Investigation on the effects of copper deficiency on fertility and some hematobiochemical parameters in rams with trial for treatment

Copper deficiency can cause cardiovascular lesions in experimental animals. Previous experiments have shown that the biochemical and itDrphologic lesions induced by deprivation of dietary copper can be suppressed by feeding diets containing starch or can be magnified by a high sucrose diet. In a recent study it was found that the more severe signs of copper deficiency in rats fed sucrose as compared to starch were due to the fructose moiety of sucrose. Although fructose as compared to starch markedly enhanced the symptoms of copper deficiency, the possibility that an effect of dietary carbohydrates due to the nature of the simple carbohydrate (fructose vs glucose) cannot be excluded. The present study was designed to determine if the severity of copper deficiency in rats fed sucrose as compared to starch is due to the glucose as well as the fructose moiety of sucrose. This portion of the study assessed the morphologic changes in aortas of seventy weanling male rats who were fed, for 9 weeks, copper deficient or copper supplemented diets containing either 62% starch, fructose or glucose. The starch-fed copper supplemented group served as the most normal controls. Rats were sacrificed after 9 weeks of dietary treatments. Copper deficiency was verified by reduced serum ceruloplasmin activity and serum and hepatic copper concentration.

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Abstract #337: A Rare Case of Copper Deficiency and Celiac Disease

Endocrine Practice ◽

10.1016/s1530-891x(20)44083-2 ◽

2006 ◽

Vol 12 ◽

pp. 96-97

Author(s):

Mihaela Cosma ◽

Daniel L. Hurley

Keyword(s):

Celiac Disease ◽

Rare Case ◽

Copper Deficiency

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Acute and Sub-acute Oral Toxicity Assessment of a Standardized Polyherbal Preparation POL-6 in Rats

The Natural Products Journal ◽

10.2174/2210315508666181004100520 ◽

2019 ◽

Vol 9 (3) ◽

pp. 207-216

Author(s):

Lalit Sharma ◽

Aditi Sharma ◽

Girdhari L. Gupta ◽

Gopal Singh Bisht

Keyword(s):

Histopathological Examination ◽

Safety Evaluation ◽

Centella Asiatica ◽

Toxicity Assessment ◽

Ocimum Sanctum ◽

Oral Toxicity ◽

Significant Treatment ◽

Body Weights ◽

Total Body ◽

Hematobiochemical Parameters

Background: A standardized polyherbal preparation (POL-6) containing six plant extracts Hypericum perforatum, Bacopa monnieri, Centella asiatica, Withania somnifera, Ocimum sanctum and Camellia sinesis have good antioxidant, anti-inflammatory, and immunomodulatory activities. The present study was carried out to evaluate the safety profile of POL-6 through acute and subacute oral toxicity models in Wistar rats. Methods: In acute safety evaluation, a single dose of 2000mg/kg of POL-6 was given orally to five rats and was observed for 14 days. In subacute safety evaluation POL-6 at the doses of 250, 500 and 1000 mg/kg was given orally to the rats once a day for 28 days. The animals were observed for the signs of toxicity and mortality during the study period. Results: In acute toxicity evaluation, POL-6 treatment did not show any toxic signs and mortality in animals during the observation period. In subacute toxicity studies, no changes were seen in any of the dose levels of POL-6 treatment during the total body weights, organ weights and hematobiochemical parameters examination of the rats. No lesions were seen during the gross/histopathological examination. Conclusion: The study revealed that administration of POL-6 for 28 days showed no significant treatment generated toxic effects in the animals, hence it can be considered as non-toxic if it is ingested in a time not greater than a month.

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Pancytopenia in a child with cystic fibrosis and severe copper deficiency: Insight from bone marrow evaluation

Pediatric Blood & Cancer ◽

10.1002/pbc.29276 ◽

2021 ◽

Author(s):

Maggie D. Seblani ◽

Susanna A. McColley ◽

Shunyou Gong ◽

Lee M. Bass ◽

Sherif M. Badawy

Keyword(s):

Cystic Fibrosis ◽

Bone Marrow ◽

Copper Deficiency ◽

Bone Marrow Evaluation

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Effect of Leukoreduction on Hematobiochemical Parameters and Storage Hemolysis in Canine Whole Blood Units

Animals ◽

10.3390/ani11040925 ◽

2021 ◽

Vol 11 (4) ◽

pp. 925

Author(s):

Maria Teresa Antognoni ◽

Maria Luisa Marenzoni ◽

Ambra Lisa Misia ◽

Luca Avellini ◽

Elisabetta Chiaradia ◽

...

