A genetic linkage map of crested wheatgrass based on AFLP and RAPD markers

Genome ◽  
2012 ◽  
Vol 55 (4) ◽  
pp. 327-335 ◽  
Author(s):  
Xiaoxia Yu ◽  
Xiaolei Li ◽  
Yanhong Ma ◽  
Zhuo Yu ◽  
Zaozhe Li
Keyword(s):  
Molecular Markers ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Mapping Population ◽  
Gene Localization ◽  
Linkage Groups ◽  
Crested Wheatgrass ◽  
Map Distance

Using a population of 105 interspecific F2 hybrids derived from a cross between Agropyron mongolicum Keng and Agropyron cristatum (L.) Gaertn. ‘Fairway’ as a mapping population, a genetic linkage map of crested wheatgrass was constructed based on AFLP and RAPD molecular markers. A total of 175 markers, including 152 AFLP and 23 RAPD markers, were ordered in seven linkage groups. The map distance was 416 cM, with a mean distance of 2.47 cM between markers. The number of markers ranged from 13 to 46 in each linkage group and the length of groups ranged from 18 to 104 cM. The research found that 30 out of 175 molecular markers showed segregation distortion, accounting for 17% of all markers. This is the first genetic linkage map of crested wheatgrass. This map will facilitate gene localization, cloning, and molecular marker-assisted selection in the future.

A genetic linkage map for Stevia rebaudiana

Genome ◽  
1999 ◽  
Vol 42 (4) ◽  
pp. 657-661 ◽  
Author(s):  
Y Yao ◽  
M Ban ◽  
J Brandle
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Average Distance ◽  
Stevia Rebaudiana ◽  
Linkage Groups ◽  
Genome Map ◽  
High Level ◽  
Map Distance

To lay a foundation for molecular breeding efforts, the first genetic linkage map for Stevia rebaudiana has been constructed using segregation data from a pseudo test-cross F1 population. A total of 183 randomly amplified polymorphic DNA (RAPD) markers were analysed and assembled into 21 linkage groups covering a total distance of 1389 cM, with an average distance between markers of of 7.6 cM. The 11 largest linkage groups consisted of 4-19 loci, ranged in length from 56 to 174 cM, and accounted for 75% of the total map distance. Fifteen RAPD loci were found to be unlinked. From the 521 primers showing amplification products, 185 (35.5%) produced a total of 293 polymorphic fragments, indicating a high level of genetic diversity in stevia. Most of the RAPD markers in stevia segregated in normal Mendelian fashion.Key words: stevia, open-pollinated, genome map, RAPD.

scholarly journals A Combined Amplified Fragment Length Polymorphism and Randomly Amplified Polymorphism DNA Genetic Linkage Map of Mycosphaerella graminicola, the Septoria Tritici Leaf Blotch Pathogen of Wheat

Genetics ◽  
2002 ◽  
Vol 161 (4) ◽  
pp. 1497-1505
Author(s):  
Gert H J Kema ◽  
Stephen B Goodwin ◽  
Sonia Hamza ◽  
Els C P Verstappen ◽  
Jessica R Cavaletto ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Linkage Map ◽  
Mating Type ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Mycosphaerella Graminicola ◽  
Septoria Tritici ◽  
Biological Traits ◽  
Linkage Groups

Abstract An F1 mapping population of the septoria tritici blotch pathogen of wheat, Mycosphaerella graminicola, was generated by crossing the two Dutch field isolates IPO323 and IPO94269. AFLP and RAPD marker data sets were combined to produce a high-density genetic linkage map. The final map contained 223 AFLP and 57 RAPD markers, plus the biological traits mating type and avirulence, in 23 linkage groups spanning 1216 cM. Many AFLPs and some RAPD markers were clustered. When markers were reduced to 1 per cluster, 229 unique positions were mapped, with an average distance of 5.3 cM between markers. Because M. graminicola probably has 17 or 18 chromosomes, at least 5 of the 23 linkage groups probably will need to be combined with others once additional markers are added to the map. This was confirmed by pulsed-field gel analysis; probes derived from 2 of the smallest linkage groups hybridized to two of the largest chromosome-sized bands, revealing a discrepancy between physical and genetic distance. The utility of the map was demonstrated by identifying molecular markers tightly linked to two genes of biological interest, mating type and avirulence. Bulked segregant analysis was used to identify additional molecular markers closely linked to these traits. This is the first genetic linkage map for any species in the genus Mycosphaerella or the family Mycosphaerellaceae.

