Pemanfaatan fenomena pembentukan buah partenokarpi dalam perspektif pertanian di Indonesia

Sari. Buah merupakan organ reproduktif yang memainkan peranan penting pada tanaman dalam proses perbanyakan tanaman dan penyebaran biji. Biji akan terbentuk akibat adanya proses polinasi dan fertilisasi. Pada beberapa jenis buah keberadaan biji tidak diinginkan, sehingga sekarang sudah banyak dikembangkan buah tanpa biji atau buah partenokarpi. Secara garis besar partenokarpi terdiri dari dua kelompok utama, yaitu partenokarpi alami dan partenokarpi buatan. Pisang, tomat, dan manggis merupakan contoh tanaman yang bersifat partenokarpi alami. Pembetukan buah partenokarpi dapat dilakukan melalui beberapa cara diantaranya adalah penggunaan hormone auksin/giberelin, iridiasi polen, perubahan jumlah kromosom, gene silencing, modifikasi gen, dan genome editing tools. Review ini menjelaskan mengenai pembentukan buah partenokarpi melalui pendekatan bioteknologi.Kata Kunci: Bioteknologi, partenokarpi alami, partenokarpi buatan, buah AbstractFruit is a reproductive organ that plays an important role in plants for plant propagation and seed dispersal. Seeds will be formed due to the process of pollination and fertilization. In some types of fruit, seed in the fruit is not expected, therfore recently many study have been conducted to develop seedless fruit (parthenocarpic fruit). Seedless fruit consists of two main groups namely natural and artificial partenocarpic. Bananas, tomatoes, and mangosteen are examples of natural parthenocarpy plants. Artificial parthenokarpi fruit can be developed in several methods including the use of plant hormone i.e. auksin or gibberellin, pollen iridiation, changes in chromosome number, gene silencing, gene modification, and genome editing tools. In this review, it is explained about the formation of parthenocarp fruit through a biotechnology approach. Keywords: Biotechnology, natural parthenocarpy, artificial parthenocarpy, and fruit

Seedless fruit pummelo induced by Gamma Ray irradiation: Fruit morphological characters and stability evaluation

Mariana BD, Arisah H, Yenni, Selvawajayanti M. 2018. Seedless fruit pummelo induced by Gamma Ray irradiation: Fruit morphological characters and stability evaluation. Biodiversitas 19: 656-661. Pummelo is one of native citrus crops found in South East Asia including Indonesia. It is well known due to its big size and distinct taste compared to other citrus fruits. However, it contains many seeds, which may reduce the market demand and customer preferences. Therefore, the objective of this study was to generate seedless citrus by mutation techniques using gamma-ray radiation. The mutant induction of Pamelo Nambangan using gamma-ray radiation was conducted in 2003 using bud wood as plant materials. The bud wood was then irradiated with the dosage of 20, 40, and 60 Gy. The buds then grafted to Japansche Citroen rootstocks and maintained properly until the fruits could be observed. After three years of selection based on performance and fruit evaluation on MV2 generation, a mutant plant derived from 20 Gy irradiation treatments were obtained with improved character on the number of seeds. Pamelo Nambangan has more than 40 seeds/fruit and the mutant has less than 10 seeds/fruit on average. In the seedless mutant, it was observed that the mutant has embryo sac abortion leading to seedless fruit and low pollen viability (7.7%). The mutant has been released in 2016 as a new seedless pummelo variety under the name of ‘Pamindo Agrihorti’.

Seed Production Enhanced by Antiauxin in the pat-2 Parthenocarpic Tomato Mutant

There is concern that high temperatures resulting from global warming could reduce fruit set of tomato (Solanum lycopersicum). However, fruit set of parthenocarpic tomato genotypes, which often bears seedless fruit, is not reduced when grown under a high temperature. The cause of seedless fruit development was studied with the aim of increasing the seed number in parthenocarpic tomato. Ovule number at anthesis in parthenocarpic and non-parthenocarpic fruit did not differ, but the proportion of undeveloped ovules increased with time after anthesis in parthenocarpic tomato, whereas most ovules in non-parthenocarpic tomato developed normally. Pollen grains germinated on the stigma and extruded pollen tubes in parthenocarpic and non-parthenocarpic tomatoes, but in parthenocarpic tomato, pollen tube elongation was markedly inhibited in the style base. Elongation of pollen tubes on agar containing indoleacetic acid (IAA) was depressed in parthenocarpic and non-parthenocarpic tomato plants. p-Chlorophenoxyisobutyric acid (PCIB), which inhibits auxin action, did not affect the fruit set and fresh weight in either type of tomato, although seed number per fruit in parthenocarpic tomato was significantly increased from 13 ± 2 to 74 ± 6 seeds by PCIB treatment. These results indicated that a high IAA concentration in the ovary of parthenocarpic tomato inhibited pollen tube elongation, and that poor fertilization resulted in failure of ovule development. Moreover, floral organs in parthenocarpic tomato were normally developed as in non-parthenocarpic tomato, and seed development could be induced in parthenocarpic tomato by PCIB treatment.