scholarly journals Distinct Metal Ion Requirements for the Phosphomonoesterase and Phosphodiesterase Activities of Calf Intestinal Alkaline Phosphatase

2011 ◽  
Vol 5 ◽  
pp. 67-72 ◽  
Author(s):  
Adedoyin Igunnu ◽  
Dunsin S Osalaye ◽  
Olufunso O Olorunsogo ◽  
Sylvia O Malomo ◽  
Femi J Olorunniji
Keyword(s):  
Alkaline Phosphatase ◽  
Metal Ion ◽  
Intestinal Alkaline Phosphatase ◽  
Calf Intestinal Alkaline Phosphatase

The Calf Intestinal Alkaline Phosphatase. II. Reaction between the metal content and the enzyme activity

1983 ◽  
Vol 66 (3) ◽  
pp. 871-879 ◽  
Author(s):  
Plato Portmann ◽  
Hubert Schaller ◽  
Geneviève Leva ◽  
Werner Venetz ◽  
Thomas Müller
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Metal Content ◽  
Intestinal Alkaline Phosphatase ◽  
Calf Intestinal Alkaline Phosphatase

Structural study on the carbohydrate moiety of calf intestinal alkaline phosphatase

2001 ◽  
Vol 36 (8) ◽  
pp. 960-972 ◽  
Author(s):  
Renate Bublitz ◽  
Horst Hoppe ◽  
Gerhard A. Cumme ◽  
Mariana Thiele ◽  
Adrian Attey ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Structural Study ◽  
Carbohydrate Moiety ◽  
Intestinal Alkaline Phosphatase ◽  
Calf Intestinal Alkaline Phosphatase

Calf Intestinal Alkaline Phosphatase I. Improved Isolation Method and Molar Composition of the Purified Phosphatase

1982 ◽  
Vol 65 (8) ◽  
pp. 2668-2681 ◽  
Author(s):  
Plato Portmann ◽  
Andreas Jörg ◽  
Kurt Furrer ◽  
Hans-Sepp Walker ◽  
Peter Leuthard ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Isolation Method ◽  
Intestinal Alkaline Phosphatase ◽  
Calf Intestinal Alkaline Phosphatase ◽  
Molar Composition

scholarly journals Inhibition of endogenous tissue alkaline phosphatase with the use of alkaline phosphatase conjugates in immunohistochemistry.

1981 ◽  
Vol 29 (8) ◽  
pp. 981-984 ◽  
Author(s):  
B A Ponder ◽  
M M Wilkinson
Keyword(s):  
Alkaline Phosphatase ◽  
Acetic Acid ◽  
Frozen Section ◽  
Intestinal Alkaline Phosphatase ◽  
Calf Intestinal Alkaline Phosphatase ◽  
Immunohistochemical Demonstration ◽  
Alkaline Phosphatase Staining ◽  
Mouse Tissues

In mammals there are two forms of alkaline phosphatase, one of which is widely distributed in a variety of tissues, and one of which is confined to intestine. Levamisole (1-tetramisole) inhibits the nonintestinal form of the enzyme, but is without effect on the intestinal form. We have exploited this difference by using conjugates made with calf intestinal alkaline phosphatase for immunohistochemical demonstration of H2 antigens in frozen section of mouse tissues. The alkaline phosphatase staining is performed in the presence of 1 mm levamisole, which inhibits the endogenous tissue enzyme without loss of staining by the conjugate. Endogenous enzyme can be inhibited by other means, such as exposure to 20% acetic acid, but labile antigens may be destroyed.

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