In vitro Acaricidal Activity of Ocimum gratissimum Essential Oil on Rhipicephalus sanguineus, Amblyomma sculptum and Rhipicephalus microplus Larvae

Baccharis dracunculifolia, native to Brazil and the main source of “green propolis”, has been reported with several biological activities, and may be a source of bovine tick control substituting synthetic acaricides. Objective: to evaluate the in vitro and ex situ acaricidal activity of B. dracunculifolia leaf and flower essential oils against Rhipicephalus microplus. Methodology: the essential oils were extracted by hydrodistillation and analyzed by a gas chromatography coupled to mass spectrometry; the acaricidal activity of the essential oil was evaluated in vitro against adult females and against the egg hatchability; moreover, the acaricidal activity against tick larvae was evaluated in vitro and ex situ. Results: the major class of the essential oils was oxygenated sesquiterpene (55.1% leaves 50.4% flowers) and the main compounds were (21.5% leaves; 20.6% flowers) and spathulenol (21.8% leaves; 20.3% flowers). The essential oil at 500 mg/mL was effective to control egg hatchability with a reduction of egg laying capacity and decrease of number of adult ticks and larvae. The larvicidal activity of the essential oil had LC99.9 from 35 to 37 mg/mL by probit analysis, and the essential oil from 11 to 14 mg/mL presented 85 to 95% of treatment efficiency in the ex situ test. Conclusion: B. dracunculifolia leaf and flower essential oils are stable and have application potential to control bovine ticks.

Download Full-text

Seasonal analysis and acaricidal activity of the thymol-type essential oil of Ocimum gratissimum and its major constituents against Rhipicephalus microplus (Acari: Ixodidae)

Parasitology Research ◽

10.1007/s00436-017-5662-0 ◽

2017 ◽

Vol 117 (1) ◽

pp. 59-65 ◽

Cited By ~ 11

Author(s):

Aldilene Silva Lima ◽

Marlise Neves Milhomem ◽

Odair Santos Monteiro ◽

Alana Carynne Pereira Arruda ◽

Joaquim Antônio Martins de Castro ◽

...

Keyword(s):

Essential Oil ◽

Rhipicephalus Microplus ◽

Acaricidal Activity ◽

Ocimum Gratissimum ◽

Seasonal Analysis

Download Full-text

In vitro evaluation of the acaricidal activity of Syzygium aromaticum (L.) essential oil and eugenol against non-fed larvae of Rhipicephalus sanguineus

Brazilian Journal of Veterinary Medicine ◽

10.29374/2527-2179.bjvm002620 ◽

2021 ◽

Vol 43 ◽

pp. e002620

Author(s):

Monique Moraes Lambert ◽

Douglas Siqueira de Almeida Chaves ◽

Barbara Rauta de Avelar ◽

Diefrey Ribeiro Campos ◽

Debora Azevedo Borges ◽

...

Keyword(s):

Essential Oil ◽

Rhipicephalus Sanguineus ◽

Acaricidal Activity ◽

Syzygium Aromaticum ◽

In Vitro Evaluation

Download Full-text

In vitro assessment of the acaricidal activity of carvacrol, thymol, eugenol and their acetylated derivatives on Rhipicephalus microplus (Acari: Ixodidae)

Veterinary Parasitology ◽

10.1016/j.vetpar.2018.07.009 ◽

2018 ◽

Vol 260 ◽

pp. 1-4 ◽

Cited By ~ 7

Author(s):

Tatiane Novato ◽

Geovany Amorim Gomes ◽

Viviane Zeringóta ◽

Cristiane Teixeira Franco ◽

Débora Ramos de Oliveira ◽

...

Keyword(s):

Rhipicephalus Microplus ◽

Acaricidal Activity ◽

In Vitro Assessment

Download Full-text

In Vitro Evaluation of Ethanolic Extracts ofAgeratum conyzoidesandArtemisia absinthiumagainst Cattle Tick,Rhipicephalus microplus

The Scientific World JOURNAL ◽

10.1155/2014/858973 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 8

Author(s):

S. Parveen ◽

R. Godara ◽

R. Katoch ◽

A. Yadav ◽

P. K. Verma ◽

...

