GENETIC VARIABILITY STUDY OF Phytophthora infestans ISOLATES BASED ON MATING TYPE MOLECULAR MARKERS AND METALAXYL RESISTANCE

2012 ◽  
Vol 3 (3) ◽  
pp. 227-238
Author(s):  
Mona Ragab ◽  
M. Mostafa ◽  
S. Abdel-Momen ◽  
Marwa Ismail
Keyword(s):  
Molecular Markers ◽  
Genetic Variability ◽  
Phytophthora Infestans ◽  
Mating Type ◽  
Metalaxyl Resistance ◽  
Variability Study

The structure of mating type, virulence, metalaxyl resistance of Phytophthora infestans in a long-term phenotypic study in distinct location in Eastern Estonia

2012 ◽  
Vol 119 (2) ◽  
pp. 45-52 ◽  
Author(s):  
Eve Runno-Paurson ◽  
Asko Hannukkala ◽  
Stanislav Trdan ◽  
Ingrid Williams ◽  
Mati Koppel ◽  
...  
Keyword(s):  
Phytophthora Infestans ◽  
Mating Type ◽  
Metalaxyl Resistance

Metalaxyl resistance, mating type and pathogenicity of Phytophthora infestans in Uganda

2001 ◽  
Vol 20 (5) ◽  
pp. 379-388 ◽  
Author(s):  
J. Mukalazi ◽  
E. Adipala ◽  
T. Sengooba ◽  
J.J. Hakiza ◽  
M. Olanya ◽  
...  
Keyword(s):  
Phytophthora Infestans ◽  
Mating Type ◽  
Metalaxyl Resistance

scholarly journals Characterization of Isolates of Phytophthora infestans from Southern and Southeastern Brazil from 1998 to 2000

Plant Disease ◽  
2003 ◽  
Vol 87 (8) ◽  
pp. 896-900 ◽  
Author(s):  
Ailton Reis ◽  
Christine D. Smart ◽  
William E. Fry ◽  
Luiz A. Maffia ◽  
Eduardo S. G. Mizubuti
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Phytophthora Infestans ◽  
Mating Type ◽  
Fragment Length Polymorphism ◽  
Southeastern Brazil ◽  
Mating Types ◽  
Clonal Lineage ◽  
Metalaxyl Resistance ◽  
Restriction Fragment Length

The population of Phytophthora infestans in Brazil was first characterized 12 years ago. In this research, isolates of P. infestans from potato (n = 184) and tomato (n = 267) collected in southern and southeastern Brazil were characterized to provide more detailed analysis of the current structure of the population. All 451 isolates were analyzed for mating type, and subsets of the isolates were analyzed for allozymes, restriction fragment length polymorphism fingerprint, mtDNA haplotypes, and metalaxyl resistance. Tomato isolates were all of A1 mating type, mtDNA Ib, and US-1 genotype or some variant within this clonal lineage. Of the potato isolates, 82% were A2 mating type, mtDNA IIa, BR-1 genotype, which is a new lineage of P. infestans. All A2 isolates were found on potato, whereas 91% of the A1 isolates were from tomato. A1 and A2 isolates were never found in the same field. The frequency of resistance to metalaxyl was higher in isolates from tomato (55%) than in isolates from potato (38%). After more than a decade of coexistence of isolates of the A1 and A2 mating types, the population was highly clonal, dominated by the BR-1 and US-1 clonal lineages.

scholarly journals Assessment of genetic variation in Linum L. using SPAR markers

Biologija ◽  
2017 ◽  
Vol 63 (1) ◽  
Author(s):  
Zahra Noormohammadi ◽  
Mina Sakhaee ◽  
Masoud Sheidai ◽  
Seyed Mehdi Talebi
Keyword(s):  
Molecular Markers ◽  
Genetic Variability ◽  
Linum Usitatissimum ◽  
Genetic Erosion ◽  
Network Analyses ◽  
Low Degree ◽  
Breeding Programmes ◽  
Variability Study ◽  
Single Primer ◽  
Amplification Reaction

