scholarly journals Optimization of Cellulase Enzyme Production by Co-cultures of Fungi Isolated from Lignocellulosic Waste

2020 ◽  
Vol 6 (5) ◽  
pp. 19-26
Author(s):  
Francis John V ◽  
Dr. Soloman P A
Keyword(s):  
Moisture Content ◽  
Incubation Time ◽  
Substrate Concentration ◽  
Enzyme Production ◽  
Trichoderma Viride ◽  
Cellulase Production ◽  
Optimal Conditions ◽  
Lignocellulosic Waste ◽  
Cellulase Enzyme ◽  
Degree Of Degradation

Fruit wastes were incubated with the mixture of cellulolytic fungi Penicillium citrinum, Aspergillus oryzae, and Trichoderma viride to hydrolyze the cellulosic components and to increase the degree of degradation. . The batch experiments are statistically designed and performed using Box-Benhken method of Response Surface Methodology to investigate the influence of major parameters viz., incubation time, temperature, pH, moisture content and substrate concentration on cellulase enzyme production. Maximum cellulase production of 2.03 Units/ml (U/ml) was detected by the RSM method in a mixed culture containing fungi at a ratio of 1: 1: 1 under optimal conditions at an incubation time of 5.27 days, a temperature of 34.09 °C, pH 4.85, moisture content of 63.83% and a substrate concentration of 5.03%.

scholarly journals Cellulase Enzyme Production from Filamentous Fungi Trichoderma reesei and Aspergillus awamori in Submerged Fermentation with Rice Straw

2021 ◽  
Vol 7 (10) ◽  
pp. 868
Author(s):  
Laila Naher ◽  
Siti Noor Fatin ◽  
Md Abdul Halim Sheikh ◽  
Lateef Adebola Azeez ◽  
Shaiquzzaman Siddiquee ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Filamentous Fungi ◽  
Rice Straw ◽  
Submerged Fermentation ◽  
Enzyme Production ◽  
Dry Weight ◽  
Cellulase Production ◽  
Fungal Species ◽  
Aspergillus Awamori ◽  
Cellulase Enzyme

Fungi are a diverse group of microorganisms that play many roles in human livelihoods. However, the isolation of potential fungal species is the key factor to their utilization in different sectors, including the enzyme industry. Hence, in this study, we used two different fungal repositories—soil and weed leaves—to isolate filamentous fungi and evaluate their potential to produce the cellulase enzyme. The fungal strains were isolated using dichloran rose bengal agar (DRBA) and potato dextrose agar (PDA). For cellulase enzyme production, a rice straw submerged fermentation process was used. The enzyme production was carried out at the different incubation times of 3, 5, and 7 days of culture in submerged conditions with rice straw. Fungal identification studies by morphological and molecular methods showed that the soil colonies matched with Trichoderma reesei, and the weed leaf colonies matched with Aspergillus awamori. These species were coded as T. reesei UMK04 and A. awamori UMK02, respectively. This is the first report of A. awamori UMK02 isolation in Malaysian agriculture. The results of cellulase production using the two fungi incorporated with rice straw submerged fermentation showed that T. reesei produced a higher amount of cellulase at Day 5 (27.04 U/mg of dry weight) as compared with A. awamori (15.19 U/mg of dry weight), and the concentration was significantly different (p < 0.05). Our results imply that T. reesei can be utilized for cellulase production using rice straw.

scholarly journals OPTIMIZATION OF CELLULASE ENZYME PRODUCTION FROM Aspergillus oryzae FOR INDUSTRIAL APPLICATIONS

2017 ◽  
Vol 2 (2) ◽  
pp. 155 ◽  
Author(s):  
Hassan Sher ◽  
Muhammad Faheem ◽  
Abdul Ghani ◽  
Rashid Mehmood ◽  
Hamza Rehman ◽  
...  
Keyword(s):  
Enzyme Production ◽  
Time Course ◽  
Value Added ◽  
Cellulase Production ◽  
Industrial Applications ◽  
Inoculum Size ◽  
Cellulosic Biomass ◽  
High Yield ◽  
Carboxymethyl Cellulase ◽  
Cellulase Enzyme