Two genetic linkage maps of mungbean using RFLP and RAPD markers

2000 ◽  
Vol 51 (4) ◽  
pp. 415 ◽  
Author(s):  
C. J. Lambrides ◽  
R. J. Lawn ◽  
I. D. Godwin ◽  
J. Manners ◽  
B. C. Imrie
Keyword(s):  
Linkage Map ◽  
Segregation Distortion ◽  
Recombinant Inbred ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Rflp Markers ◽  
Linkage Maps ◽  
Linkage Groups ◽  
Genetic Linkage Maps ◽  
Map Distance

Two genetic linkage maps of mungbean derived from the cross Berken ACC 41 are reported. The F2 map constructed from 67 individuals consisted of 110 markers (52 RFLP and 56 RAPD) that grouped into 12 linkage groups. The linked markers spanned a total map distance of 758.3 cM. A recombinant inbred (RI) population derived from the 67 F2 individuals was used for the generation of an additional linkage map. The RI map, composed entirely of RAPD markers, consisted of 115 markers in 12 linkage groups. The linked markers spanned a total map distance of 691.7 cM. Using a framework set of RFLP markers, the F2 map was compared with another F2 mungbean map constructed in Minnesota. In general, the order of these markers was consistent between maps. Segregation distortion was observed for some markers. 14.5% (16/110) of mapped F2 markers and 24% (28/115) of mapped RI markers segregated with distorted ratios. Segregation distortion occurred in each successive generation after the F2 . The regions of distortion identified in the Australian maps did not coincide with regions of the Minnesota map.

Linkage of random amplified polymorphic DNA, isozyme and morphological markers in grasspea (Lathyrus sativus)

1999 ◽  
Vol 133 (4) ◽  
pp. 389-395 ◽  
Author(s):  
M. A. CHOWDHURY ◽  
A. E. SLINKARD
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Average Distance ◽  
Random Amplified Polymorphic Dna ◽  
Lathyrus Sativus ◽  
Morphological Markers ◽  
Linkage Studies ◽  
Linkage Groups

We constructed a genetic linkage map of grasspea (Lathyrus sativus L.; 2n = 14) from 100 F2 individuals derived from a cross between PI 426891.1.3 and PI 283564c.3.2. A total of 71 RAPD, three isozyme and one morphological markers segregated in the F2 progeny. A small fraction of markers (12%) deviated significantly from the expected Mendelian ratio (1[ratio ]2[ratio ]1 or 3[ratio ]1). Out of 75 markers, 69 (one morphological, three isozyme and 65 RAPD markers) were assigned to 14 linkage groups comprising 898 cM. The average distance between two adjacent markers was 17·2 cM. The present linkage map will serve as a reference point for further linkage studies in grasspea.

scholarly journals Construction of Genetic Map of Jute (Corchorus olitorius L.) Based on RAPD Markers

2009 ◽  
Vol 18 (2) ◽  
pp. 165-172 ◽  
Author(s):  
Samiul Haque ◽  
Nadim Ashraf ◽  
Selina Begum ◽  
R.H. Sarkar ◽  
Haseena Khan
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Group Analysis ◽  
Basic Chromosome Number ◽  
Marker Density ◽  
Linkage Groups ◽  
Average Marker Density ◽  
Rapd Polymorphism

