scholarly journals 331 Effects of Explant Type, Sucrose Level and Callus Development Time on In Vitro Plant Regeneration of Sweetpotato

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 449A-449
Author(s):  
L. Chen ◽  
S.O. Park ◽  
S. Dhir ◽  
A.S. Bhagsari
Keyword(s):  
Plant Regeneration ◽  
Plant Growth ◽  
Growth Regulators ◽  
Somatic Embryos ◽  
Development Time ◽  
Shoot Tip ◽  
Explant Type ◽  
Callus Initiation ◽  
Sucrose Level

Several experiments were conducted to evaluate the influence of explant type, sucrose level, and callus development time on sweetpotato [Ipomoea batatas (L.) Lam] in vitro culture. Shoot tip, petiole, and leaf of Selection 75-96-1 was used as explants in Murashige and Skoog (MS) media with different plant growth regulators. Calli derived from shoot tip and petiole produced 42.1% and 10.3% somatic embryos, respectively, but the leaf failed to produce somatic embryos. The effect of sucrose level was determined using shoot tip as explants. Compared with 3% sucrose in the same plant growth regulators level medium during callus initiation and callus proliferation periods, 5% sucrose level suppressed root growth and improved shoot regeneration. The callus development time was measured by using shoot tips on callus initiation medium containing 1.5 mg/L alpha-Naphthaleneacetic acid (NAA) and 0.25 mg/L Kinetin (KIN) plus 5% sucrose. When explants were cultured for less than 6 weeks during callus initiation, then transferred onto plant regeneration medium, plant regeneration via organogenesis occurred; whereas, maintaining cultures for more than 12 weeks on the same callus initiation/proliferation medium, plant regeneration was favored via embryogenesis. Explant type and other factors affecting plant regeneration noted here could be applied to protoplast culture, somatic hybridization, and transformation in sweetpotato.

scholarly journals Influence of growth regulators on plant regeneration in tomato

2011 ◽  
Vol 32 (No. 3) ◽  
pp. 118-122
Author(s):  
J. Gubiš ◽  
Z. Lajchová ◽  
L. Klčová ◽  
Z. Jureková
Keyword(s):  
Plant Regeneration ◽  
Lycopersicon Esculentum ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Regeneration ◽  
Ms Medium ◽  
Explant Type ◽  
Regeneration Capacity

We studied the effect of different plant growth regulators on in vitro regeneration and plant growth of three cultivars of tomato (Lycopersicon esculentum Mill.) from explants derived from hypocotyls and cotyledons of aseptically grown seedlings. The regeneration capacity was significantly influenced by cultivar and explant type. The highest number of shoots regenerated in both types of explants was recorded on MS medium supplemented with 1.0 mg/dm<sup>3</sup> zeatin and 0.1 mg/dm<sup>3</sup> IAA. The cultivar UC 82 showed the best regeneration capacity on all types of used media. The most responsive explants were hypocotyls with 90&ndash;92% regeneration in dependence on the used cultivars and mean production from 0.18 to 0.38 shoots per explant. &nbsp;

scholarly journals In vitro organogenesis and plant regeneration of Passiflora xishuangbannaensis, a species with extremely small populations

2021 ◽  
Author(s):  
Yuan-yuan Meng ◽  
Shi-jie Song ◽  
Sven Landrein
Keyword(s):  
Plant Regeneration ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Basal Medium ◽  
Ex Situ ◽  
Naphthaleneacetic Acid ◽  
South American ◽  
South American Species

