GENETIC LINKAGE MAP FOR WATERMELON: SEGREGATION AND DISTRIBUTION OF DNA MARKERS

Genetic linkage map is being constructed for watermelon based on a testcross population and an F2 population. About 51.0% and 31.8% of the markers in the testcross and F2 populations are skewed form the expected segregation ratios. AFLP markers appeared to be clustered on linkage regions, while ISSR and RAPD markers are randomly dispersed on the genome. AFLP markers also have greater genetic distances as compared with ISSR and RAPD markers, resulting in significant increase of map distance. An initial genetic map (based on the testcross population) that contains 27 ISSR and 141 RAPD markers has a total linkage distance of 1,166.2 cM. The addition of 2 ISSR, 8 RAPD and 77 AFLP markers increased the genetic distance of the map to 2,509.9 cM. Similar results with AFLP markers were also shown in mapping experiments with an F2S7 recombinant inbred line (RIL) population that was recently constructed for watermelon. Although the skewed segregation, marker order appeared to be consistent in linkage groups of the testcross and the F2 population. Experiments with SSR, and EST markers are being conducted to saturate the linkage map of watermelon genome.

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A genetic linkage map for Stevia rebaudiana

Genome ◽

10.1139/g98-161 ◽

1999 ◽

Vol 42 (4) ◽

pp. 657-661 ◽

Cited By ~ 7

Author(s):

Y Yao ◽

M Ban ◽

J Brandle

Keyword(s):

Linkage Map ◽

Genetic Linkage ◽

Rapd Markers ◽

Genetic Linkage Map ◽

Average Distance ◽

Stevia Rebaudiana ◽

Linkage Groups ◽

Genome Map ◽

High Level ◽

Map Distance

To lay a foundation for molecular breeding efforts, the first genetic linkage map for Stevia rebaudiana has been constructed using segregation data from a pseudo test-cross F1 population. A total of 183 randomly amplified polymorphic DNA (RAPD) markers were analysed and assembled into 21 linkage groups covering a total distance of 1389 cM, with an average distance between markers of of 7.6 cM. The 11 largest linkage groups consisted of 4-19 loci, ranged in length from 56 to 174 cM, and accounted for 75% of the total map distance. Fifteen RAPD loci were found to be unlinked. From the 521 primers showing amplification products, 185 (35.5%) produced a total of 293 polymorphic fragments, indicating a high level of genetic diversity in stevia. Most of the RAPD markers in stevia segregated in normal Mendelian fashion.Key words: stevia, open-pollinated, genome map, RAPD.

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A genetic linkage map of crested wheatgrass based on AFLP and RAPD markers

Genome ◽

10.1139/g2012-014 ◽

2012 ◽

Vol 55 (4) ◽

pp. 327-335 ◽

Cited By ~ 10

Author(s):

Xiaoxia Yu ◽

Xiaolei Li ◽

Yanhong Ma ◽

Zhuo Yu ◽

Zaozhe Li

Keyword(s):

Molecular Markers ◽

Linkage Map ◽

Genetic Linkage ◽

Rapd Markers ◽

Genetic Linkage Map ◽

Mapping Population ◽

Gene Localization ◽

Linkage Groups ◽

Crested Wheatgrass ◽

Map Distance

Using a population of 105 interspecific F2 hybrids derived from a cross between Agropyron mongolicum Keng and Agropyron cristatum (L.) Gaertn. ‘Fairway’ as a mapping population, a genetic linkage map of crested wheatgrass was constructed based on AFLP and RAPD molecular markers. A total of 175 markers, including 152 AFLP and 23 RAPD markers, were ordered in seven linkage groups. The map distance was 416 cM, with a mean distance of 2.47 cM between markers. The number of markers ranged from 13 to 46 in each linkage group and the length of groups ranged from 18 to 104 cM. The research found that 30 out of 175 molecular markers showed segregation distortion, accounting for 17% of all markers. This is the first genetic linkage map of crested wheatgrass. This map will facilitate gene localization, cloning, and molecular marker-assisted selection in the future.

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DEVELOPING GENETIC LINKAGE MAP AND CDNA SUBTRACTION LIBRARY FOR WATERMELON

HortScience ◽

10.21273/hortsci.40.3.871e ◽

2005 ◽

Vol 40 (3) ◽

pp. 871e-872

Author(s):

A. Levi ◽

C. E. Thomas ◽

A. Davis ◽

O.U.K. Reddy ◽

Y. Xu ◽

...

