scholarly journals An Improved Method to Track Changes of Candidatus Liberibacter asiaticus Titer in HLB-affected Citrus Trees

HortScience ◽  
2019 ◽  
Vol 54 (8) ◽  
pp. 1357-1360 ◽  
Author(s):  
Ed Etxeberria ◽  
Pedro Gonzalez ◽  
Ariel Singerman ◽  
Timothy Ebert
Keyword(s):  
Sampling Method ◽  
Sampling Period ◽  
Tree Canopy ◽  
Candidatus Liberibacter Asiaticus ◽  
Improved Method ◽  
Destructive Sampling ◽  
Candidatus Liberibacter ◽  
Random Variability ◽  
Liberibacter Asiaticus ◽  
Citrus Trees

Monitoring the health of Huanglongbing-affected citrus trees by following changes in leaf Candidatus Liberibacter asiaticus (CLas) titer has an inherent element of imprecision because CLas titer varies considerably within the tree canopy and with calendar seasons. In addition, the destructive sampling method used to determine CLas titer entails a different set of leaves per sampling period adding to the inconsistency and inexactitude of the results. To overcome these ambiguities and to reduce the numerical variability between samples, we developed an experimental method that analyzes portions of the same treated leaves for up to four sampling periods. By assaying subsamples of adjacent locations of the same leaf, random variability was significantly reduced, and comparative analysis can be carried out with greater precision.

scholarly journals Distribution of Candidatus Liberibacter asiaticus in Citrus and the Asian Citrus Psyllid in Texas Over a Decade

Plant Disease ◽  
2020 ◽  
Vol 104 (4) ◽  
pp. 1118-1126 ◽  
Author(s):  
Mamoudou Sétamou ◽  
Olufemi J. Alabi ◽  
Madhurababu Kunta ◽  
Jon Dale ◽  
John V. da Graça
Keyword(s):  
Leaf Tissue ◽  
The United States ◽  
Asian Citrus Psyllid ◽  
Candidatus Liberibacter Asiaticus ◽  
Residential Areas ◽  
Survey Period ◽  
Tissue Samples ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
Citrus Trees

Huanglongbing (HLB, citrus greening disease) in the major citrus-producing states of the United States is associated with Candidatus Liberibacter asiaticus (CLas), which is vectored by the Asian citrus psyllid (ACP). Surveys were conducted in Texas from 2007 to 2017 to assess the prevalence and titer of CLas in ACPs and citrus trees. ACP and citrus leaf tissue samples were collected from suspect trees in residential areas and commercial groves (orchards) and assayed for CLas by quantitative PCR. CLas detection in ACPs (2011) preceded that of citrus trees (2012) by several months. Annual incidences of CLas-positive ACPs and leaf tissue followed an exponential growth pattern over the survey period, varying from 0.03 to 28.7% in ACPs and 0.6 to 36.5% in citrus trees. There was a significant and positive relationship between the monthly incidences of CLas-positive ACP and leaf tissue samples. The proportion of HLB detection sites also increased with time, reaching 26 and 40% of commercial groves and residential sites, respectively, by 2017. Seasonal variations were observed in the incidences of CLas-positive ACPs and citrus trees such that significantly more CLas-positive ACPs and trees were recorded during the fall and winter of a given year relative to the hot summer. A temporal analysis of the class distribution of cycle threshold values revealed a trend of increased bacterial accumulation in ACPs and trees over time, with the trend more pronounced for the former than the latter host type. These findings provide a comprehensive insight into the ongoing CLas/HLB epidemic in Texas, with potential lessons for California and other citrus-producing areas where the disease is not yet established.

Detection of Candidatus Liberibacter asiaticus from branch collars of citrus trees

2016 ◽  
Vol 82 (5) ◽  
pp. 248-253
Author(s):  
Yoshihiro Nakanishi ◽  
Ken Takesaki ◽  
Katsuhiko Miyaji ◽  
Hiroaki Kitazawa
Keyword(s):  
Candidatus Liberibacter Asiaticus ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
Citrus Trees

Seasonal variation of Candidatus Liberibacter asiaticus population in Citrus trees in southeast of Iran

2021 ◽  
Vol 159 (4) ◽  
pp. 799-809
Author(s):  
Atiyeh Bahari ◽  
Seyed Mehdi Alavi ◽  
Esmail Saberi ◽  
Mehdi Azadvar ◽  
Masoud Shams-Bakhsh
Keyword(s):  
Seasonal Variation ◽  
Candidatus Liberibacter Asiaticus ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
Citrus Trees
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