scholarly journals Characterization of Aspergillus niger B-1 RNase and Its Inhibitory Effect on Pollen Germination and Pollen Tube Growth in Selected Tree Fruit

2000 ◽  
Vol 125 (1) ◽  
pp. 9-14 ◽  
Author(s):  
Levava Roiz ◽  
Uzi Ozeri ◽  
Raphael Goren ◽  
Oded Shoseyov
Keyword(s):  
Aspergillus Niger ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Field Experiments ◽  
Prunus Persica ◽  
Inhibitory Effect ◽  
Tube Growth

Aspergillus niger B-1 (CMI CC 324626) extracellular RNase (RNase B1) was purified to homogeneity. It was found to contain two isoforms of 32- and 40-kDa glycoproteins, sharing a 29-kDa protein moiety. Optimal RNase activity was observed at 60 °C and pH 3.5. In `Almog' peach [Prunus persica (L.) Batsch (Peach Group) `Almog'] and `Murcott' tangerine (Citrus reticulata Blanco `Murcott') the enzyme inhibited pollen germination and pollen tube growth in vitro as well as in vivo. In field experiments, spray application of the RNase caused a reduction in `Fantasia' nectarine [Prunus persica (L.) Batsch (Nectarine Group) `Fantasia'] fruit set and interfered with embryo development. The biological effect of the RNase may be of horticultural value, due to its potential to control fertilization.

In Vitro Germination of Eucalyptus Pollen: Response to Variation in Boric Acid and Sucrose

1989 ◽  
Vol 37 (5) ◽  
pp. 429 ◽  
Author(s):  
BM Potts ◽  
JB Marsden-Smedley
Keyword(s):  
Pollen Tube ◽  
Boric Acid ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Sucrose Concentration ◽  
Response Surfaces ◽  
Tube Growth ◽  
Pollen Competition

The effect of boric acid (0-450 ppm) and sucrose (0-40%) on pollen germination and pollen tube growth in Eucalyptus globulus, E. morrisbyi, E. ovata and E. tirnigera was examined in vitro. Over the con- centrations tested, sucrose had by far the largest effect upon both pollen germination and tube lengths. The optimum sucrose concentration for pollen germination (30%) and pollen tube growth (20%) differed markedly with very little (<lo%) germination occurring in the absence of sucrose. The interaction of sucrose and boric acid was significant. However, in general both pollen germination and pollen tube growth were increased by the addition of up to 100 ppm boric acid, but above this level the response plateauxed. The four species differed significantly in their pattern of response to both boric acid and sucrose and the predicted optima derived from analysis of response surfaces differed between species. The predicted sucrose concentration for optimal germination and growth of E. urnigera pollen was consistently less than the other species and in terms of the optimal level of boric acid for pollen tube growth species can be ranked in the order E. globulus > E. ovata > E. morrisbyi = E. urnigera. Pollen germination and tube growth of all four species on a medium comprising 20% sucrose and 200 ppm boric acid would not differ significantly from the observed maximum response of each species and this could suffice as a generalised medium. However, if only percentage germination is to be assessed 30% sucrose would be preferable. It is argued that subtle interspecific differences in optimal in vitro con- ditions for pollen germination and pollen tube growth are likely to reflect differences in pollen physiology which in vivo may have important implications for the success of hybridisation where pollen competition occurs.

scholarly journals Pollen germination and pollen tube growth in ZP maize lines

Genetika ◽  
2014 ◽  
Vol 46 (3) ◽  
pp. 935-948 ◽  
Author(s):  
Radosav Cerovic ◽  
Zorica Pajic ◽  
Milomir Filipovic ◽  
Milica Fotiric-Aksic ◽  
Sanja Radicevic ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Growth Dynamics ◽  
Tube Growth ◽  
Open Pollination ◽  
Successful Management ◽  
Parental Lines