Keyword(s):

Linkage Map ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Medium Size ◽

Local Alignment ◽

Maturation Stage ◽

Cdna Clones ◽

Linkage Groups ◽

F2 Population ◽

Skewed Segregation

Genetic linkage map is being constructed for watermelon based on a testcross population and an F2 population. The testcross map comprises 262 markers (RAPD, ISSR, AFLP, SSR and ASRP markers) and covers 1,350 cM. The map comprises 11 large linkage groups (50.7–155.2 cM), 5 medium-size linkage groups (37.5–46.2 cM), and 16 small linkage groups (4.2–31.4 cM). Most AFLP markers are clustered on two linkage regions, while all other marker types are randomly dispersed on the genome. Many of the markers in this study are skewed from the classical (Mendelian) segregation ratio of1:1 in the testcross or the 3:1 ratio in the F2 population. Although the skewed segregation, marker order appeared to be consistent in linkage groups of the testcross and F2 population. A cDNA library was constructed using RNA isolated from watermelon flesh 1 week (rapid cell division stage), 2 weeks (cell growth and storage deposition stage, 4 weeks (maturation stage), and 5 weeks (postmaturation stage) post pollination. Over 1,020 cDNA clones were sequenced, and were analyzed using the Basic Local Alignment Search Tool (BLAST). The sequenced cDNA clones were designated as expressed sequenced tag (EST) markers and will be used in mapping analysis of watermelon genome.

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A genetic linkage map of Lens sp. based on microsatellite and AFLP markers and the localization of fusarium vascular wilt resistance

Theoretical and Applied Genetics ◽

10.1007/s00122-004-1892-5 ◽

2005 ◽

Vol 110 (4) ◽

pp. 669-677 ◽

Cited By ~ 115

Author(s):

A. Hamwieh ◽

S. M. Udupa ◽

W. Choumane ◽

A. Sarker ◽

F. Dreyer ◽

...

Keyword(s):

Linkage Map ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Aflp Markers ◽

Vascular Wilt ◽

Wilt Resistance

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Genetic linkage map of ISSR and RAPD markers in Einkorn wheat in relation to that of RFLP markers

Theoretical and Applied Genetics ◽

10.1007/s001220050706 ◽

1998 ◽

Vol 96 (1) ◽

pp. 37-45 ◽

Cited By ~ 76

Author(s):

T. Kojima ◽

T. Nagaoka ◽

K. Noda ◽

Y. Ogihara

Keyword(s):

Linkage Map ◽

Genetic Linkage ◽

Rapd Markers ◽

Genetic Linkage Map ◽

Rflp Markers ◽

Einkorn Wheat

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CONSTRUCTION OF A LYCHEE GENETIC LINKAGE MAP BASED ON RAPD MARKERS

Acta Horticulturae ◽

10.17660/actahortic.2003.625.13 ◽

2003 ◽

pp. 131-136 ◽

Cited By ~ 3

Author(s):

C. Liu ◽

M. Mei

Keyword(s):

Linkage Map ◽

Genetic Linkage ◽

Rapd Markers ◽

Genetic Linkage Map

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Two genetic linkage maps of mungbean using RFLP and RAPD markers

Australian Journal of Agricultural Research ◽

10.1071/ar99052 ◽

2000 ◽

Vol 51 (4) ◽

pp. 415 ◽

Cited By ~ 39

Author(s):

C. J. Lambrides ◽

R. J. Lawn ◽

I. D. Godwin ◽

J. Manners ◽

B. C. Imrie

Keyword(s):

Linkage Map ◽

Segregation Distortion ◽

Recombinant Inbred ◽

Genetic Linkage ◽

Rapd Markers ◽

Rflp Markers ◽

Linkage Maps ◽

Linkage Groups ◽

Genetic Linkage Maps ◽

Map Distance

Two genetic linkage maps of mungbean derived from the cross Berken ACC 41 are reported. The F2 map constructed from 67 individuals consisted of 110 markers (52 RFLP and 56 RAPD) that grouped into 12 linkage groups. The linked markers spanned a total map distance of 758.3 cM. A recombinant inbred (RI) population derived from the 67 F2 individuals was used for the generation of an additional linkage map. The RI map, composed entirely of RAPD markers, consisted of 115 markers in 12 linkage groups. The linked markers spanned a total map distance of 691.7 cM. Using a framework set of RFLP markers, the F2 map was compared with another F2 mungbean map constructed in Minnesota. In general, the order of these markers was consistent between maps. Segregation distortion was observed for some markers. 14.5% (16/110) of mapped F2 markers and 24% (28/115) of mapped RI markers segregated with distorted ratios. Segregation distortion occurred in each successive generation after the F2 . The regions of distortion identified in the Australian maps did not coincide with regions of the Minnesota map.

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