diaphorina citri
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2022 ◽  
Author(s):  
Zhong‐Zhen Wu ◽  
Wan‐Ying Zhang ◽  
Yi‐Zhu Lin ◽  
Da‐Qi Li ◽  
Ben‐Shui Shu ◽  
...  

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Surendra Neupane ◽  
Sylvia I. Bonilla ◽  
Andrew M. Manalo ◽  
Kirsten S. Pelz-Stelinski

AbstractWolbachia, a gram-negative $$\mathrm{\alpha }$$ α -proteobacterium, is an endosymbiont found in some arthropods and nematodes. Diaphorina citri Kuwayama, the vector of ‘Candidatus Liberibacter asiaticus’ (CLas), are naturally infected with a strain of Wolbachia (wDi), which has been shown to colocalize with the bacteria pathogens CLas, the pathogen associated with huanglongbing (HLB) disease of citrus. The relationship between wDi and CLas is poorly understood in part because the complete genome of wDi has not been available. Using high-quality long-read PacBio circular consensus sequences, we present the largest complete circular wDi genome among supergroup-B members. The assembled circular chromosome is 1.52 megabases with 95.7% genome completeness with contamination of 1.45%, as assessed by checkM. We identified Insertion Sequences (ISs) and prophage genes scattered throughout the genomes. The proteins were annotated using Pfam, eggNOG, and COG that assigned unique domains and functions. The wDi genome was compared with previously sequenced Wolbachia genomes using pangenome and phylogenetic analyses. The availability of a complete circular chromosome of wDi will facilitate understanding of its role within the insect vector, which may assist in developing tools for disease management. This information also provides a baseline for understanding phylogenetic relationships among Wolbachia of other insect vectors.


2022 ◽  
pp. 105907
Author(s):  
Francesc Gomez-Marco ◽  
Marco Gebiola ◽  
Gregory S. Simmons ◽  
Richard Stouthamer
Keyword(s):  

Plant Disease ◽  
2021 ◽  
Author(s):  
Xuejin Cui ◽  
Kehong Liu ◽  
Jie Huang ◽  
Shimin Fu ◽  
Qingdong Chen ◽  
...  

Citrus Huanglongbing (HLB) is present in 10 provinces in China and is associated with “Candidatus Liberibacter asiaticus” (CLas), which is transmitted by the Asian citrus psyllid (Diaphorina citri, ACP). To date, HLB and ACP have expanded to Yibin city of Sichuan Province, posing an imminent threat to the citrus belt of upper and middle reach of Yangtze River, an important late maturing citrus-producing area in China. To understand the epidemiological route of CLas and ACP in newly invaded regions of Sichuan and thereby better establish an HLB-interception zone ranging from Leibo to Yibin, we evaluated the molecular variability of 19 CLas draft genomes from citrus or dodder (Cuscuta campestris). They include three type-specific prophage loci, three variable numbers of tandem repeat (VNTR) loci, a miniature inverted-repeat transposable element (MITE) types, and population diversity of 44 ACP mitochondrial genomes. The results indicated that CLas isolates in the newly invaded area (Pingshan) were more diverse than those in the HLB endemic areas (Leibo and Ningnan). Phylogenetic analysis based on mitochondrial genomes demonstrated that ACPs in Leibo, Pingshan and Xuzhou (rural areas) represent a new group (MG4), distinguished by the three unique SNPs in cox1, nad4 and cytb. However, the ACPs sampled from the urban areas of Cuiping and Xuzhou belonged to the southeastern China group (MG2-1). Altogether, our study revealed multiple sources of ACP and CLas in the HLB-interception zone and proposed their transmission route. This study contributes to the formulation of precise HLB prevention and control strategies in the HLB-interception zone in Sichuan and could be useful for HLB management efforts in other regions.


2021 ◽  
Vol 1 (1) ◽  
pp. 644-651
Author(s):  
Mofit Eko Poerwanto

Citrus production in Indonesia and in the world is suffered from disease of citrus vein phloem degeneration (CVPD. It was vectored by psylids (Diaphorina citri). The psylids used citrus plant volatiles as cues for finding their host plants for feeding and laying eggs. Extract of guava leaves was a prospective control material for declaining population of psyllid. Investigation by research was conducted to determine the effect of CVPD symptomatic citrus plants in attracting psylids gravid female for staying and laying eggs, and also to determine the repellency ability of guava shoots to adult psyllids. Citrus buds of healthy plants and CVPD symptomatic plants were exposed to ten gravid female and the number of psylids stay and the number of eggs per bud was recorded. Y-tube olfactometer was used to determine repellent effect of upper shoot, middle shoot, and bottom shoot of guava leave to ten adult psyllids. The result shows that CVPD symptomatic plants was more attractive for laying eggs, eventhough the number of eggs was higher on healthy plants. Repellence effect to psyllids adult was identified in guava leave extracts. The increase of leaf age would decrease the effect. It was suggested that repellent properties was highest youngest leaf or upper shoots of guava.


