scholarly journals Assessment of genetic variation in Linum L. using SPAR markers

Biologija ◽  
2017 ◽  
Vol 63 (1) ◽  
Author(s):  
Zahra Noormohammadi ◽  
Mina Sakhaee ◽  
Masoud Sheidai ◽  
Seyed Mehdi Talebi
Keyword(s):  
Molecular Markers ◽  
Genetic Variability ◽  
Linum Usitatissimum ◽  
Genetic Erosion ◽  
Network Analyses ◽  
Low Degree ◽  
Breeding Programmes ◽  
Variability Study ◽  
Single Primer ◽  
Amplification Reaction

Flax (Linum usitatissimum subsp. usitatissimum) is an important crop plant cultivated both for its fiber and seed oil content. Uniform cultivation and selection practice may lead to genetic erosion and reduced genetic variability of Linum cultivars, which in turn makes the crop vulnerable to diseases and pathogens. Therefore we performed a genetic diversity analysis of few populations including both Linum usitatissimum subsp. usitatissimum L. and L. Bienne L. We used a combination of single primer amplification reaction markers, including RAPD, ISSR, and RAMPO molecular markers for genetic variability study. In general, all three molecular markers revealed a  low degree of genetic variability in the  materials studied; however, L. bienne had a  higher level of genetic variability. Clustering and network analyses grouped the studied plants inter-mixed and did not separate the studied species or populations. STRUCTURE plot and K-Means clustering also identified these populations as genetically homogenous. These results indicate the need for introducing new linseed cultivars into the country for future hybridization and breeding programmes.

scholarly journals Genetic finger printing of cotton cultivars by ISSR molecular markers

Genetika ◽  
2018 ◽  
Vol 50 (2) ◽  
pp. 627-634 ◽  
Author(s):  
Farah Farahani ◽  
Masoud Sheidai ◽  
Fahimeh Koohdar
Keyword(s):  
Molecular Markers ◽  
Genetic Variability ◽  
Gossypium Hirsutum ◽  
Agronomic Traits ◽  
Genetic Erosion ◽  
Continuous Selection ◽  
Tetraploid Cotton ◽  
Genetic Affinity ◽  
Cotton Species ◽  
Issr Molecular Markers

Gossypium hirsutum is one of the main tetraploid cotton species that is cultivated throughout the world. Due to continuous selection of cotton cultivars for specific agronomic traits, the genetic variability within the cultivars decrease that lead to genetic erosion. To tackle the problem of reduced genetic variability, we should track all available genetic diversity within cotton germplasm and use them for inter-specific and intra-specific hybridization and produce new elite cotton cultivars. Therefore, the present study used ISSR molecular markers to illustrate genetic variability in 13 tetraploid cotton genotypes (Gossypium hirsutum L.) and to categorize these genotypes based on genetic affinity. 65 cotton plants were studied. The results identified private bands in the studied genotypes, while Network and STRUCTURE analyses of molecular data obtained grouped the genotypes with genetic affinity together. Some of the genotypes differed in their genetic content from the others; therefore, studying the genetic and agronomic variability within available cultivars is very important and produced data to broaden the gene pool for planning further hybridization in cotton.

PROFILING OF GUAVA GENOTYPES GROWN IN VIDARBHA CONDITIONS ON THE BASIS OF MORPHOLOGICAL TRAITS

2017 ◽  
Vol 23 (1) ◽  
Author(s):  
R.A. PATIL ◽  
S.G. BHARAD ◽  
S.N. SAWANT
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Morphological Traits ◽  
Considerable Extent ◽  
Fruit Traits ◽  
Breeding Programmes ◽  
Tree Leaf ◽  
Qualitative Characteristics ◽  
Intrapopulation Diversity

Assessment of genetic diversity in the available germplasm is the prerequisite for development of improved genotypes through planned breeding programmes. In the view of this Forty-eight genotypes of seedling origin guava along with 1 check (L-49/Sardar) collected and conserved at germplasm block, Main Garden, Department of Horticulture, Dr. P. D. A. University, Akola were evaluated for genetic variability and diversity based on the qualitative characteristics. The genotypes were evaluated for sixteen morphological traitsviz. tree, leaf, floral and fruit traits. Results Show considerable extent of variability amongst the 49 genotypes in each traits. A sizeable amount of intrapopulation diversity recorded can be used to identify diverse parents which can be utilized in hybridization programmes.

Genetic variability study of elite guar (Cyamopsis tetragonoloba L.) germplasm as revealed by SDS-page method

2018 ◽  
Vol 51 (2) ◽  
Author(s):  
Sohail Ahmad Jan ◽  
Zabta Khan Shinwari ◽  
Malik Ashiq Rabbani ◽  
Ali Talha Khalil ◽  
Azhar Hussain Shah
Keyword(s):  
Genetic Variability ◽  
Cyamopsis Tetragonoloba ◽  
Sds Page ◽  
Variability Study

Genetic Variability and Geographic Typicality of Italian Former Prosecco Grape Variety Using PCR-Derived Molecular Markers

2013 ◽  
Vol 56 (5) ◽  
pp. 408-420 ◽  
Author(s):  
Stefano Meneghetti ◽  
Angelo Costacurta ◽  
Luigi Bavaresco ◽  
Antonio Calo’
Keyword(s):  
Molecular Markers ◽  
Genetic Variability ◽  
Grape Variety

Distribution of dwarfing genes (Rht-B1b and Rht-D1b) in Martonvásár wheat breeding materials