The first and preliminary genetic linkage map of the jute genome was constructed with RAPD markers using two parents (variety O-9897 and accession no. 1805) and their F2 populations. Linkage analysis at a LOD (Log of odds base 10) score of 3.0 and a maximum distance 50 cM revealed 18 linkage groups. Among the 18 linkage groups, 15 contained single locus and the remaining three groups 16, 17 and 18 contained 2, 11 and 12 loci, respectively. The three multi locus linkage groups varying in length from 15.9 - 241.7 cM, snapped a total length of 463.7 cM with an average marker density of 19.6 cM between adjacent markers. The basic chromosome number of Corchorus spp. is seven (2n = 14), so in saturated map, seven linkage groups should have been obtained to represent the genome. But for linkage group analysis, the effort was very limited and the total number of loci (40) was also low.  Key words: Jute, Linkage map, RAPD, Polymorphism D.O.I 10.3329/ptcb.v18i2.3647 Plant Tissue Cult. & Biotech. 18(2): 165-172, 2008 (December)

Integration of Brassica A genome genetic linkage map between Brassica napus and B. rapa

Genome ◽  
2008 ◽  
Vol 51 (3) ◽  
pp. 169-176 ◽  
Author(s):  
Keita Suwabe ◽  
Colin Morgan ◽  
Ian Bancroft
Keyword(s):  
Molecular Markers ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Linkage Groups ◽  
Indel Markers ◽  
A Genome ◽  
High Level ◽  
High Degree ◽  
Genetic Integration

An integrated linkage map between B. napus and B. rapa was constructed based on a total of 44 common markers comprising 41 SSR (33 BRMS, 6 Saskatoon, and 2 BBSRC) and 3 SNP/indel markers. Between 3 and 7 common markers were mapped onto each of the linkage groups A1 to A10. The position and order of most common markers revealed a high level of colinearity between species, although two small regions on A4, A5, and A10 revealed apparent local inversions between them. These results indicate that the A genome of Brassica has retained a high degree of colinearity between species, despite each species having evolved independently after the integration of the A and C genomes in the amphidiploid state. Our results provide a genetic integration of the Brassica A genome between B. napus and B. rapa. As the analysis employed sequence-based molecular markers, the information will accelerate the exploitation of the B. rapa genome sequence for the improvement of oilseed rape.

scholarly journals Construction of a genetic linkage map in Lilium using a RIL mapping population based on SRAP marker

Genetika ◽  
2015 ◽  
Vol 47 (2) ◽  
pp. 425-438 ◽  
Author(s):  
Li-Jing Chen ◽  
Hong-Mei Li ◽  
Shao-Kun Sun ◽  
Muhammad Irfan ◽  
Jing-Wei Lin ◽  
...  
Keyword(s):  
Linkage Map ◽  
Recombinant Inbred ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Mapping Population ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Linkage Groups ◽  
Marker Interval ◽  
Interval Distance

A genetic linkage map of lily was constructed using RILs (recombinant inbred lines) population of 180 individuals. This mapping population was developed by crossing Raizan No.1 (Formolongo) and Gelria (Longiflomm) cultivars through single-seed descent (SSD). SRAPs were generated by using restriction enzymes EcoRI in combination with either MseI. The resulting products were separated by electrophoresis on 6% denaturing polyacrylamide gel and visualized by silver staining. The segregation of each marker and linkage analysis was done using the program Mapmaker3.0. With 50 primer pairs, a total of 189 parental polymorphic bands were detected and 78 were used for mapping. The total map length was 2,135.5 cM consisted of 16 linkage groups. The number of markers in the linkage groups varied from 1 to 12. The length of linkage groups was range from 11.2 cM to 425.9 cM and mean marker interval distance range from 9.4 cM to 345.4 cM individually. The mean marker interval distance between markers was 27.4 cM. The map developed in the present study was the first sequence-related amplified polymorphism markers map of lily constructed with recombinant inbred lines, it could be used for genetic mapping and molecular marker assisted breeding and quantitative trait locus mapping of Lilium.

scholarly journals GENETIC LINKAGE MAP FOR WATERMELON: SEGREGATION AND DISTRIBUTION OF DNA MARKERS

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 872a-872
Author(s):  
A. Levi ◽  
C. E. Thomas ◽  
J. Thies ◽  
A. Simmons ◽  
Y. Xu ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Genetic Distances ◽  
Aflp Markers ◽  
F2 Population ◽  
Skewed Segregation ◽  
Linkage Distance ◽  
Map Distance