Flax (Linum usitatissimum subsp. usitatissimum) is an important crop plant cultivated both for its fiber and seed oil content. Uniform cultivation and selection practice may lead to genetic erosion and reduced genetic variability of Linum cultivars, which in turn makes the crop vulnerable to diseases and pathogens. Therefore we performed a genetic diversity analysis of few populations including both Linum usitatissimum subsp. usitatissimum L. and L. Bienne L. We used a combination of single primer amplification reaction markers, including RAPD, ISSR, and RAMPO molecular markers for genetic variability study. In general, all three molecular markers revealed a  low degree of genetic variability in the  materials studied; however, L. bienne had a  higher level of genetic variability. Clustering and network analyses grouped the studied plants inter-mixed and did not separate the studied species or populations. STRUCTURE plot and K-Means clustering also identified these populations as genetically homogenous. These results indicate the need for introducing new linseed cultivars into the country for future hybridization and breeding programmes.

scholarly journals The structure of mating type, metalaxyl resistance and virulence of Phytophthora infestans isolates collected from Latvia

2015 ◽  
Vol 102 (3) ◽  
pp. 335-342 ◽  
Author(s):  
Alice Aav ◽  
Ilze Skrabule ◽  
Gunita Bimšteine ◽  
Tanel Kaart ◽  
Ingrid H. Williams ◽  
...  
Keyword(s):  
Phytophthora Infestans ◽  
Mating Type ◽  
Metalaxyl Resistance

Mating type, Metalaxyl Resistance and Aggressiveness of Phytophthora infestans (Mont.) de Bary in Morocco

2002 ◽  
Vol 150 (4-5) ◽  
pp. 289-291 ◽  
Author(s):  
A. HAMMI ◽  
Y. MSATEF ◽  
A. BENNANI ◽  
A. E. L. ISMAILI ◽  
M. N. SERRHINI
Keyword(s):  
Phytophthora Infestans ◽  
Mating Type ◽  
Metalaxyl Resistance

Mating Type, Virulence, Aggressiveness and Metalaxyl Resistance of Isolates of Phytophthora Infestans in Poland

2007 ◽  
Vol 49 (3) ◽  
pp. 155-166 ◽  
Author(s):  
Jadwiga Śliwka ◽  
Sylwester Sobkowiak ◽  
Renata Lebecka ◽  
Jose Avendaño-Córcoles ◽  
Ewa Zimnoch-Guzowska
Keyword(s):  
Phytophthora Infestans ◽  
Mating Type ◽  
Metalaxyl Resistance

scholarly journals First Report from Morocco of Phytophthora infestans Isolates with Metalaxyl Resistance

Plant Disease ◽  
1997 ◽  
Vol 81 (7) ◽  
pp. 831-831 ◽  
Author(s):  
M. Sedegui ◽  
R. B. Carroll ◽  
A. L. Morehart ◽  
A. Arifi ◽  
R. Lakhdar
Keyword(s):  
Phytophthora Infestans ◽  
Mating Type ◽  
Solanum Tuberosum L ◽  
Relative Sensitivity ◽  
Control Programs ◽  
Growing Seasons ◽  
Metalaxyl Resistance ◽  
Commercial Potato ◽  
Tomato Cultivars ◽  
Allozyme Loci

Late blight of potato (Solanum tuberosum L.) caused by Phytophthora infestans (Mont.) de Bary first appeared in Africa in 1941. It has been observed sporadically in Morocco for decades but only recently became a major problem. Significant losses have been recorded in the last two growing seasons in spite of the use of various disease control programs that included combinations of systemic and protectant fungicides. Phytophthora infestans was cultured from diseased foliage collected from commercial potato fields near Larache, Morocco. Isolates were analyzed to determine pathogenicity on several potato and tomato cultivars, mating type, genotype at two allozyme loci (2), and relative sensitivity to metalaxyl. Responses of the isolates to metalaxyl were assayed by mycelial radial growth on metalaxyl-amended agar, by floating leaves inoculated with P. infestans on metalaxyl solutions, and via potato tuber disks placed on filter paper saturated with metalaxyl solutions (1). Koch's postulates were completed; all isolates were pathogenic to potato and tomato cultivars tested, are consistent with the A1 mating type, and have the same allozyme pattern (Gpi 100/100, Pep 92/100) as US-6 genotype. All tests indicated resistance to metalaxyl up to 250 ppm. References: (1) K. L. Deahl et al. Am. Potato J. 70:779, 1993. (2) S. B. Goodwin et al. Plant Dis. 79:1181, 1995.

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