Keyword(s):

Immersion Test ◽

Rhipicephalus Microplus ◽

Acaricidal Activity ◽

Cattle Tick ◽

Egg Mass ◽

Ageratum Conyzoides ◽

Artemisia Absinthium ◽

Aerial Parts ◽

Ethanolic Extracts

In vitro efficacy of ethanolic extracts obtained from the aerial parts ofAgeratum conyzoidesandArtemisia absinthiumwas assessed onRhipicephalus microplususing adult immersion test (AIT). Five concentrations of the extract (1.25%, 2.5%, 5%, 10%, and 20%) with three replications for each concentration were used in the bioassay. In AIT, the maximum mortality was recorded as 40% and 66.7% at 20% concentration forA. conyzoidesandA. absinthium, respectively. Acaricidal activity was found to be higher in the extract ofA. absinthiumwith LC50and LC95values of 11.2% and 61.7%, respectively. Egg mass weight of the live ticks treated with different concentrations of the extracts was significantly(P<0.05)lower than that of control ticks; consequently, the reproductive index and oviposition values of the treated ticks were reduced significantly(P<0.05). TheA. conyzoidesinhibited 90% hatching of eggs at the 20% concentration, whereasA. absinthiumshowed 100% inhibition at 5%, 10%, and 20% concentrations. The results show thatA. absinthiumhas better acaricidal properties thanA. conyzoidesand could be useful in controllingR. microplus.

Download Full-text

Efficacy of Tagetes minuta (Asteraceae) essential oil against Rhipicephalus sanguineus (Acari: Ixodidae) on infested dogs and in vitro

Experimental and Applied Acarology ◽

10.1007/s10493-016-0092-8 ◽

2016 ◽

Vol 70 (4) ◽

pp. 483-489 ◽

Cited By ~ 2

Author(s):

Elis Maressa Gonçalves da Silva ◽

Vinicius da Silva Rodrigues ◽

Jaciara de Oliveira Jorge ◽

Carolina Fonseca Osava ◽

Matias Pablo Juan Szabó ◽

...

Keyword(s):

Essential Oil ◽

Rhipicephalus Sanguineus ◽

Tagetes Minuta

Download Full-text

In vitro acaricidal activity of neem (Azadirachta indica) seed extracts with known azadirachtin concentrations against Rhipicephalus microplus

Veterinary Parasitology ◽

10.1016/j.vetpar.2011.03.053 ◽

2011 ◽

Vol 181 (2-4) ◽

pp. 309-315 ◽

Cited By ~ 17

Author(s):

R. Giglioti ◽

M.R. Forim ◽

H.N. Oliveira ◽

A.C.S. Chagas ◽

J. Ferrezini ◽

...

Keyword(s):

Azadirachta Indica ◽

Rhipicephalus Microplus ◽

Acaricidal Activity ◽

Seed Extracts

Download Full-text

Antifungal Activity of the Essential Oils from Ocimum gratissimum L. Grown in Togo

Journal of Scientific Research ◽

10.3329/jsr.v1i1.1131 ◽

2008 ◽

Vol 1 (1) ◽

pp. 164-171 ◽

Cited By ~ 11

Author(s):

Koffi Koba ◽

P W Poutouli ◽

Christine Raynaud ◽

Komla Sanda

Keyword(s):

Antifungal Activity ◽

Essential Oil ◽

Filamentous Fungi ◽

Essential Oil Composition ◽

Oil Composition ◽

Minimum Fungicidal Concentration ◽

Ocimum Gratissimum ◽

Aerial Parts ◽

In Vitro Antifungal Activity

The aerial parts of Ocimum gratissimum L. (Lamiaceae) harvested in Togo was steam-distilled and investigated for essential oil composition (GC and GC/MS) and in vitro antifungal activities. Thymol (31.79 %), p-cymene (15.57 %) and Î³-terpinene (12.34 %) and were the major components of the oil. Other notable components identified in this oil were myrcene (6.94 %) and Î±-thujene (6.11 %).The in vitro antifungal activity was recorded with the minimum inhibitory concentrations (MICs) ranging from 80 to 150Â Âµl.l-1, 150 to 500 Âµl.l-1 Â and from 100 to 150 Âµl.l-1 respectively on dermatophytes, imperfect filamentous fungi and pathogenic yeasts. Likewise, on tested fungi the minimum fungicidal concentration (MFC) varied from 300 Âµl.l-1 to 500 Âµl.l-1, 500 to 700 Âµl.l-1 and from 250 to 300 Âµl.l-1, respectively on dermatophytes, imperfect filamentous fungi and pathogenic yeasts. Keywords: O.gratissimum,Â Antifungal, Essential oil; Thymol.Â Â© 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.Â DOI: 10.3329/jsr.v1i1.1131Â

Download Full-text