scholarly journals Increasing In Vitro Shoot Elongation and Proliferation of ‘G.30’ and ‘G.41’ Apple by Chilling Explants and Plant Growth Regulators

HortScience ◽  
2016 ◽  
Vol 51 (7) ◽  
pp. 899-904 ◽  
Author(s):  
Fang Geng ◽  
Renae Moran ◽  
Michael Day ◽  
William Halteman ◽  
Donglin Zhang
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Growth ◽  
Shoot Elongation ◽  
Shoot Length ◽  
Optimum Concentration ◽  
Shoot Number ◽  
Collection Date

These studies were conducted to determine the most effective methods for increasing shoot elongation during the initial proliferation stage of micropropagation in two dwarfing apple, Malus ×domestica (Borkh.), rootstock cultivars. Several experiments were conducted to compare explant collection date, exposure to chilling (5 ± 1 °C) temperatures, and varying concentrations of plant growth regulators in Murashige and Skoog (MS) media. Microshoot growth of ‘Geneva 41’ (‘G.41’) was very low and unaffected by chilling duration from 0 to 8 weeks or by gibberellic acid (GA3) concentration from 0 to 1.0 mg·L−1, but was improved by an additional subculture which increased shoot length from 1 to 15 mm. In ‘Geneva 30’ (‘G.30’), shoot elongation was most affected by date, chilling explants, and by optimizing cytokinin concentration and type. Explant collection date in April increased shoot growth compared with August or November. Microshoot growth of ‘G.30’ was increased by chilling nodal explants for 4 and 6 weeks when explants were collected in August and November, but not in April. Eight weeks chilling was detrimental for explants collected in April, and generally had little or no effect with August and November. The cytokinin 6-benzylaminopurine (BA) increased shoot number to a greater extent than thidiazuron (TDZ) or zeatin (ZT), and was also more effective for increasing shoot elongation with concentrations of 0 to 2.0 mg·L−1. In ‘G.30’, GA3 increased shoot growth at the optimum concentration of BA, but not with lower concentrations. ‘G.30’ microshoots were fewer and shorter with 24-epi-brassinolide (EBR) at concentrations of 0.1 and 1.0 mg·L−1. Chemical names: N-phenyl-N’-(1,2,3-thiadiazol-5-yl)urea (TDZ), 6-(4-hydroxy-3-methylbut-2-enylamino)purine (ZT).

scholarly journals The Response of in Vitro Propagation of Marumi Kumquat (fortunella Japonica Thunb.) to Different Culture Media, Plant Growth Regulators And Different Fructose Concentration

2020 ◽  
Vol 23 (1) ◽  
pp. 178-190
Author(s):  
Jeillan Hussein ◽  
Diaa ibraheam
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Nutritional Value ◽  
Culture Media ◽  
Shoot Length ◽  
Ms Medium ◽  
Surface Sterilization

Marumi kumquat (Fortunella Japonica) is culture for its valuable nutritional value and medicinal importance in many regions of the world. The current study aimed to evaluate the effect of two types of media enriched with different concentrations of fructose and different plant growth regulators and different fructose concentration on in vitro propagation of Fortunella Japonica. The findings showed that the most effective treatment for explant surface sterilization was by using 0.1% HgCl2 for ten minutes which give best results for production contamination-free explants at the initiation cultures. At multiplication stage, WPM medium gave better results at all tested BA levels as compared with MS medium. No significant differences were showed by using BA alone or in combination with GA3 in the measured parameters. It has been observed that WPM medium supplemented with 0.5mgl-1 BA with the presence of 30mgl-1 fructose was able to give the highest shoot length (1.56cm) with maximum shoots number/explant 9.0 and highest leaves number/explant (21.0). The proliferated shoots were exposed to full strength MS medium salts supplemented with 2mgl-1 NAA which showed the highest ratio of rooting. In vitro rooted plantlets were gradually acclimatized and transferred to open air conditions, which recorded a high survive rate reached to 92%

scholarly journals Evaluation of In Vitro Shoot Elongation and Rooting of Date Palm, and Determination of Physiological Characteristics of Regenerated Plantlets

