BIOCHEMICAL STUDIES AND PARTIAL PURIFICATION OF CELLULASE ENZYME BIOSYNTHESIZED BY TRICHODERMA VIRIDE AND FUSARIUM OXYSPORIUM

Coffee pulp, which is waste material from coffee production line, has many poor natural degradeble property. Cellulase aillus nigernd pectinase, which were bio synthesized by the fugus races of Trichoderma viride and Asperg, can improve the disintergration process of coffee pulp. In this study, the conditions affecting the production of these enzymes were studied.. The optimum conditions for producing Pectinase enzyme of Aspergillus niger include: 2 days, 62% of humidity, 8% of culture. The optimum conditions for the production of Cellulase enzyme of Trichoderma viride are lasting for 4 days, 58% of humidity, 8% of culture. By carrying out real experiments we found that coffee pulp was disintergrated in the duration of 14 days, 60% of humidity, 8% of culture.

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Optimization of Cellulase Enzyme Production by Co-cultures of Fungi Isolated from Lignocellulosic Waste

International Journal for Modern Trends in Science and Technology - RTT2020 ◽

10.46501/ijmtst060504 ◽

2020 ◽

Vol 6 (5) ◽

pp. 19-26

Author(s):

Francis John V ◽

Dr. Soloman P A

Keyword(s):

Moisture Content ◽

Incubation Time ◽

Substrate Concentration ◽

Enzyme Production ◽

Trichoderma Viride ◽

Cellulase Production ◽

Optimal Conditions ◽

Lignocellulosic Waste ◽

Cellulase Enzyme ◽

Degree Of Degradation

Fruit wastes were incubated with the mixture of cellulolytic fungi Penicillium citrinum, Aspergillus oryzae, and Trichoderma viride to hydrolyze the cellulosic components and to increase the degree of degradation. . The batch experiments are statistically designed and performed using Box-Benhken method of Response Surface Methodology to investigate the influence of major parameters viz., incubation time, temperature, pH, moisture content and substrate concentration on cellulase enzyme production. Maximum cellulase production of 2.03 Units/ml (U/ml) was detected by the RSM method in a mixed culture containing fungi at a ratio of 1: 1: 1 under optimal conditions at an incubation time of 5.27 days, a temperature of 34.09 °C, pH 4.85, moisture content of 63.83% and a substrate concentration of 5.03%.

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BIOCHEMICAL STUDIES ON CYTOCHROME P-450 SOLUBILIZED FROM LIVER MICROSOMES: PARTIAL PURIFICATION AND MECHANISM OF CATALYSIS

Annals of the New York Academy of Sciences ◽

10.1111/j.1749-6632.1973.tb47613.x ◽

1973 ◽

Vol 212 (1 Multienzyme S) ◽

pp. 449-457 ◽

Cited By ~ 21

Author(s):

Minor J. Coon ◽

Robert M. Kaschnitz ◽

Henry W. Strobel ◽

Theodore A. Hoeven

Keyword(s):

Liver Microsomes ◽

Partial Purification ◽

Biochemical Studies ◽

Mechanism Of Catalysis ◽

Cytochrome P 450

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Antagonistic activity of cellulase enzyme produced by Trichoderma Viride against Xanthomonas Citr

Indian Journal of Agricultural Research ◽

10.18805/ijare.a-4999 ◽

2018 ◽

Author(s):

Prabhat Jatav ◽

Sonu Singh Ahirwar ◽

Abhishek Gupta ◽

Kirti Kushwaha ◽

Sadhna Jatav

Keyword(s):

Antibacterial Activity ◽

Soil Fungi ◽

Trichoderma Viride ◽

Citrus Canker ◽

Antibacterial Activities ◽

Control Agent ◽

Antagonistic Activity ◽

Bacterial Disease ◽

Cellulase Enzyme ◽

Citrus Disease

Citrus canker is a bacterial disease which occurs in citrus (lemon) species produce by Xanthomonas especially X. citri. The infection occurs on the lesions of leaves, stems, and fruits of citrus trees, it also occurs in other plants such as orange, grapes. The main goal of this study was to prevent the lemon (citrus) disease such as citrus canker by using some cellulase enzymes produces by soil fungi. In the present study, we isolated soil fungi Trichoderma viride, which produces cellulase enzyme these have higher antibacterial activities against plant pathogen such as X. citri. This enzyme produced by T. viride by using fermentation method, quantified by DNAS method and used as a bio-control agent against X. citri. They also isolated and characterized X. citri. In the last step of the study they have checked antibacterial activity of cellulase enzyme against X. citri. The present study concluded that cellulase enzyme produced by T. viride shows higher antibacterial activity against X. citri when treated it at 100°C.

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