GENETIC DIVERSITY AND RELATIONSHIPS IN Chrysanthemum morifolium AS REVEALED BY RAPD MOLECULAR MAKER, AND SOME MORPHOLOGICAL CHARACTERS

<p>Indonesia is rich in rice genetic resources, however, only a small number has been used in variety improvement programs. This study aimed to determine the genetic diversity of Indonesian rice varieties using 6K SNP markers. The study was conducted at ICABIOGRAD for DNA isolation and IRRI for SNP marker analysis. Genetic materials were 53 rice genotypes consisting of 49 varieties and 4 check genotypes. SNP markers used were 6K loci. Results showed that among the markers analyzed, only 4,606 SNPs (76.77%) were successfully read. The SNP markers covered all twelve rice chromosomes of 945,178.27 bp. The most common allele observed was GG, whereas the least allele was TG. Dendrograms of the 53 rice varieties analyzed with 4,606 SNPs demonstrated several small groups containing genotypic mixtures between indica and japonica rice, and no groups were found to contain firmly indica or japonica type. Structure analysis (K = 2) with value of 0.8 showed that the 53 rice varieties were divided into several groups and each group consisted of 4 japonica, 2 tropical japonica, 46 indica, and 1 aus rice type, respectively. IR64 and Ciherang proved to have an indica genome, while Rojolele has japonica one. Dupa and Hawara Bunar, usually grouped into tropical japonica rice, were classified as indica type, and Hawara Bunar has perfectly 100% indica type. The results of this study indicated that rice classification (indica-japonica) which is usually classified based only on morphological characters, e.g. grain and leaf shapes, is not enough and classification based on SNP markers should be considered for that purpose.</p>

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Morphological and genetic diversity of Senecio vulgaris L. (Asteraceae) in Iran

Acta Botanica Croatica ◽

10.37427/botcro-2021-012 ◽

2021 ◽

Vol 80 (2) ◽

Author(s):

Mostafa Ebadi ◽

Rosa Eftekharian

Keyword(s):

Genetic Diversity ◽

Principal Component ◽

Morphological Characters ◽

Mantel Test ◽

Morphological Data ◽

Principal Coordinates Analysis ◽

Cauline Leaf ◽

Senecio Vulgaris ◽

Molecular Features ◽

Principal Coordinates

Senecio vulgaris L., an annual herb belonging to the Asteraceae, is widely distributed in different regions of the world. There is no information on the intraspecific variations of the morphological and molecular features of this species. In the present investigation, we studied the morphological and genetic diversity of 81 accessions of S. vulgaris collected from 10 geographical populations. Eleven inter simple sequence repeat (ISSR) primers were used for the examination of genetic variations among the populations. Analysis of molecular variance (AMOVA) and GST analyses revealed significant differences among the investigated populations. A significant correlation between genetic distance and geographical distance was revealed by the Mantel test. However, reticulation analysis indicated the occurrence of gene flow among most of the populations studied. Principal component analysis (PCA) plot showed that the number of capitula, length of the cauline leaf and plant height were the most variable morphological characters. Principal coordinates analysis (PCoA) plot revealed two groups of populations, according to molecular and morphological data. The results suggested the existence of possible intraspecific taxonomic ranks within this species.

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Diversity of capsaicin content, quantitative, and yield components in chili (Capsicum annuum) genotypes and their F1 hybrid

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d210555 ◽

2020 ◽

Vol 21 (5) ◽

Author(s):

Zulfikar D Sahid ◽

MUHAMAD SYUKUR ◽

AWANG MAHARIJAYA

Keyword(s):

Genetic Diversity ◽

Capsicum Annuum ◽

Block Design ◽

Genetic Material ◽

Morphological Characters ◽

Fruit Weight ◽

Yield Component ◽

Quantitative Yield ◽

F1 Hybrid ◽

Randomized Complete Block Design

Abstract. Sahid ZD, Syukur M, Maharijaya A. 2020. Genetic diversity of capsaicin content, quantitative, and yield component in chili (Capsicum annuum) and their F1 hybrid. Biodiversitas 21: 2251-2257. Chili (Capsicum annuum L.) is one of the horticultural plants that have many benefits. The benefit of chili was determined by pungency level of its fruit. Pungency level of the chili is due to the capsaicin content in fruit. Information about the genetic diversity of capsaicin is still rarely available. The aims of this study were to obtain diversity information on quantitative, yield component, and capsaicin content, and to analyze the correlation among chili genotypes based on their morphological characters. This study used Randomized Complete Block Design with three replications. The genetic material used in this study consisted of 21 genotypes consisting of 6 genotypes of chili elders and 15 hybrid F1 genotypes resulting from their crossing. Six genotypes of the chili parents are C5, F6074, F9160291, Yuni, Bara, and Giant. 15 hybrid F1 genotypes used in this study are C5 x Bara, C5 x F6074, C5 x Yuni, C5 x Giant, C5 x F9160291, Bara x F6074, Bara x Yuni, Bara x Giant, Bara x F9160291, F6074 x Yuni, F6074 x Giant, F6074 x F9160291, Yuni x Giant, Yuni x F9160291, and Giant x F9160291. The observation was made on the variables of quantitative, yield, and capsaicin components on chili. The results showed that the highest capsaicin content only was found in Bara x F9160291. The results of scatterplot analysis showed that the highest capsaicin and yield component was found in BaraxF6074 and C5 x Yuni genotype. The results of cluster analysis showed that chili was clustered into three color groups. The character of capsaicin content is negatively correlated and very different from fruit weight, fruit diameter, fruit length, thick fruit flesh, total amount of fruit per plant, and fruit weight per plant.