Abstract Passiflora xishuangbannaensis (Passifloraceae) is endemic to a few sites of Mengyang nature reserve in Yunnan, Xishuangbanna and less than 40 individuals have been recorded. Nine Passiflora species are endemic to Yunnan with most species occurring in South America, making P. xishuangbannaensis highly significant and emblematic to the conservation work in the region. This study is designed to provide the first protocol for in vitro organogenesis and plant regeneration for ex situ conservation and reintroduction for an Asian Passiflora species. Using internodes, petioles and tendrils we optimize calli formation and root elongation using several plant growth regulators, individually or in combination. We also assess the genetic stability of regenerated cells. The maximum callus induction and shoot bud differentiation were both achieved on half Murashige and Skoog basal medium supplemented with 4.44 µM 6-Benzylaminopurine and 1.08 µM 1-Naphthaleneacetic acid. The best rooting was achieved from 30 days old, regenerated shoots on half Murashige and Skoog basal medium supplemented with 1.08 µM 1-Naphthaleneacetic acid. Micropropagated plants were subjected to inter simple sequence repeat markers analyses. Collectively, 86 bands were generated from 6 primers of which 12 bands were polymorphic, showing genetic variation between the regenerated plantlets and the original plant. Response to plant growth regulators was more specific than most other studies using South American species, which could be explained by the morphological and physiological differences between South American and Asian Passiflora species

scholarly journals EFFECT OF THE MAIN CYTOKININS ON ANDROGENESIS OF WHITE CABBAGE (BRASSICA OLERACEA L. var. CAPITATA) ANTHERS CULTIVATED “IN VITRO”

2020 ◽  
Vol 1 (1) ◽  
pp. 9-14
Author(s):  
Tina Oana Cristea ◽  
Alin Gabriel Iosob ◽  
Creola Brezeanu ◽  
Petre Marian Brezeanu ◽  
Dan Avasiloaiei ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Plant Growth ◽  
Growth Regulators ◽  
In Vitro Regeneration ◽  
Research Work ◽  
White Cabbage ◽  
Brassica Oleracea L ◽  
Main Growth

The aim of the present research work was the screening of the effect of the main cytokinin (BAP, kinetin or zeatin) in different concentrations and combinations with the auxin NAA on androgenesis of white cabbage anthers cultivated in vitro. The results obtained are regarded as an intermediary stage for the development of a reproducible protocol for in vitro regeneration of plant from anther culture. Thus, for the determination of the influence of plant growth regulators formula over the callus induction and plant regeneration from anthers cultivated in vitro in the present study the authors undergo a screening of the three most frequently utilized cytokinins (BAP, kinetin and zeatin) in different concentration and combination with the auxin NAA. The results obtained, indicated that the best morphogenetic reaction is obtained on variant with BAP as the main growth regulator.

scholarly journals The Influence of Plant Growth Regulators on Budbreak and Shoot Growth from Large Stem Segments of Acer saccharinum L.

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 984C-984
Author(s):  
Katayoun Mansouri ◽  
John E. Preece
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Tip ◽  
Old Field ◽  
Latex Paint ◽  
Acer Saccharinum ◽  
Shoot Tip Explants ◽  
Stem Segments

A factorial combination of gibberellic acid (GA3) and benzyladenine (BA) was applied in 20% white exterior latex paint to large (40 cm long, >2.5 cm diameter) stem segments of Acer saccharinum L. (silver maple) to determine the effects on forcing new softwood shoots in the greenhouse or laboratory and the subsequent growth of these new shoots in vitro. Stem segments were harvested from 10-year-old field-grown coppice shoots. The GA3/BA-paint mixes were applied to the entire stem segments that were forced in plastic flats filled with 1 perlite: 1 vermiculite (by volume) and watered with care so as not to wet the new softwood shoots. The flats and stem segments were drenched weekly with Zerotol (0.18% H2O2). The softwood shoots were harvested when they were at least 3 cm long. After disinfesting and rinsing, the nodal and shoot tip explants were established aseptically in vitro on DKW medium with no cytokinin or with 10-8M thidiazuron. Coppice shoots were harvested, cut, and painted on 9 Sept., 28 Oct., and 12 Dec. 2005. Although there were no significant differences in shoot production among stem segments painted with various combinations of GA3/BA, stems treated with plant growth regulators produced a mean of 2.7, 1.8, or 0.5 shoots for the three harvest dates compared to 0.5, 0.0, or 0.25 shoots on control stem segments. It is well-known that shoot forcing is poor from September through January; however, use of GA3/BA resulted in growth of dormant epicormic shoots. Shoot tip explants produced the most shoots in vitro after 8 weeks if they were harvested from stem segments treated with 0.03 mM GA3, whereas nodal explants produced the most shoots if harvested from segments that had been treated with 0.01 mM GA3.