2011 ◽  
Vol 59 (3) ◽  
pp. 249-254 ◽  
Author(s):  
G. Gulyás ◽  
Z. Bognár ◽  
L. Láng ◽  
M. Rakszegi ◽  
Z. Bedő
Keyword(s):  
Triticum Aestivum ◽  
Molecular Markers ◽  
Plant Height ◽  
Wheat Breeding ◽  
Minor Role ◽  
Dwarfing Genes ◽  
Breeding Programmes ◽  
A Minor ◽  
Positive Results ◽  
Marker Information

A total of 266 Martonvásár (Mv) wheat (Triticum aestivum L.) accessions, including varieties and advanced lines, were examined using the “Perfect” molecular markers to detect the Rht-B1b (formerly Rht1) and Rht-D1b (formerly Rht2) semi-dwarfing genes. The gene Rht-B1b was detected in a total of 221 (83.5%) accessions. The Rht-D1b allele was found in fewer accessions. Overall 24 genotypes (9%) contained this allele. The analysis of the development date of the genotypes revealed that the introduction of the dwarfing genes into Martonvásár breeding programmes started in the early 1970s, and they were widely utilized from the 1980s. The Rht-B1b allele was the main source for reducing plant height, while the Rht-D1b allele played only a minor role in the Martonvásár breeding programme.Characterizing accessions using various molecular markers allows us to create a database offering relevant marker information about genotypes. Such a database could be very helpful for selection, allowing breeders to include varieties giving positive results in specific breeding programmes.

scholarly journals In vitro Propagation and Assessment of Genetic Relationships of Citrus Rootstocks Using ISSR Molecular Markers

2017 ◽  
Vol 45 (2) ◽  
pp. 383-391 ◽  
Author(s):  
Constantinos SALIS ◽  
Ioannis E. PAPADAKIS ◽  
Spyridon KINTZIOS ◽  
Marianna HAGIDIMITRIOU
Keyword(s):  
Molecular Markers ◽  
Genetic Variability ◽  
Root Length ◽  
In Vitro Propagation ◽  
Genetic Relationships ◽  
Shoot Proliferation ◽  
Poncirus Trifoliata ◽  
Naphthaleneacetic Acid ◽  
Issr Molecular Markers

The behavior of six citrus rootstocks, Volkameriana, Citrumelo ‘Swingle’, Citrange ‘Carrizo’, Poncirus trifoliata ‘Serra’, Poncirus trifoliata ‘Rubidoux’ and Poncirus trifoliata ‘Flying Dragon’, in in vitro propagation was studied and compared for shoot proliferation and rooting. In addition, the genetic relationships among the rootstocks studied and other Citrus species, using the Inter-Simple Sequence Repeats (ISSR) molecular markers, were investigated. Nodal explants of three months old shoots were used in Murashige and Skoog medium supplemented with N6-benzyladenine (BA) for shoot proliferation and with naphthaleneacetic acid (NAA) for rooting. The rootstock Volkameriana showed a statistically significant higher number of shoots (1.81), shoot length (15.14 mm) and number of leaves per explant (5.81), while all three Poncirus trifoliata rootstocks showed the lowest numbers. The number of roots and root length per explant were evaluated at the end of the rooting phase. The rootstock ‘Swingle’ showed a higher number of roots per explant (4.2) followed by ‘Flying Dragon’ (3.93) and ‘Carrizo’ (3.23) rootstocks. The rootstocks ‘Swingle’ (140.8 mm), Volkameriana (148 mm) and ‘Flying Dragon’ (131.12 mm) had significantly higher root length per explant compared to ‘Carrizo’ (31 mm) and ‘Rubidoux’ (34.5 mm). The ISSR molecular marker technique used in the present study grouped successfully the different species, varieties and rootstocks studied, revealing their genetic variability. The genetic variability observed among the rootstocks ranged between 0.29 (Poncirus trifoliata ‘Serra’ and Citrumelo ‘Swingle’) and 0.60 (Volkameriana and Citrumelo ‘Swingle’). The response of the rootstocks studied in in vitro propagation however is not related to their genetic affinity.

scholarly journals Genetic variability in physic nuts cultivated in Northeastern Brazil

2013 ◽  
Vol 43 (6) ◽  
pp. 978-984 ◽  
Author(s):  
Vanice Dias Oliveira ◽  
Allivia Rouse Carregosa Rabbani ◽  
Ana Veruska Cruz da Silva ◽  
Ana da Silva Lédo
Keyword(s):  
Molecular Markers ◽  
Genetic Structure ◽  
Genetic Variability ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Dna Amplification ◽  
Northeastern Brazil ◽  
Jaccard Coefficient ◽  
Physic Nut ◽  
Genetic Structure And Diversity

This research had as objective to characterize genetically individuals of physic nut cultivated in experimental areas in Sergipe, Brazil by means of RAPD molecular markers. Leaves of 40 individuals were collected and DNA was isolated using CTAB 2% method. Were used 30 primers RAPD for DNA amplification, and this data was used to estimate the genetic similarity among the pairs of individuals, using Jaccard coefficient, and group them out for the UPGMA method. Also, the genetic structure and diversity of the populations were assessed using AMOVA. Of the 100 fragments generated, 29 of were polymorphic. A similarity average of 0.54 among the individuals was found and the amplitude similarities varied from 0.18 to 1.00. One of them (U5) was unit clusters and formed by the most divergent individuals. AMOVA indicated that there is more variation within (63%) the population. In conclusion, it was possible verify genetic variability in physic nut using RAPD markers at these experimental areas.

Sign in / Sign up

Export Citation Format

Share Document