2021 ◽  
Vol 47 (4) ◽  
pp. 723-732
Author(s):  
José Mario Miranda-Ramírez ◽  
Catarino Perales-Segovia ◽  
Mario Alberto Miranda-Salcedo ◽  
Diana Miranda-Medina

Se evaluaron insecticidas comerciales de bajo impacto ambiental para el control de Diaphorina citri en el cultivo de limón mexicano en el Valle de Apatzingán, Michoacán, México. Se utilizó un diseño completamente al azar con doce tratamientos (insecticidas) y diez repeticiones, la unidad experimental fue un árbol. La técnica para la recolección de datos en campo fue el “muestreo por golpeteo”. La toma de datos se realizó en premuestreo como punto de inicio y comparación y después se llevaron a cabo los muestreos a los 3, 6, 12, 20, 26, 35 y 41 días. Para el análisis de datos se empleó la prueba Post Hoc de homogeneidad de datos de las varianzas, ANDEVA y la prueba de comparación de medias de Tukey (P≤ 0,05). Se observó que el Tolfenpyrad, mantuvo una efectividad constante durante todos los muestreos, al reducir la población de psílidos en un 95,65 %. El Pyrifluquinazon al inicio disminuyó de 4,9 en pre muestro a 0,6 psílidos a los 3 días después de la aplicación, equivalente a un 87,75% de reducción. El Fenpyroximato, mostró ser un insecticida eficaz, al presentar un rango de reducción de la población de psílidos de 40,32 a 83,87% en todos los muestreos. Se concluye que, el Tolfenpyrad y el Fenpyroximato son moléculas químicas de nueva generación que evidencian una mortalidad de adultos del psílido alta, por lo que son una alternativa viable para el manejo de la plaga en la región del Valle de Apatzingán, Michoacán.


Author(s):  
Luis Carlos Ramos Aguila ◽  
Komivi Senyo Akutse ◽  
Bamisope Steve Bamisile ◽  
Jessica Paola Sánchez Moreano ◽  
Hafiza Javaria Ashraf ◽  
...  

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2476
Author(s):  
Mubasher Hussain ◽  
Pasco B. Avery ◽  
Wenchao Zhu ◽  
Marco Pitino ◽  
Steven P. Arthurs ◽  
...  

Entomopathogenic fungi are proposed biological control agents against the Asian citrus psyllid (Diaphorina citri). We quantified the pathogenicity of Cordyceps javanica strain Apopka 97 strain (Cja Apopka 97) (blastospores and conidia) against D. citri using the spray technique. We also used light and scanning electron microscopy to observe the Cja Apopka 97, infection process against D. citri adults at different stages pre- and post-mortem. Our findings demonstrated that psyllid mortality in the blastospore treatment ranged from 8 to 25% within 24–48 h of exposure, compared to 0% in the conidial and control treatments. However, psyllid mortality rate had reached 100% by 7 days after exposure at a concentration of 107 spores/mL, under both fungal treatments compared to the controls (0%). SEM and light microscopy revealed several stages in in the Cja Apopka 97 infection process of D. citri, including spore adherence and germ tube formation within 24 h post-inoculation, penetration pegs and mycelia growth on wings after 72 h, rupturing of cuticle after 96 h and mycelial mass colonizing host body after 144 h. Our study findings provide basic information on the interaction of entomopathogenic fungi with D. citri which will assist in the understanding of the infection process and the potential roles of entomopathogenic fungi in its management.


Insects ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1074
Author(s):  
Jian-Chun Song ◽  
Zhan-Jun Lu ◽  
Long Yi ◽  
Hai-Zhong Yu

Trehalose-6-phosphate synthase (TPS) plays an important role in the synthesis of trehalose. In the current study, a TPS gene was obtained from Diaphorina citri, and named as DcTPS1 which encoded a protein of 833 amino acid residues. Real-time quantitative PCR (qPCR) analysis revealed that DcTPS1 had the highest expression level in the midgut and fifth-instar nymph stage. Knockdown of DcTPS1 by RNA interference (RNAi) induced an abnormal phenotype and increased mortality and malformation rate with a decreased molting rate. In addition, silencing of DcTPS1 significantly inhibited D. citri chitin metabolism and fatty acid metabolism, while the expression levels of fatty acid decomposition-related genes were downregulated. Furthermore, comparative transcriptomics analysis revealed that 791 differentially expressed genes (DEGs) were upregulated and 678 DEGs were downregulated when comparing dsDcTPS1 groups with dsGFP groups. Bioinformatics analysis showed that upregulated DEGs were mainly involved in oxidative phosphorylation, whereas downregulated DEGs were mainly attributed to the lysosome and ribosome. These results indicated that DcTPS1 played an important role in the growth and development of D. citri.


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