The study was conducted on the in vitro pollen germination at 26?, 28?, 32? and 35?C for 24h of male parental lines, pollen tube growth in vivo in cross pollination of female and male parental lines that make couples in four hybrids: ZP 504 su (? ZPPL 51 ? ? ZPPL 67); ZP 677 (? ZPPL 17 ? ? ZPPL 201); ZP 704 (? ZPPL 109 ? ? ZPPL 79), ZP 611 k (? ZPPL 126 ? ? ZPPL 105), and the open pollination of female parental lines of the above mentioned hybrids. Pollen germination in vitro and pollen tube growth dynamics in vivo showed different genotypic specificities with the tests applied. The obtained results were discussed in the context of reproductive biology of ZP maize lines and aimed to create the preconditions for successful management and direction of the process in practice - seed production in certain environmental conditions.

scholarly journals Pollen Germination and Pollen Tube Growth of Arabidopsis thaliana: in vitro and Semi in vivo Methods

BIO-PROTOCOL ◽  
2018 ◽  
Vol 8 (16) ◽  
Author(s):  
Hugh Dickinson ◽  
Josefina Rodriguez-Enriquez ◽  
Robert Grant-Downton
Keyword(s):  
Arabidopsis Thaliana ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Tube Growth

scholarly journals Hyperoside promotes pollen tube growth by regulating the depolymerization effect of actin-depolymerizing factor 1 on microfilaments in okra

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Biying Dong ◽  
Qing Yang ◽  
Zhihua Song ◽  
Lili Niu ◽  
Hongyan Cao ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Actin Depolymerizing Factor ◽  
Specific Mechanism ◽  
Promoting Effect ◽  
New Research

AbstractMature pollen germinates rapidly on the stigma, extending its pollen tube to deliver sperm cells to the ovule for fertilization. The success of this process is an important factor that limits output. The flavonoid content increased significantly during pollen germination and pollen tube growth, which suggests it may play an important role in these processes. However, the specific mechanism of this involvement has been little researched. Our previous research found that hyperoside can prolong the flowering period of Abelmoschus esculentus (okra), but its specific mechanism is still unclear. Therefore, in this study, we focused on the effect of hyperoside in regulating the actin-depolymerizing factor (ADF), which further affects the germination and growth of pollen. We found that hyperoside can prolong the effective pollination period of okra by 2–3-fold and promote the growth of pollen tubes in the style. Then, we used Nicotiana benthamiana cells as a research system and found that hyperoside accelerates the depolymerization of intercellular microfilaments. Hyperoside can promote pollen germination and pollen tube elongation in vitro. Moreover, AeADF1 was identified out of all AeADF genes as being highly expressed in pollen tubes in response to hyperoside. In addition, hyperoside promoted AeADF1-mediated microfilament dissipation according to microfilament severing experiments in vitro. In the pollen tube, the gene expression of AeADF1 was reduced to 1/5 by oligonucleotide transfection. The decrease in the expression level of AeADF1 partially reduced the promoting effect of hyperoside on pollen germination and pollen tube growth. This research provides new research directions for flavonoids in reproductive development.

Pollen–pistil interaction, pistil histology and seed production in A×B grain sorghum crosses under chilling field temperatures

2009 ◽  
Vol 148 (1) ◽  
pp. 73-82 ◽  
Author(s):  
M. E. CISNEROS-LÓPEZ ◽  
L. E. MENDOZA-ONOFRE ◽  
H. A. ZAVALETA-MANCERA ◽  
V. A. GONZÁLEZ-HERNÁNDEZ ◽  
G. MORA-AGUILERA ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Seed Production ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Vascular Tissue ◽  
Pollen Viability ◽  
Tube Growth ◽  
Male Sterile ◽  
Pollen Diameter

SUMMARYSix pairs of isogenic lines of sorghum (Sorghum bicolor L. Moench) were sown in field plots in Montecillo, State of México (2240 m altitude), in 2005 and 2006. Crosses A (♀)×B (♂) were done in each pair. In A-lines, the length of pistil, stigma, style and ovary, as well as the ovary width, were measured. In B-lines, pollen diameter, viability (cytoplasm density) and production were evaluated. Pollen germination and pollen tube growth in the pistils of the A-lines, were quantified in vivo with aniline blue and epifluorescence 18 h after pollination (HAP), while fertilized pistils were counted at 96 HAP. Histological studies on both pollinated and non-pollinated pistils were performed in one male-sterile line. Seed yield, mean-seed weight, seeds per panicle and seed set (SS; seeds/flower/panicle) were determined at harvest. Pollen viability was the variable most related to pollen germination and pollen tube growth. Stigma receptivity was not associated with its morphology. Growth of the pollen tube in stigma, style and ovary was observed in the transmitting tissue 18 HAP, running parallel to the vascular tissue. Yield under chilling field temperatures (minimum average of 6 and 8°C) prevailing during flower development and pollination ranged from 7 to 12 g/panicle. The differences in seed production and SS among A×B crosses did not depend on the amount and viability of pollen.

