Cross-amplification and Characterization of Microsatellite Loci for the Genus Rhododendron

To assess cross-species amplification, we tested 38 microsatellite loci previously developed for Rhododendron delavayi Franch. and R. decorum Franch. in eight species representative of the genus Rhododendron. Sixteen pairs can be amplified successfully in all species, whereas two failed amplification in all species. Nine loci were polymorphic across six examined species with one to 11 alleles per locus. The observed and expected heterozygosity per locus varied from 0.07 to 0.65 and 0.44 to 0.81, respectively. Cross-application of these microsatellite loci will provide a potentially useful tool to investigate the genetic structure, gene flow, and evolutionary relationships in genus Rhododendron.

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Isolation and Characterization of Microsatellite Loci for the Ornamental Plant Primula obconica Hance (Primulaceae)

HortScience ◽

10.21273/hortsci.45.2.314 ◽

2010 ◽

Vol 45 (2) ◽

pp. 314-315 ◽

Cited By ~ 2

Author(s):

Hai-fei Yan ◽

Xue-jun Ge ◽

Chi-ming Hu ◽

Gang Hao

Keyword(s):

Genetic Variation ◽

Genetic Structure ◽

Microsatellite Markers ◽

Microsatellite Loci ◽

Ornamental Plant ◽

Microsatellite Variation ◽

Isolation And Characterization ◽

Expected Heterozygosity ◽

Fiasco Protocol

Nine microsatellite loci were isolated from Primula obconica using the FIASCO protocol. We used 30 individuals from three populations for the assessment of microsatellite variation. Seven loci were detected with microsatellite polymorphism. The number of alleles per locus ranged from three to seven. The average observed heterozygosity and expected heterozygosity ranged from 0.167 to 0.6 and from 0.409 to 0.653, respectively. These microsatellite markers will be useful to assess the genetic variation and genetic structure of P. obconica.

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Isolation and Characterization of 10 Polymorphic Microsatellite Loci in Paphiopedilum concolor (Batem.) Pfitzer (Orchidaceae) and Cross-species Amplification

HortScience ◽

10.21273/hortsci.45.8.1286 ◽

2010 ◽

Vol 45 (8) ◽

pp. 1286-1287 ◽

Cited By ~ 1

Author(s):

Long-na Li ◽

Songjun Zeng ◽

Feng Zheng ◽

Zhi-lin Chen ◽

Kun-lin Wu ◽

...

Keyword(s):

Genetic Structure ◽

Microsatellite Locus ◽

Microsatellite Loci ◽

Genomic Library ◽

Enriched Genomic Library ◽

Relationship Patterns ◽

Isolation And Characterization ◽

Expected Heterozygosity ◽

Polymorphic Microsatellite

Ten polymorphic microsatellite loci were isolated and characterized from an enriched genomic library of Paphiopedilum concolor (Batem.) Pfitzer. The number of alleles per microsatellite locus ranged from three to 11 with an average of 6.4 in a sample of 30 individuals from three populations. The observed and expected heterozygosity ranged from 0.200 to 0.800 and from 0.544 to 0.827, respectively. These microsatellites can be used as tools to investigate the genetic structure of P. concolor populations and relationship patterns with closely related taxa.

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Development and Characterization of 15 Microsatellite Loci for Rhododendron delavayi Franch. (Ericaceae)

HortScience ◽

10.21273/hortsci.45.3.457 ◽

2010 ◽

Vol 45 (3) ◽

pp. 457-459 ◽

Cited By ~ 4

Author(s):

Nian Wang ◽

Zhang Chang Qin ◽

Jun-bo Yang ◽

Jing-li Zhang

Keyword(s):

Gene Flow ◽

Genetic Structure ◽

Microsatellite Loci ◽

Sympatric Species ◽

Ornamental Plant ◽

Natural Hybridization ◽

Congeneric Species ◽

Allele Number ◽

Species Study

Rhododendron delavayi Franch. is an important ornamental plant and often plays a role in natural hybridization with other sympatric species in Rhododendron subgenus Hymenanthes. Fifteen microsatellite loci were developed and characterized in this species. The average allele number of these microsatellites was four per locus, ranging from three to six. The ranges of expected (HE) and observed (HO) heterozygosities were 0.0365 to 0.7091 and 0.0263 to 0.9512, respectively. Cross-species amplification in R. agastum and R. decorum showed that a subset of these markers holds promise for congeneric species study. These sets of markers are potentially useful to investigate the genetic structure and gene flow of R. delavayi and other congeneric species.

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