Scleral Lens and Prosthetic Replacement of the Ocular Surface Ecosystem Utilization in Ocular Graft-versus-Host Disease: A Survey Study

Abstract Background Diseases of the anterior segment of the eye may present different mechanisms, intensity of symptoms, and impact on the patients’ quality of life and vision. The tear film is in direct contact with the ocular surface and cornea and can be easily accessed for sample collection, figuring as a promising source of potential biomarkers for diagnosis and treatment control. This study aimed to evaluate tear proteomic profile in 3 distinct ocular diseases: keratoconus (corneal ectasia), severe dry eye related to graft-versus-host-disease (tear film dysfunction and ocular inflammatory condition) and pterygium (conjunctival fibrovascular degenerative disease). Methods Tear samples were collected from patients of each condition and a control group. By using mass spectrometric analysis combined with statistics and bioinformatics tools, a detailed comparison of protein profile was performed. Results After Student’s t-test analyses comparing each condition to the control group, we found the following number of differentially expressed proteins: 7 in keratoconus group, 29 in pterygium group, and 79 in GVHD group. Following multivariate analyses, we also report potential candidates as biomarkers for each disease. Conclusions We demonstrated herein that mass spectrometry-based proteomics was able to indicate proteins that differentiate three distinct ocular conditions, which is a promising tool for the diagnosis of ocular diseases.

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In Vivo Confocal Microscopy Evaluation of Ocular Surface with Graft-Versus-Host Disease-Related Dry Eye Disease

Scientific Reports ◽

10.1038/s41598-017-10237-w ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 18

Author(s):

Jingliang He ◽

Yoko Ogawa ◽

Shin Mukai ◽

Yumiko Saijo-Ban ◽

Mizuka Kamoi ◽

...

Keyword(s):

Confocal Microscopy ◽

Dry Eye ◽

Graft Versus Host Disease ◽

Ocular Surface ◽

Dry Eye Disease ◽

Eye Disease ◽

In Vivo Confocal Microscopy ◽

Host Disease ◽

Graft Versus Host

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Ocular surface indicators and biomarkers in chronic ocular graft-versus-host disease: a prospective cohort study

Bone Marrow Transplantation ◽

10.1038/s41409-021-01254-5 ◽

2021 ◽

Author(s):

Alexandra A. Pietraszkiewicz ◽

Debbie Payne ◽

Maria Abraham ◽

Angel Garced ◽

Krishna C. Devarasetty ◽

...

Keyword(s):

Cohort Study ◽

Graft Versus Host Disease ◽

Ocular Surface ◽

Cell Transplant ◽

Host Disease ◽

Graft Versus Host ◽

Schirmer’S Test ◽

Staining Score ◽

Tnf Α ◽

Corneal Staining

AbstractThis longitudinal cohort study compared ocular surface indicators in forty allogeneic hematopoietic stem cell transplant (HSCT) subjects with twenty healthy controls at baseline and identified changes in ocular graft-versus-host disease (oGVHD). Outcome measures included: Ocular Surface Disease Index (OSDI), tear osmolarity, Schirmer’s test, Oxford corneal staining score, tear break-up time (TBUT), and tear and serum biomarkers (IFN-γ, IL-10, MMP-9, IL-12, IL-13, IL-17α, IL-1β, IL-2, IL-4, IL-6, IL-8, CXCL10, MCP-1, MIP-1α, RANTES, TNF-α). At baseline the HSCT group had higher median Oxford corneal staining score (1.7 vs. 0.0; P < 0.0001), higher tear TNF-α (20.0 vs. 11.2 pg/mL; P < 0.0001), lower tear RANTES (70.4 vs. 190.2 pg/mL; P < 0.0001), higher serum IL-8 (10.2 vs. 4.5 pg/mL; P = 0.0008), and higher serum TNF-α (8.7 vs. 4.2 pg/mL; P < 0.0001). The incidence of oGVHD was 62% and associated changes included increased Oxford corneal staining score (4.6 vs. 1.8, P = 0.0001), decreased Schirmer’s test (3.0 vs. 10.0; P < 0.0001), and decreased TBUT (4.7 vs. 9.0 s; P = 0.0004). Baseline differences in ocular surface indicators suggest a tendency toward ocular dryness in individuals with hematologic disorders preparing for HSCT. Individuals who developed oGVHD showed changes in corneal staining score, Schirmer’s test, and TBUT.

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The disposable bandage soft contact lenses therapy and anterior segment optical coherence tomography for management of ocular graft-versus-host disease

BMC Ophthalmology ◽

10.1186/s12886-021-02031-0 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Yi-Chen Sun ◽

Yoshihiro Inamoto ◽

Ruikang K. Wang ◽

Stephanie J. Lee ◽

Kai-Feng Hung ◽

...

Keyword(s):

Optical Coherence Tomography ◽

Graft Versus Host Disease ◽

Ocular Surface ◽

Chronic Gvhd ◽

Anterior Segment ◽

Optical Coherence ◽

Soft Contact ◽

Host Disease ◽

Graft Versus Host ◽

Surface Changes

Abstract Purpose To identify the ocular surface changes of ocular graft-versus-host disease (GVHD) using anterior segment optical coherence tomography (AS-OCT) and examine the efficacy of disposable bandage soft contact lens (BSCL) treatment in ocular GVHD patients. Methods This study is a prospective, Phase II clinical trial. Nineteen patients diagnosed with chronic GVHD based on the NIH criteria and ocular symptoms of NIH eye score 2 or greater were enrolled. Disposable BSCL was applied to the GVHD-affected eyes with topical antibiotic coverage. Ocular exams, eye symptom surveys, and AS-OCT were performed with signed informed consent. Patients were followed for one to three months. Results Thirty-eight eyes of 19 patients with ocular GVHD underwent BSCL treatment in this study. AS-OCT scans were done in 14 out of 19 patients. The mean best-corrected visual acuity at enrollment, 2-week, and 4-week visits was 0.180, 0.128, and 0.163 logMAR, respectively. Twenty-four out of 25 eyes (96 %) that initially presented with conjunctival inflammation, twenty-three out of 30 eyes (76.7 %) that initially presented with punctate epithelial erosion, and 8 out of 15 (53.3 %) eyes that initially presented with filamentous keratopathy showed improvement after wearing BSCL for 2 to 4 weeks. AS-OCT revealed corneal epithelial irregularity, abnormal meibomian gland orifice, and conjunctival hyperemia, in patients with ocular GVHD. Conclusions BSCL treatment provided significant subjective and objective improvements in ocular GVHD patients. Meanwhile, we found that AS-OCT can be a promising diagnostic tool to characterize the ocular surface changes associated with ocular GVHD.

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