scholarly journals 1P-008 High-throughput, high-resolution cryoEM structural analysis of helical assemblies of biological macromolecules toward atomic resolution(Protein:Structure, The 47th Annual Meeting of the Biophysical Society of Japan)

2009 ◽  
Vol 49 (supplement) ◽  
pp. S64
Author(s):  
Takashi Fujii ◽  
Takayuki Kato ◽  
Keiichi Namba
Keyword(s):  
Structural Analysis ◽  
High Resolution ◽  
Annual Meeting ◽  
High Throughput ◽  
Atomic Resolution ◽  
Biological Macromolecules ◽  
Biophysical Society

scholarly journals Epoxidized graphene grid for high-throughput high-resolution cryoEM structural analysis

2021 ◽  
Author(s):  
Junso Fujita ◽  
Fumiaki Makino ◽  
Haruyasu Asahara ◽  
Maiko Moriguchi ◽  
Shota Kumano ◽  
...  
Keyword(s):  
Structural Analysis ◽  
High Resolution ◽  
Data Collection ◽  
High Throughput ◽  
Particle Density ◽  
Orientation Distribution ◽  
Biological Macromolecules ◽  
Density Maps ◽  
V1 Atpase ◽  
Density Map

Many specimens suffer from low particle density and/or preferred orientation in cryoEM specimen grid preparation, making data collection and structure determination time consuming. We developed an epoxidized graphene grid (EG-grid) that effectively immobilizes protein particles by applying an oxidation reaction using photoactivated ClO2· and further chemical modification. The particle density and orientation distribution are both dramatically improved, having enabled us to reconstruct the density map of GroEL and glyceraldehyde 3-phosphate dehydrogenase (GAPDH), at 1.99 and 2.16 Å resolution from only 504 and 241 micrographs, respectively. A low concentration sample solution of 0.1 mg ml–1 was sufficient to reconstruct a 3.10 Å resolution density map of SARS-CoV-2 spike protein from 1,163 micrographs. The density maps of V1-ATPase, β-galactosidase, and apoferritin were also reconstructed at 3.03, 1.81, and 1.29 Å resolution, respectively. These results indicate that the EG-grid will be a powerful tool for high-throughput cryoEM data collection to accelerate high-resolution structural analysis of biological macromolecules.

scholarly journals 1P302 A structural analysis of the MAVS-regulatory mechanism using BRET(27. Bioimaging,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

2014 ◽  
Vol 54 (supplement1-2) ◽  
pp. S191
Author(s):  
Osamu Sasaki ◽  
Takuma Yoshizumi ◽  
Misa Kuboyama ◽  
Takeshi Ishihara ◽  
Emiko Suzuki ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Annual Meeting ◽  
Regulatory Mechanism ◽  
Biophysical Society
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