Abstract
BackgroundCarbon tetrachloride (CCl4) is a widely used hepatic toxin that causes acute liver injury through pathological mechanisms such as oxidative stress, inflammation, and apoptosis. In this experiment, mice were treated with Lactobacillus plantarum (LP) HFY15, silymarin, and Lactobacillus delbruechii subsp. bulgaricus (LDSB) for two weeks, and intraperitoneal injection of CCl4-induced acute liver injury to study the preventive effect of LP-HFY on CCl4-induced acute liver injury, especially in oxidative damage. ResultsThe survival rate of LP-HFY15 in artificial gastric juice is 92.1%, and the growth efficiency in bile salt is 78.8%. Animal experiments show that LP-HFY15 reduces the liver index of mice, reduces the activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), the content of triglycerides (TG), malondialdehyde (MDA) and reactive oxygen species (ROS) in the serum of mice. LP-HFY15 also increased the antioxidant genes, such as nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase (HO-1), NAD(P)H:quinone oxidoreductase (NQO1), and increases the activity of superoxide dismutation (SOD), catalase (CAT), and glutathione (GSH) in the serum and gene level. At the same time, LP-HFY15 reduced the levels of cytokines IL-6, TNF-α, and IFN-γ in the serum and liver, increased antiapoptosis gene B-cell lymphoma-2 (Bcl-2) expression in the liver of mice, and inhibit the expression of proapoptotic genes Bcl-2-associated X (Bax) and Caspase-3. ConclusionsCompared with LDSB, LP-HFY15 has a greater alleviating effect on the liver injury caused by CCl4. These findings demonstrate that LP-HFY15 has great potential and research value in the future as a food supplement for preventing acute liver damage.
Preventive Effect of Flavonoid Extract from the Peel of Gonggan (Citrus reticulata Blanco Var. Gonggan) on CCl4-Induced Acute Liver Injury in Mice
