Adventitious shoot formation from in vitro leaf explants of carnation (Dianthus caryophyllus L.)

1992 ◽  
Vol 51 (3-4) ◽  
pp. 223-235 ◽  
Author(s):  
A.C. van Altvorst ◽  
H.J.J. Koehorst ◽  
T. Bruinsma ◽  
J. Jansen ◽  
J.B.M. Custers ◽  
...  
Keyword(s):  
Dianthus Caryophyllus ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Adventitious Shoot Formation

scholarly journals Regeneration of Pelargonium in vitro

2013 ◽  
Vol 56 (1-2) ◽  
pp. 177-190
Author(s):  
Agnieszka Wojtania ◽  
Wiesław Szafrański
Keyword(s):  
Somatic Embryos ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Medium Composition ◽  
High Tendency ◽  
Adventitious Shoot Formation ◽  
In Vitro Techniques ◽  
Tissue Culture Propagation

<i>Pelargonium</i> sp. has been a subject of numerous studies to deterimine the effec tiveness of in vitro techniques to produce a large number of pathogen-free plants. Regeneration of pelargonium plants from the different initial explants as well via organogenesis as via somatic embryogenesis has been obtained. The most effective adventitious shoot formation has been achieved from shoot tips and axillary buds using cytokinin or cytokinin/auxin combinations. Leaf explants, whose general have lower organogenic potency, regenerate better in the presence of thidiazuron. This growth regulator stimulate the somatic embryos production from hypocotyl and cotyledone explants too. The main problem in tissue culture propagation of <i>Pelargonium</i> has been the high tendency to formation of vigorously growing callus with low organogenic potency and rapid senescence of cultures. Moreover, the significant differen ces in requirements to the medium composition (minerals, organic compounds and growth regulators) between <i>Pelargonium</i> cultivars has been observed. This makes difficult to develop an universaI method of <i>Pelargonium</i> micropropagation.

scholarly journals 861 PB 540 In Vitro Adventitious Shoot Formation and Plant Regeneration from Culantro (Eryngium foetidium L.) Leaf

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 557a-557
Author(s):  
Yasseen Mohamed-Yasseen
Keyword(s):  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Perennial Herb ◽  
Shoot Induction ◽  
Adventitious Shoot Formation ◽  
Leaf Petiole ◽  
Shoot Number ◽  
Tropical America

Culantro is a perennial herb with odor like that of corinder, native to tropical America and the West Indies. Explants were excised from leaf petiole of mature culantro plant. Explants were cultured on MS alone or supplemented with 4.4, or 13.3 uM BA with 0.5 uM NAA, or supplemented with 0.3, 1.8, 4.5, or 3 uM Thidiazuron (TDZ) wth 0.5 uM NAA. leaf explants formed callus and were transferred to the same medium for shoot induction. Only explants which were cultured on MS supplemented with 13.3 uM BA or 0.9, l.8, 4.5, or 3 UM TDZ produced shoots. Shoots were regenerated in all TDZ-containing media with high 100% frequency. Shoot number increased with the increase of TDZ concentration but shoot length decreased. Although cytokinins are reported to inhibit root initiation, regenerated shoots formed roots with 100% frequency in BA-and TDZ-containing media. Regenerated shoots were transferred to MS containing 3.9 UM TDZ for further growth. Rooted shoots were transferred to soil and normal plants were obtained.

scholarly journals Regenerating Plants from in Vitro Culture of Black Locust Cotyledon and Leaf Explants

HortScience ◽  
1993 ◽  
Vol 28 (9) ◽  
pp. 942-945 ◽  
Author(s):  
I. Arrillaga ◽  
S.A. Merkle
Keyword(s):  
Shoot Regeneration ◽  
Black Locust ◽  
Robinia Pseudoacacia ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Regeneration Frequency ◽  
Adventitious Shoot Formation

