In vivo Heterotopic Bone Formation Assay Using Isolated Mouse and Human Mesenchymal Stem Cells

BIO-PROTOCOL ◽  
2015 ◽  
Vol 5 (3) ◽  
Author(s):  
Li Chen ◽  
Nicholas Ditzel
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Human Mesenchymal Stem Cells ◽  
Heterotopic Bone ◽  
Heterotopic Bone Formation

Chondrogenic pre-induction of human mesenchymal stem cells on β-TCP: Enhanced bone quality by endochondral heterotopic bone formation

2010 ◽  
Vol 6 (8) ◽  
pp. 3292-3301 ◽  
Author(s):  
Patricia Janicki ◽  
Philip Kasten ◽  
Kerstin Kleinschmidt ◽  
Reto Luginbuehl ◽  
Wiltrud Richter
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Bone Quality ◽  
Human Mesenchymal Stem Cells ◽  
Heterotopic Bone ◽  
Heterotopic Bone Formation

scholarly journals Canonical Wnts function as potent regulators of osteogenesis by human mesenchymal stem cells

2009 ◽  
Vol 185 (1) ◽  
pp. 67-75 ◽  
Author(s):  
Guizhong Liu ◽  
Sapna Vijayakumar ◽  
Luca Grumolato ◽  
Randy Arroyave ◽  
HuiFang Qiao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Wnt Signaling ◽  
De Novo ◽  
Human Mesenchymal Stem Cells ◽  
Mitogen Activated Protein Kinase ◽  
Bone Homeostasis

Genetic evidence indicates that Wnt signaling is critically involved in bone homeostasis. In this study, we investigated the functions of canonical Wnts on differentiation of adult multipotent human mesenchymal stem cells (hMSCs) in vitro and in vivo. We observe differential sensitivities of hMSCs to Wnt inhibition of osteogenesis versus adipogenesis, which favors osteoblastic commitment under binary in vitro differentiation conditions. Wnt inhibition of osteogenesis is associated with decreased expression of osteoblastic transcription factors and inhibition of c-Jun N-terminal kinase and p38 mitogen-activated protein kinase activation, which are involved in osteogenic differentiation. An hMSC subpopulation exhibits high endogenous Wnt signaling, the inhibition of which enhances osteogenic and adipogenic differentiation in vitro. In an in vivo bone formation model, high levels of Wnt signaling inhibit de novo bone formation by hMSCs. However, hMSCs with exogenous expression of Wnt1 but not stabilized β-catenin markedly stimulate bone formation by naive hMSCs, arguing for an important role of a canonical Wnt gradient in hMSC osteogenesis in vivo.

Activation of the cAMP/PKA pathway in human mesenchymal stem cells in vitro results in robust bone formation in vivo

Bone ◽  
2008 ◽  
Vol 42 ◽  
pp. S30
Author(s):  
R. Siddappa ◽  
J. Doorn ◽  
A. Leusink ◽  
C. van Blitterswijk ◽  
J. de Boer
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Human Mesenchymal Stem Cells ◽  
Pka Pathway
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