achieve efficient plant regeneration from juvenile black locust (Robinia pseudoacacia L.) explants is described. Direct adventitious shoots were induced from cotyledon explants on woody plant medium containing 22.2 μm BA and 0.4 μm 2,4-D. Shoots developed and new shoots were induced when the explants were transferred to medium without growth regulators. The effect of dark incubation on shoot regeneration from cotyledons indicated that 15 days of darkness resulted in a high regeneration frequency (91.7%). Adventitious shoot formation also was induced from sections of in vitro-derived leaves cultured in darkness on Murashige and Skoog medium supplemented with 4.4 μm BA and 24.6 μm IBA. A shoot regeneration frequency of 89% was obtained when explants were subcultured on a medium containing 4.4 μm BA and 0.5 μm IAA. Shoots were rooted on Schenk and Hildebrandt medium with or without IBA. Plantlets were acclimatized and grown in the greenhouse. Chemical names used: N -(phenylmethyl)-1H -purin-6-amine (BA); 2,4-dichlorophenoxyacetic acid (2,4-D); indole-3-acetic acid (IAA); indole-3-butyric acid (IBA).

scholarly journals In vitro Regeneration of the Medicinal Plant, Plumbago zeylanica L. with Reference to a Unique Population in Maruthamalai, the Western Ghats, India

1970 ◽  
Vol 18 (2) ◽  
pp. 173-179 ◽  
Author(s):  
T. Mallikadevi ◽  
P. Senthilkumar ◽  
S. Paulsamy
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Garden Soil ◽  
Plumbago Zeylanica ◽  
Adventitious Shoot Formation ◽  
The Western Ghats

The in vitro regeneration of Plubago zeylanica exhibited that the callus was initiated in the basal medium containing BAP, NAA, 2, 4-D, and IBA.  The high amount (90%) of organic calli was induced in the basal medium supplemented with 2, 4-D, alone at 2.0 mg/l. In the subculture the adventitious shoot formation was prominently higher (83%) in the basal medium containing BAP, and NAA at 3.5 and 0.3 mg/l, respectively. IAA (1.0 mg/l)effectively produced higher percen-tage (90) of roots and root growth. After sequential hardening, survivability rate was observed to be significantly higher (80%) in the hardening medium containing garden soil, sand and vermicompost in the ratio of 1 : 1 : 1 by volume under greenhouse condition.  Key words: Plumbago zeylanica, In vitro regeneration, Medicinal plant D.O.I. 10.3329/ptcb.v18i2.3648 Plant Tissue Cult. & Biotech. 18(2): 173-179, 2008 (December)

Adventitious shoot formation from leaf explants of Rhododendron

1992 ◽  
Vol 30 (3) ◽  
pp. 237-241 ◽  
Author(s):  
Giovanni Iapichino ◽  
Steve MeCullech ◽  
Tony H. H. Chen
Keyword(s):  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Adventitious Shoot Formation
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