Keyword(s):

Whole Blood ◽

Complete Blood Count ◽

Membrane Resistance ◽

Progressive Increase ◽

Rbc Membrane ◽

Morphological Index ◽

The Difference ◽

Hematobiochemical Parameters ◽

And Storage ◽

Individual Donor

Storage lesions (SLs) occur when the red blood cell quality is altered during the preservation of blood units. Pre-storage leukoreduction would limit the number of SLs. The aims of this study were to evaluate the effectiveness of a leukoreduction filter for human use and the effect of pre-storage leukoreduction on some ematobiochemical parameters in stored canine whole blood. Seven canine blood units were tested. Each one was divided into two units—one leukoreduced (LRWB) and one non-leukoreduced (nLRWB). On each unit, we determined the complete blood count (CBC), lactate-dehydrogenase (LDH), electrolytes (Na+, K+, Cl−), morphological index (MI) and hemolysis, on storage days 0, 7, 14, 21, 28, 35, and 42. Leukoreduction allowed a 98.30% recovery of the RBC count, retaining 99.69% and 94.91% of WBCs and PLTs, respectively. We detected a significant increase of LDH and MI with strongly higher values in nLRWB compared to LRWB. A progressive increase in electrolytes and LDH concentrations was observed as indices of stored hemolysis. LDH showed significantly lower values in LRWB units compared to nLRWB, suggesting its release from leukocytes. In the majority of units, hemolysis reached 1% on the 42nd day of storage. We assert the human leukoreduction filter effectiveness on canine whole blood, and we recommend using nLRWB before day 14, especially for critically ill patients. The difference of the basal hemolysis (day 0) percentages observed between subjects suggests that more studies should be performed to confirm a possible inter-individual donor biological variability of RBC membrane resistance, as happens in humans.

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The Occurrence of Conditioned and Simple Copper Deficiency in Cattle and Sheep in Setesdalen, a Valley in the Southern Part of Norway II

Acta Veterinaria Scandinavica ◽

10.1186/bf03547358 ◽

1961 ◽

Vol 2 (1) ◽

pp. 375-398

Author(s):

Gustav N. Havre ◽

Olav Dynna

Keyword(s):

Copper Deficiency ◽

Cattle And Sheep

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A case of Menkes syndrome with pyloric stenosis: An effect of copper deficiency on NOS1?

Journal of Inherited Metabolic Disease ◽

10.1023/a:1005487027838 ◽

1999 ◽

Vol 22 (2) ◽

pp. 197-198 ◽

Cited By ~ 2

Author(s):

S. Cunningham ◽

P. M. Crofton ◽

T. G. Marshall ◽

D. R. Fitzpatrick

Keyword(s):

Pyloric Stenosis ◽

Copper Deficiency ◽

Effect Of Copper

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Erythropoietin-Resistant Anemia Secondary to Zinc-Induced Hypocupremia in a Hemodialysis Patient

Case Reports in Nephrology and Dialysis ◽

10.1159/000512612 ◽

2021 ◽

pp. 167-175

Author(s):

Stephanie Munie ◽

Pairach Pintavorn

Keyword(s):

Copper Deficiency ◽

Hemodialysis Patient ◽

Esrd Patient ◽

Serological Testing ◽

Zinc Supplement ◽

Excessive Intake ◽

Stage Renal Disease ◽

End Stage ◽

Work Up ◽

Excess Zinc

Excessive intake of zinc is a known but often forgotten cause of copper deficiency, and its consequences in the context of end-stage renal disease (ESRD) are not widely discussed. Zinc-induced copper deficiency (ZICD) can result in erythropoietin (EPO)-resistant anemia and may not be considered as a possible etiology when conducting the work-up. We present a case wherein an ESRD patient had been receiving excess zinc for several months and subsequently experienced EPO-resistant anemia. Our patient’s GI work-up was negative, and increased doses of iron and EPO-stimulating agent were ineffective. She underwent a bone marrow biopsy and more serological testing. She was ultimately diagnosed with ZICD, and cessation of her zinc supplement and initiation of copper replacement proved effective in restoring EPO responsiveness. Awareness of ZICD as a possible factor in EPO-resistant anemia could lead to an expedited diagnosis and avoid an unnecessary and extensive work-up.

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