The inhibitory effect of CuSO4 on Citrus pollen germination and pollen tube growth and its application for the production of seedless fruit

Plant Science ◽  
2006 ◽  
Vol 170 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Carlos Mesejo ◽  
Amparo Martínez-Fuentes ◽  
Carmina Reig ◽  
Fernando Rivas ◽  
Manuel Agustí
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Inhibitory Effect ◽  
Tube Growth ◽  
Seedless Fruit

The Role of Boron, Silicon and Nucleic Bases on Pollen Tube Growth of Lilium longiflorum (L.)

1992 ◽  
Vol 47 (1-2) ◽  
pp. 102-108 ◽  
Author(s):  
J. Polster ◽  
M. Schwenk ◽  
E. Bengsch
Keyword(s):  
Pollen Tube ◽  
Boric Acid ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Sucrose Solution ◽  
Lilium Longiflorum ◽  
Tube Growth ◽  
Essential Trace Elements ◽  
Nucleic Bases

Abstract It is possible to obtain pollen germination and pollen tube growth in vitro if boric acid is present. In this work the effect was studied using as a semiquantitative parameter the mean length (l̄) of Lilium longiflorum pollen tubes. Pollen tube growth was examinated in dependence on boric acid, ortho-silicic acid, nucleic bases, Ca2+ and Zn2+ in 10% sucrose solution. The maximum of l̄ is obtained for concentrations between 2-20 ppm boron. The simultaneous supply of silicon added as water glass leads to a synergistic stimulation effect on pollen tube growth and facilitates branching. The silicon action is preceded of a pollen tube growth inhibition period during 3 h. Adenine and guanosine are able to substitute partially boron as pollen germination and pollen tube growth stimulator. Concentrations of 100 ppm adenine leads to half the boron effect. The same stimulation effect is obtained by guanosine. Ca2+ can partially substitute boron as well. The stimulation action of boron is significantly attenuated by Zn2+ and by the herbicide Dicuran. These and preceding results from physiological studies indicate that boron and silicon should be essential trace elements for the regulation of molecular biological processes.

scholarly journals Pollen Tube Growth in Carya and Temporal Influence of Pollen Deposition on Fertilization Success in Pecan

1992 ◽  
Vol 117 (2) ◽  
pp. 328-331 ◽  
Author(s):  
Robert D. Marquard
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Tubes ◽  
Fertilization Success ◽  
Tube Growth ◽  
In Vivo Studies ◽  
Pollen Deposition ◽  
Cape Fear

In vivo pollen tube growth of pecan [Carya illinoinensis (Wangenh.) K. Koch] was estimated to be ≈ 150 μm·hour-1 from 3 to 8 hours postpollination. Pollen tubes averaged 47, 194, 405, and 946 μm after 2, 3, 4, and 8 hours postpollination, respectively. Pollen tube growth was strongly influenced by temperature, and in vitro studies demonstrated pollen germination and tube growth were optimal at 27C for `Cape Fear' pecan. In in vivo studies, tubes of cross-pollen did not grow significantly faster than tubes of self-pollen. Pollen tubes of water hickory [C. aquatica (Michx. f.) Nutt.] grew significantly faster than those of C. illinoinensis. Bitternut [C. cordiformis (Wangenh.) K. Koch] and mockernut hickory (C. tomentosa Nutt.) pollen tubes grew significantly slower on pecan stigmas than did pecan pollen. Pollen arriving first on the stigma has a decided advantage for fertilization success of pecan. The fertilization success rate of pecan pollen arriving 24 hours after first pollen arrival was <3%.

Sign in / Sign up

Export Citation Format

Share Document