scholarly journals Bone formation of human mesenchymal stem cells harvested from reaming debris is stimulated by low-dose bone morphogenetic protein-7 application in vivo

2016 ◽  
Vol 13 (4) ◽  
pp. 404-408 ◽  
Author(s):  
Fabian Westhauser ◽  
Melanie Höllig ◽  
Bruno Reible ◽  
Kai Xiao ◽  
Gerhard Schmidmaier ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Bone Morphogenetic Protein ◽  
Low Dose ◽  
Human Mesenchymal Stem Cells ◽  
Bone Morphogenetic Protein 7 ◽  
Morphogenetic Protein

scholarly journals Canonical Wnts function as potent regulators of osteogenesis by human mesenchymal stem cells

2009 ◽  
Vol 185 (1) ◽  
pp. 67-75 ◽  
Author(s):  
Guizhong Liu ◽  
Sapna Vijayakumar ◽  
Luca Grumolato ◽  
Randy Arroyave ◽  
HuiFang Qiao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Wnt Signaling ◽  
De Novo ◽  
Human Mesenchymal Stem Cells ◽  
Mitogen Activated Protein Kinase ◽  
Bone Homeostasis

Genetic evidence indicates that Wnt signaling is critically involved in bone homeostasis. In this study, we investigated the functions of canonical Wnts on differentiation of adult multipotent human mesenchymal stem cells (hMSCs) in vitro and in vivo. We observe differential sensitivities of hMSCs to Wnt inhibition of osteogenesis versus adipogenesis, which favors osteoblastic commitment under binary in vitro differentiation conditions. Wnt inhibition of osteogenesis is associated with decreased expression of osteoblastic transcription factors and inhibition of c-Jun N-terminal kinase and p38 mitogen-activated protein kinase activation, which are involved in osteogenic differentiation. An hMSC subpopulation exhibits high endogenous Wnt signaling, the inhibition of which enhances osteogenic and adipogenic differentiation in vitro. In an in vivo bone formation model, high levels of Wnt signaling inhibit de novo bone formation by hMSCs. However, hMSCs with exogenous expression of Wnt1 but not stabilized β-catenin markedly stimulate bone formation by naive hMSCs, arguing for an important role of a canonical Wnt gradient in hMSC osteogenesis in vivo.

Human Mesenchymal Stem Cells Transduced with Recombinant Bone Morphogenetic Protein-9 Adenovirus Promote Osteogenesis in Rodents

2003 ◽  
Vol 9 (2) ◽  
pp. 347-356 ◽  
Author(s):  
Hayan Dayoub ◽  
Randall J. Dumont ◽  
Jin Zhong Li ◽  
Aaron S. Dumont ◽  
Gerald R. Hankins ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Morphogenetic Protein ◽  
Human Mesenchymal Stem Cells ◽  
Morphogenetic Protein ◽  
Bone Morphogenetic Protein 9
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