A protocol to achieve efficient plant regeneration from juvenile black locust (Robinia pseudoacacia L.) explants is described. Direct adventitious shoots were induced from cotyledon explants on woody plant medium containing 22.2 μm BA and 0.4 μm 2,4-D. Shoots developed and new shoots were induced when the explants were transferred to medium without growth regulators. The effect of dark incubation on shoot regeneration from cotyledons indicated that 15 days of darkness resulted in a high regeneration frequency (91.7%). Adventitious shoot formation also was induced from sections of in vitro-derived leaves cultured in darkness on Murashige and Skoog medium supplemented with 4.4 μm BA and 24.6 μm IBA. A shoot regeneration frequency of 89% was obtained when explants were subcultured on a medium containing 4.4 μm BA and 0.5 μm IAA. Shoots were rooted on Schenk and Hildebrandt medium with or without IBA. Plantlets were acclimatized and grown in the greenhouse. Chemical names used: N -(phenylmethyl)-1H -purin-6-amine (BA); 2,4-dichlorophenoxyacetic acid (2,4-D); indole-3-acetic acid (IAA); indole-3-butyric acid (IBA).

scholarly journals In vitro Regeneration of the Medicinal Plant, Plumbago zeylanica L. with Reference to a Unique Population in Maruthamalai, the Western Ghats, India

1970 ◽  
Vol 18 (2) ◽  
pp. 173-179 ◽  
Author(s):  
T. Mallikadevi ◽  
P. Senthilkumar ◽  
S. Paulsamy
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Garden Soil ◽  
Plumbago Zeylanica ◽  
Adventitious Shoot Formation ◽  
The Western Ghats

The in vitro regeneration of Plubago zeylanica exhibited that the callus was initiated in the basal medium containing BAP, NAA, 2, 4-D, and IBA.  The high amount (90%) of organic calli was induced in the basal medium supplemented with 2, 4-D, alone at 2.0 mg/l. In the subculture the adventitious shoot formation was prominently higher (83%) in the basal medium containing BAP, and NAA at 3.5 and 0.3 mg/l, respectively. IAA (1.0 mg/l)effectively produced higher percen-tage (90) of roots and root growth. After sequential hardening, survivability rate was observed to be significantly higher (80%) in the hardening medium containing garden soil, sand and vermicompost in the ratio of 1 : 1 : 1 by volume under greenhouse condition.  Key words: Plumbago zeylanica, In vitro regeneration, Medicinal plant D.O.I. 10.3329/ptcb.v18i2.3648 Plant Tissue Cult. & Biotech. 18(2): 173-179, 2008 (December)

Adventitious shoot formation from leaf explants of Rhododendron

1992 ◽  
Vol 30 (3) ◽  
pp. 237-241 ◽  
Author(s):  
Giovanni Iapichino ◽  
Steve MeCullech ◽  
Tony H. H. Chen
Keyword(s):  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Adventitious Shoot Formation

In vitro studies of adventitious shoot formation in Pinus contorta

1981 ◽  
Vol 59 (5) ◽  
pp. 870-874 ◽  
Author(s):  
Sara Von Arnold ◽  
Tage Eriksson
Keyword(s):  
Pinus Contorta ◽  
Stem Elongation ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Adventitious Buds ◽  
Adventitious Shoot Formation ◽  
Bud Induction ◽  
Further Development

Isolated embryos of Pinus contorta Dougl. ex Loud, were induced to form adventitious buds on a cytokinin-supplemented medium. Further development of the buds required transfer to a cytokininless medium. Both bud induction and development were stimulated by a dilution of the basal culture medium and best growth was obtained if the buds were isolated from the original tissue when stem elongation had started. The growth of these isolated adventitious shoots was further stimulated by adding activated charcoal to the diluted medium. A small percentage of the shoots have been rooted. The capacity for bud formation varied among seeds collected from different regions of British Columbia. This method for induction of adventitious buds on embryos was also applicable to explants of young seedlings.

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