Cellulases are the hydrolytic group of enzymes, responsible for release of sugars in the bioconversion of the cellulosic biomass into a variety of value added industrial products. Fungal isolated cellulases are well studied and playing a significant role in various industrial processes. Enzymatic depolymerisation of cellulosic material has been done by the various fungal isolated enzymes. In the present study, the cultivation conditions for cellulase production from Aspergillus species were optimized. Optimization of scarification conditions such as time course, inoculum size, carbon source and concentration, nitrogen source, various pH levels were performed for the production of extracellular carboxymethyl cellulase and endoglucanase enzyme. The result exhibited, 15 % inoculums size, corncobs 2 % concentration, Urea and medium pH 7 at 30oC supported high yield of carboxymethyl cellulase (38.80 U/ml/min) and exoglucanase enzyme (10.94 U/ml/min) through a submerged fermentation (SmF). In future biotechnological applications in cellulase enzyme production attain a vital role to obtain high degradable yield.

Optimization of Cellulase Production Using Agricultural Wastes by Artificial Neural Network and Genetic Algorithm

2011 ◽  
Vol 6 (1) ◽  
Author(s):  
Chun Chang ◽  
Guizhuan Xu ◽  
Junfang Yang ◽  
Duo Wang
Keyword(s):  
Neural Network ◽  
Genetic Algorithm ◽  
Artificial Neural Network ◽  
Solid State ◽  
Aspergillus Niger ◽  
Moisture Content ◽  
Trichoderma Viride ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Fermentation Time

The cellulase production by Trichoderma viride was optimized using artificial intelligence-based techniques under solid state fermentation. In this study, a back propagation network was designed with Levenberg-Marquardt training algorithm, and the tangent sigmoid and pure linear functions were used as the transfer functions in the hidden and output layers of the ANN, respectively. An artificial neural network coupling genetic algorithms was used to optimize the process parameters, which include the mass ratio of wheat straw to wheat bran, moisture content and fermentation time. The ultimate process parameters of optimization were mass ration of wheat straw to wheat bran 2.9, moisture content 69.6 percent, and fermentation time 123.3h. Further test experiment showed that the final cellulase activity can reach to 11.62 U/g, which was the highest value among all the experimental results. This result indicates that the genetic algorithm based on a neural network model is a better optimization method for cellulase production in solid state fermentation. To improve the cellulase production, a mixed culture system of Trichoderma viride and Aspergillus niger was also developed. The cellulase activity increased by 7.40 percent with the addition of Aspergillus niger at 72h.

scholarly journals Produksi Enzim Selulase Dari Bakteri Serratia marcescens KE-B6 Dengan Penambahan Sumber Karbon, Nitrogen dan Kalsium Pada Medium Produksi

2018 ◽  
Vol 19 (2) ◽  
pp. 159 ◽  
Author(s):  
Arom Septiani ◽  
W Wijanarka ◽  
MG Isworo Rukmi
Keyword(s):  
Serratia Marcescens ◽  
Growth Medium ◽  
Incubation Time ◽  
Enzyme Production ◽  
Production Medium ◽  
Cellulolytic Bacteria ◽  
Cellulase Enzyme ◽  
Randomized Design ◽  
Calcium Source ◽  
Calcium Sources