scholarly journals In vitro Plant Regeneration of Magnolia punduana: An Endemic and Threatened Plant Species

2017 ◽  
Vol 27 (2) ◽  
pp. 153-159 ◽  
Author(s):  
Rajib Borah ◽  
Suman Kumaria ◽  
Hiranjit Choudhury
Keyword(s):  
Plant Regeneration ◽  
Plant Growth ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
In Vitro Plant Regeneration ◽  
Threatened Plant Species ◽  
Callus Initiation ◽  
Half Strength ◽  
Basal Media

Shoots were induced from axillary and nodal buds of Magnolia punduna on MS supplemented with 0.1 μm of BAP. Out of five basal media tested (MS, ½ MS, ¼ MS, LS and WP), MS was found to be most effective for shoot and callus initiation. Different plant growth regulators (0.1 ‐ 1.0 μm) induced shoot formation in different proportions. The combination of 0.1 μm IBA and 0.5 μm BAP was found optimum for shoot elongation with minimal necrosis of the explants. Half strength of MS supplemented with 8.0 μm IBA was found suitable for rooting.Plant Tissue Cult. & Biotech. 27(2): 153-159, 2017 (December)

scholarly journals EFFECT OF EXPALNT TYPE AND SOME PLANT GROWTH REGULATORS ON CULTURE INITIATION OF STEVIA PLANTS IN VITRO

2017 ◽  
Vol 48 (5) ◽  
Author(s):  
Khierallah & Al-Obaidy
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Tips ◽  
Control Treatment ◽  
Ms Medium ◽  
Explant Type ◽  
Culture Initiation ◽  
The Impact

This research was conducted in order to study the effect of explant type and some plant growth regulators on culture initiation of Stevia rebaudiana Bertoni in vitro. The experiments included surface sterilization and test two types of explants (shoot tips and stem nodes) and the impact of KIN and BA and IAA and IBA in the cultures initiation. Results revealed the efficiency of sodium hypochlorite (NaOCl) for disinfestation of explant at 0.050% concentration giving less contamination for shoot tips and stem nods (10% and 20% respectively). Results showed that shoot tips inoculated in MS medium plus KIN at 0.3 mg. L-1 was significantly increase the number of regenerated shoots as it produced 4.2 shoots per explant while medium without cytokinin (control) produced less number of shoots reached 1.4 shoots per explant. KIN treatment reduced shoots length as control treatment produced the highest length (6.74 cm).  The interaction between the explant type and BA concentration was significantly increase the number of regenerated shoots as shoot tips produced 3.6 shoots per explant in MS medium supplemented with 0.1 mg. L-1. BA treatment reduced shoots length as control treatment produced the highest length (6.74 cm). No positive effect was gain when auxins (IBA and IAA) were added in combination with cytokinin in culture medium. The above results can be adopted to established stevia in vitro culture successfully.

scholarly journals In vitro plant regeneration of Passiflora setacea D.C. (Passifloraceae): the influence of explant type, growth regulators, and incubation conditions

2014 ◽  
Vol 50 (6) ◽  
pp. 738-745 ◽  
Author(s):  
Lorena Melo Vieira ◽  
Diego Ismael Rocha ◽  
Mariana Futia Taquetti ◽  
Luzimar Campos da Silva ◽  
José Marcello Salabert de Campos ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Growth Regulators ◽  
Explant Type ◽  
In Vitro Plant Regeneration ◽  
In Vitro Plant
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