2019 ◽  
Vol 11 (1) ◽  
pp. 77-85 ◽  
Author(s):  
Reda MEZIANI ◽  
Mouaad Amine MAZRI ◽  
Mahassine ARHAZZAL ◽  
Ilham BELKOURA ◽  
Chakib ALEM ◽  
...  
Keyword(s):  
Survival Rate ◽  
Stomatal Conductance ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Culture Medium ◽  
Date Palm ◽  
Shoot Elongation ◽  
Physiological Characteristics

The effects of various culture conditions on shoot elongation, rooting and plantlet acclimatization were tested. Adventitious shoots obtained through direct organogenesis of date palm (Phoenix dactylifera L.) cv. ‘Mejhoul’ were used as explants. The effects of culture medium texture, plant growth regulators, polyvinylpyrrolidone, adenine, myo-inositol, L-glutamine, and carbon source on in vitro plantlet quality and subsequent acclimatization were evaluated. The most effective culture medium was the semi-solid and half-strength Murashige and Skoog medium without plant growth regulators, supplemented with 30 g L-1 sucrose. After 3 months of culture on this medium, the average shoot length was 13.6 cm, the average number of adventitious roots per shoot was 3.6, and the average root length was 3.85 cm. The survival rate of these plantlets in acclimatization was 90%. On the other hand, liquid medium, plant growth regulators, polyvinylpyrrolidone, adenine, myo-inositol and L-glutamine did not increase the survival rate during acclimatization. Along with these experiments, some physiological characteristics of the plantlets obtained in vitro were also determined. Chlorophyll content and fluorescence, foliar surface and stomatal conductance were measured after 3 months of culture in each medium. The ranges were as follows: Chlorophyll content, 11.7-31.8 CCI; chlorophyll fluorescence, 0.633-0.795; foliar surface, 7.35-13.29 cm2; and stomatal conductance, 10.3-36.0 mmol m-2 s-1. Interestingly, positive correlations between the physiological characteristics of the plantlets and their survival percentage in the glasshouse were revealed. The findings of this investigation will be valuable for large-scale and cost-saving production of date palm cv. ‘Mejhoul’ plants.

scholarly journals Evaluation of Genetic Stability and Effects of Plant Growth Regulators for in vitro Propagation of Underutilized Vitis amurensis ‘Cheongsan’

2019 ◽  
Vol 47 (3) ◽  
pp. 987-994
Author(s):  
Joon-Ho KWON ◽  
Young-Sik PARK ◽  
Si-Hong KIM ◽  
Jae-Yun HEO
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Shoot Growth ◽  
Shoot Multiplication ◽  
Vitis Amurensis ◽  
Root Induction ◽  
Propagation Methods

Vitis amurensis ‘Cheongsan’ is a plant with high economic value in both medical and agricultural applications. However, its utilization has been restricted owing to difficulties encountered when applying traditional mass propagation methods, requiring instead application of in vitro propagation methods for their mass scale production. Hence, this study was conducted to find the optimal plant growth regulators for shoot multiplication and root induction during in vitro propagation. Among the three cytokinins used at multiple concentrations for culture initiation and shoot multiplication, the most positive response was found with MS medium containing 5.0 μM 6-benzyladenine (BA), compared to more modest responses from other types of cytokinin, such as kinetin (KIN) and thidiazuron (TDZ). For root induction, medium supplemented with α-naphthaleneacetic acid (NAA) produced a callus and inhibited shoot growth in explants, whereas indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) did not create any significant problems, but did display differences in root induction efficiencies. Generally, root induction responses with IBA were better that those with IAA. The maximum rooting rates were observed without callus formation and no shoot growth inhibition from explants grown on media supplemented with 0.67 μM IBA. Further, inter-simple sequence repeat (ISSR) analyses revealed that micropropagated plantlets generated in medium supplemented with 5.0 μM BA and 0.67 μM IBA did not lead to genetic variation. Therefore, the application of the in vitro propagation method developed in this study could be used on a commercial scale and will offer opportunities to strengthen the industrial use of V. amurensis ‘Cheongsan’.