The waste of cellulose in the agro-industry can be reduced by decomposing the cellulose polymer into glucose. This process was carried out by cellulase enzyme (EC 3.2.1.4) produced by cellulolytic bacteria. Bacteria required food as nutrition to survived their life, can be obtained through growth medium or enzyme production medium. Carbon, nitrogen and calcium belong to the essential nutrients contained in growth medium and enzyme production medium. The purpose of this study is to determine the effect of the addition of carbon, nitrogen and calcium source and the time of incubation on the production of cellulase enzyme from Seratia marcescens KE-B6 bacteria. This research used Completely Randomized Design (RAL) of Factorial Pattern with two factors. The first factor is the type of medium, the first medium is the standard medium (M1) and the second medium is enriched with carbon, nitrogen and calcium sources (M2), the second factor is the incubation time with 5 repetitions. The enzyme production is measured by the reducing sugar method. The data obtained were analyzed using Anova. The results showed that the addition of carbon, nitrogen, and calcium sources and incubation time did not affect the production of cellulase enzyme by Serratia marcescens KE-B6. Keywords: Cellulose, Cellulase enzyme, Serratia marcescens

scholarly journals Potential of Aspergilus Niger MT809753 for Bio-treatment Paper Industry Wastewater After Screening Effective Factors on Production of Cellulase Enzyme by Plackett-burman Design

2021 ◽  
Author(s):  
Marwa El-Sesy ◽  
Amira M Aly
Keyword(s):  
Rice Straw ◽  
Enzyme Production ◽  
Paper Industry ◽  
Cellulase Production ◽  
Barley Straw ◽  
Cellulase Enzyme ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Aspergilus Niger ◽  
Industry Wastewater

Abstract A microorganism capable of degrading cellulose present in rice straw was isolated from wastewater samples and identified as Aspergilus niger MT809753 by 18S rDNA. In the present study various cheap agronomic cellulosic wastes as (cotton seed husks, barley straw, rice straw and maize straw) were utilized as crude inducers for the cellulase enzyme production and represent the carbon source for isolates where cellulose activity was measured by (DNS) method. The highest cellulases enzyme production was obtained by fungal isolate Aspergilus niger MT809753 within 24 hours (0.532 IU/ml) using rice straw. Plackett-Burman design was used as conventional method for statistically screening of different variables. Nine variables of the production process were selected. The results illustrate those seven variables, namely as (inoculum size, substrate concentration, incubation temperature, pH, shaking conditions, and incubation time and peptone concentration) had influence with high confidence levels, while the remaining two variables did not show a significant effect on cellulase production. After using response optimization the experiment was performed and the obtained cellulase production was 1.08 IU/ml. A bench scale study was performed to examine paper industry wastewater treatment efficiency by Aspergillus Niger MT809753. Results reveal that organisms have proved their bioremediation potency in treatment of paper industry effluent. The importance of the research stems from the fact that it sheds light on the role of some fungi in the production of the cellulase enzyme. So our goal is to obtain local isolates from fungi having a high ability to produce the cellulase enzyme, as well as developing an effective treatment processes to get rid of environmental cellulosic pollution and utilization of cellulosic wastes as cheap carbon sources.

scholarly journals Optimization of fermentation condition for cellulase enzyme production from Bacillus sp.

2019 ◽  
Vol 2 (2) ◽  
pp. 19-24
Author(s):  
Maidul Islam ◽  
Palash Kumar Sarkar ◽  
A.K.M. Mohiuddin ◽  
Md. Suzauddula
Keyword(s):  
Enzyme Production ◽  
Production Condition ◽  
Fermentation Medium ◽  
Cellulase Production ◽  
Shake Flask Culture ◽  
Industrial Biotechnology ◽  
Bacillus Sp ◽  
Cellulase Enzyme ◽  
Optimization Of Fermentation

AbstractCellulase is an important enzyme in present-day of industrial biotechnology. The current study is concerned with the production and partial characterization of cellulase enzyme from Bacillus sp. The effect of various fermentation conditions for cellulase production through shake-flask culture was investigated. Maximum enzyme production was obtained after 24 hours of incubation in fermentation medium with pH 3.5 at 35°C under having agitation at 150 rpm while inoculums volume 1% was applied. Enzyme production was 1.91 times higher after optimizing the production condition as compared to the basal media. Cellulase characterization revealed that optimum activity was at pH 5.5 and 50°C for 50 minutes. About 68% of the activity retained after heating the crude enzyme solution at 50°C for 30 minutes. This nature makes cellulase a suitable candidate for current mainstream biomass conversions for sustainable agriculture and industrial processes.