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Genetic diversity of Echinacea species based upon amplified fragment length polymorphism markers

Genome ◽

10.1139/g03-086 ◽

2004 ◽

Vol 47 (1) ◽

pp. 102-111 ◽

Cited By ~ 17

Author(s):

D -H Kim ◽

D Heber ◽

D W Still

Keyword(s):

Genetic Diversity ◽

Genetic Distance ◽

Fragment Length ◽

Echinacea Purpurea ◽

Morphological Characters ◽

Pairwise Distance ◽

Neighbor Joining ◽

Aflp Data ◽

Phenetic Relationships ◽

Length Polymorphisms

The taxonomy of Echinacea is based on morphological characters and has varied depending on the monographer. The genus consists of either nine species and four varieties or four species and eight varieties. We have used amplified fragment length polymorphisms (AFLP) to assess genetic diversity and phenetic relationships among nine species and three varieties of Echinacea (sensu McGregor). A total of 1086 fragments, of which approximately 90% were polymorphic among Echinacea taxa, were generated from six primer combinations. Nei and Li's genetic distance coefficient and the neighbor-joining algorithm were employed to construct a phenetic tree. Genetic distance results indicate that all Echinacea species are closely related, and the average pairwise distance between populations was approximately three times the intrapopulation distances. The topology of the neighbor-joining tree strongly supports two major clades, one containing Echinacea purpurea, Echinacea sanguinea, and Echinacea simulata and the other containing the remainder of the Echinacea taxa (sensu McGregor). The species composition within the clades differs between our AFLP data and the morphometric treatment offered by Binns and colleagues. We also discuss the suitability of AFLP in determining phylogenetic relationships.Key words: Echinacea, AFLP, genetic distance, phylogeny.

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Genetic diversity and relationships of medicinal Chrysanthemum morifolium revealed by start codon targeted (SCoT) markers

Scientia Horticulturae ◽

10.1016/j.scienta.2016.01.042 ◽

2016 ◽

Vol 201 ◽

pp. 118-123 ◽

Cited By ~ 10

Author(s):

Shang-Guo Feng ◽

Ren-Feng He ◽

Meng-Ying Jiang ◽

Jiang-Jie Lu ◽

Xiao-Xia Shen ◽

...

Keyword(s):

Genetic Diversity ◽

Chrysanthemum Morifolium ◽

Start Codon

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Evaluation of Genetic Diversity and Interrelationships of Agro-Morphological Characters in Soybean (Glycine max) Genotypes

Proceedings of the National Academy of Sciences India Section B Biological Sciences ◽

10.1007/s40011-014-0356-1 ◽

2014 ◽

Vol 85 (2) ◽

pp. 397-405

Author(s):

Amit Kumar ◽

Avinash Pandey ◽

Chubasenla Aochen ◽

A. Pattanayak

Keyword(s):

Genetic Diversity ◽

Glycine Max ◽

Morphological Characters

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Indications of Genetic Admixture in the Transition Zone between Fagus sylvatica L. and Fagus sylvatica ssp. orientalis Greut. & Burd

Diversity ◽

10.3390/d11060090 ◽

2019 ◽

Vol 11 (6) ◽

pp. 90 ◽

Cited By ~ 2

Author(s):

Markus Müller ◽

Precious Annie Lopez ◽

Aristotelis C. Papageorgiou ◽

Ioannis Tsiripidis ◽

Oliver Gailing

Keyword(s):

Genetic Diversity ◽

Fagus Sylvatica ◽

Expressed Sequence Tag ◽

European Beech ◽

Morphological Characters ◽

Genetic Admixture ◽

Southeast Europe ◽

Fagus Orientalis Lipsky ◽

Specific Allele ◽

Fagus Sylvatica L

Two subspecies of European beech (Fagus sylvatica L.) can be found in southeast Europe: Fagus sylvatica ssp. sylvatica L. and Fagus sylvatica ssp. orientalis (Lipsky) Greut. & Burd. (Fagus orientalis Lipsky). In a previous study, based on genetic diversity patterns and morphological characters, indications of hybridization between both subspecies were found in northeastern Greece, a known contact zone of F. sylvatica and F. orientalis. Nevertheless, potential genetic admixture has not been investigated systematically before. Here, we investigated genetic diversity and genetic structure of 14 beech populations originating from Greece and Turkey as well as of two reference F. sylvatica populations from Germany based on nine expressed sequence tag-simple sequence repeat (EST-SSR) markers. Very low genetic differentiation was detected among F. sylvatica populations (mean GST: 0.005) as well as among F. orientalis populations (mean GST: 0.008), but substantial differentiation was detected between populations of the two subspecies (mean GST: 0.122). Indications for hybridization between both subspecies were revealed for one population in Greece. One of the genetic markers showed specific allele frequencies for F. sylvatica and F. orientalis and may be used as a diagnostic marker in future studies to discriminate both subspecies.

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