scholarly journals In Vitro Propagation Of ‘Gisela 5’ Rootstock As Affected By Mineral Composition Of Media And Plant Growth Regulators

2015 ◽  
Vol 23 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Maliheh Fallahpour ◽  
Seied Mehdi Miri ◽  
Naser Bouzari
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Mineral Composition ◽  
Growth Regulators ◽  
Sweet Cherry ◽  
Culture Media ◽  
Shoot Multiplication ◽  
Shoot Number ◽  
Gisela 5

AbstractThe purpose of the presented study was to determine the best mineral composition of media and plant growth regulators in the micropropagation of the ‘Gisela 5’ (Prunus cerasus×P.canescens) dwarf sweet cherry rootstock. Nodal explants from young healthy shoots were excised and cultured on Murashige and Skoog medium without growth regulators.In vitroraised shoot tips were transferred to three culture media including Murashige and Skoog (MS), Driver and Kuniyuki (DKW) and Lloyd and McCown (WPM) containing benzyl adenine (BA) (0.5, 1 or 2 mg·l−1) in combination with kinetin (Kin) at 0 or 0.5 mg·l−1. WPM and DKW media were proving to be the most effective, resulting in a higher percentage of shoot multiplication and shoot number as compared to MS. BA in concentration 2 mg·l−1resulted in the highest number of microshoots per explant (3.1). For rooting, 0, 0.5, 1 or 2 mg·l−1indole-3-butyric acid (IBA) on MS, DKW and WPM media were tested. WPM medium containing 2 mg·l−1IBA was most effective for rooting (93.7%) in comparison to MS (53.1%) and DKW (14.0%). Rooted plantlets were successfully hardened and established in pots.

scholarly journals Multiplicação in vitro de Caesalpinia pyramidalis (Leguminosae)

2013 ◽  
Vol 13 ◽  
Author(s):  
Tecla Dos Santos Silva ◽  
Cristina Ferreira Nepomuceno ◽  
Bárbara Paula dos Santos Borges ◽  
Bruno Freitas Matos Alvim ◽  
José Raniere Ferreira De Santana
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Culture Medium ◽  
Dry Mass ◽  
Shoot Length ◽  
Nodal Segments ◽  
In Vitro Multiplication ◽  
Different Types

Caesalpinia pyramidalis is a species endemic to the Caatinga and known popularly as catingueira, which is widely used by local people, mainly for its timber and medicinal and fodder properties. This study investigated the effects of different types and concentrations of plant growth regulators on the in vitro multiplication of C. pyramidalis. In the first experiment, nodal segments were inoculated in media containing different combinations (0.0–8.0 µM) of BAP and NAA. In the second experiment, nodal segments wereinoculated in media containing different types (KIN, BAP and TDZ) and concentrations (0.0–16μM) of cytokinins. We used a WPM medium supplemented with 87.64 mM sucrose and solidified with 7.0 g L-1 agar. After 45 days, the highest number of shoots, leaf number, shoot length and dry mass of shoots were obtained when nodal segments were inoculated into a culture medium without plant growth regulators.

scholarly journals Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Zingiber Officinale Roscoe ◽  
Planting Materials ◽  
Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

scholarly journals The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis

2011 ◽  
Vol 3 (3) ◽  
pp. 97-100
Author(s):  
Naimeh SHARIFMOGHADAM ◽  
Abbas SAFARNEJAD ◽  
Sayed Mohammad TABATABAEI
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Base Material ◽  
Culture Technique ◽  
Induction Medium ◽  
Root Induction ◽  
Amygdalus Communis

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

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