scholarly journals Cellulase enzyme production enhancement in Trichoderma viride by Gamma induced mutation

2018 ◽  
Vol 4 (4) ◽  
pp. 329-336
Author(s):  
Khadijeh Bagheri ◽  
Samira Shahbazi ◽  
Hamed Askari ◽  
Shide Mojerlou ◽  
Farangis Amirlou ◽  
...  
Keyword(s):  
Enzyme Production ◽  
Trichoderma Viride ◽  
Induced Mutation ◽  
Cellulase Enzyme

scholarly journals PRODUKSI DAN PENENTUAN KONDISI OPTIMUM ENZIM XILANASE Bacillus amyloliquefaciens FUKUMOTO PADA SUBSTRAT XILAN JERAMI

2015 ◽  
Vol 2 (1) ◽  
pp. 74
Author(s):  
Widiyanti Sekatresna ◽  
Abdi Dharma ◽  
Periadnadi
Keyword(s):  
Enzyme Activity ◽  
Rice Straw ◽  
Incubation Time ◽  
Substrate Concentration ◽  
Bacillus Amyloliquefaciens ◽  
Specific Activity ◽  
Enzymatic Reaction ◽  
Optimal Conditions ◽  
Enzyme Specific Activity

 ABSTRACT The production and determination of  optimal condition of xylanase produced by Bacillus amyloliquefaciens on rice straw xylan were investigated in this study. The parameters to be observed were optimal conditions of pH, temperature, substrate concentration and incubation time. Xilanase activity was determined by measuring the amount of reducing sugar formed in the enzymatic reaction based on Somogyi Nelson method. Optimal conditions needed for the production of xylanase were at pH 7, temperature 27°C and six days of incubation time. While optimal conditions of xylanase action were reached at pH 8.2, temperature 45°C, substrate concentration 3.5%(w/w) and 15 minutes of incubation time with enzyme activity and enzyme specific activity of 1.285 U/mL and 0.738 U/mg respectively. As a comparison, xylanase was also produced on pure xylan  (birchwood), enzyme activity and enzyme specific activity obtained were 2.701 U/mL and 1.658 U/mg respectively. Cellulase content in enzyme produced on rice straw xilan showed the enzyme activity of 0.094 U/mL.  Keywords : xylanase, Bacillus amyloliquefaciens, rice straw xilan

PRODUCTION OF LIGNOCELLULOSES FROM ASPERGILLUS NIGER USING ARECA NUT SPADE AS BIOMASS..

2019 ◽  
pp. 1-3
Author(s):  
Madhuri B ◽  
Narasimha G ◽  
Balaji M*
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Cellulase Production ◽  
Areca Nut ◽  
Physical Treatment ◽  
Cellulose Production ◽  
Lactobionic Acid ◽  
Cellulase Enzyme

Areca palm (ChrysalidoCarpus lutescenes) a widely used plant having feathery arching brands with 100 leaflets. All these plants produce much of waste in additions to greeny and nuts. This waste of spade is used for the production of various molecules that are used in industry and pharma sector. Fermentation techniques are used to generate economically important enzymes for industrial and pharmaceutical purposes. Cellulase enzyme degrades the cellulose in between β-1, 4 glucosidic link found in lignocellulosic complex which under physical treatment is slower to degrade. The present study of Aspergillus niger for cellulose production was carried in solid state (SS) and submerged (SM) fermentations for production of cellulase enzyme. Cellulase production in SSF after 72 h of fermentation was 8.02 and in SMF activity was 2.98 per ml of cultured broth at H 6 and temperature at 30°C. Both SMF and SSF were supplemented with lactose and lactobionic acid, which acted as cellulase P production inducers. The aim of the present work was to study the effect of Areca palm spade as substrate for Aspergillus niger and its cellulase production under SMF and SSF.

Sign in / Sign up

Export Citation Format

Share Document