Genetic linkage map is being constructed for watermelon based on a testcross population and an F2 population. About 51.0% and 31.8% of the markers in the testcross and F2 populations are skewed form the expected segregation ratios. AFLP markers appeared to be clustered on linkage regions, while ISSR and RAPD markers are randomly dispersed on the genome. AFLP markers also have greater genetic distances as compared with ISSR and RAPD markers, resulting in significant increase of map distance. An initial genetic map (based on the testcross population) that contains 27 ISSR and 141 RAPD markers has a total linkage distance of 1,166.2 cM. The addition of 2 ISSR, 8 RAPD and 77 AFLP markers increased the genetic distance of the map to 2,509.9 cM. Similar results with AFLP markers were also shown in mapping experiments with an F2S7 recombinant inbred line (RIL) population that was recently constructed for watermelon. Although the skewed segregation, marker order appeared to be consistent in linkage groups of the testcross and the F2 population. Experiments with SSR, and EST markers are being conducted to saturate the linkage map of watermelon genome.

A genetic linkage map for Arctic char (Salvelinus alpinus): evidence for higher recombination rates and segregation distortion in hybrid versus pure strain mapping parents

Genome ◽  
2004 ◽  
Vol 47 (2) ◽  
pp. 304-315 ◽  
Author(s):  
R A Woram ◽  
C McGowan ◽  
J A Stout ◽  
K Gharbi ◽  
M M Ferguson ◽  
...  
Keyword(s):  
Linkage Map ◽  
Segregation Distortion ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Salvelinus Alpinus ◽  
Linkage Groups ◽  
Recombination Rates ◽  
Pure Strain ◽  
Female Hybrid ◽  
Map Distance

We constructed a genetic linkage map for Arctic char (Salvelinus alpinus) using two backcrosses between genetically divergent strains. Forty-six linkage groups (expected = 39–41) and 19 homeologous affinities (expected = 25) were identified using 184 microsatellites, 129 amplified fragment length polymorphisms (AFLPs), 13 type I gene markers, and one phenotypic marker, SEX. Twenty-six markers remain unlinked. Female map distance (9.92 Morgans) was substantially higher than male map distance (3.90 Morgans) based on the most complete parental information (i.e., the F1 hybrids). Female recombination rates were often significantly higher than those of males across all pairwise comparisons within homologous chromosomal segments (average female to male ratios within families was 1.69:1). The female hybrid parent had significantly higher recombination rates than the pure strain female parent. Segregation distortion was detected in four linkage groups (4, 8, 13, 20) for both families. In family 3, only the largest fish were sampled for genotyping, suggesting that segregation distortion may represent regions possessing influences on growth. In family 2, almost all cases showing segregation distortion involved markers in the female hybrid parent.Key words: salmonid fishes, polyploidy, homeology, genetic markers.

scholarly journals (261) A PCR-based Linkage Map of Broccoli [Brassica oleracea(L.) var. italica] and Comparisons with Existing BrassicaMaps

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1090B-1090
Author(s):  
Allan Brown ◽  
Khalid Ibrahim ◽  
Sultana Islam ◽  
Elizabeth Jeffery ◽  
John Juvik
Keyword(s):  
Molecular Markers ◽  
Genetic Analysis ◽  
Linkage Map ◽  
Brassica Oleracea ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Morphological Markers ◽  
Linkage Groups ◽  
Interval Width ◽  
Brassica Oleracea L

A set of 216 PCR-based molecular markers was screened for polymorphisms using two morphologically dissimilar broccoli lines, `VI-158' and `BNC'. Fifty-nine of these markers, representing 69 detected polymorphisms and two morphological markers, were used to construct a genetic linkage map of broccoli [Brassicaoleracea (L.) var. italica] from a population of 162 F2:3 families generated from the cross between these two lines. Ten genetic linkage groups were generated that spanned a distance of 468 cM with an average interval width of 9.4 cm. This map represents the first combined SSR and SRAP map of Brassica oleracea. Comparisons are made to existing maps of Brassicanapus and to inter-specific maps of Brassicaoleracea. To our knowledge this is the first linkage map of broccoli [Brassicaoleracea (L.) var. italica] and should provide a useful tool for the genetic analysis of traits specific to the italica subspecies.

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