scholarly journals Поляризованная флуоресценция молекул NADH при двухфотонном возбуждении фемтосекундными лазерными импульсами с длиной волны 720-780 nm

2019 ◽  
Vol 45 (13) ◽  
pp. 37
Author(s):  
М.Э. Сасин ◽  
И.А. Горбунова ◽  
Н.O. Безверхний ◽  
Я.М. Бельтюков ◽  
J. Rubayo-Soneira ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Single Photon ◽  
Laser Pulses ◽  
Time Correlation ◽  
Femtosecond Laser Pulses ◽  
Diffusion Time ◽  
Methanol Solution ◽  
Polarized Fluorescence ◽  
Two Photon ◽  
Photon Excitation

The decay of polarized fluorescence in coenzyme NADH upon two-photon excitation by femtosecond laser pulses at the wavelength range of 720- 780 nm in water/methanol solution has been investigated. Decay fluorescence times tau_1 and tau_2, rotation diffusion time tau_rot, and preexponential factors a_1/a_2 ratio have been determined from experiment. The results obtained have been compared with previous results in NADH in aqueous solution. polarized fluorescence, NADH, adenine, nicotinamide, spectroscopy, time correlation single photon spectroscopy

Polarized Fluorescence in Indole under Two-Photon Excitation by Femtosecond Laser Pulses

2018 ◽  
Vol 63 (9) ◽  
pp. 1293-1299
Author(s):  
M. E. Sasin ◽  
V. I. Tushkanov ◽  
A. G. Smolin ◽  
P. S. Shternin ◽  
O. S. Vasyutinskii
Keyword(s):  
Femtosecond Laser ◽  
Laser Pulses ◽  
Femtosecond Laser Pulses ◽  
Polarized Fluorescence ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Picosecond Single-Photon and Femtosecond Two-Photon Pulsed Laser Stimulation for IC Characterization and Failure Analysis

2009 ◽  
Author(s):  
V. Pouget ◽  
E. Faraud ◽  
K. Shao ◽  
S. Jonathas ◽  
D. Horain ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Single Photon ◽  
Pulsed Laser ◽  
Laser Pulses ◽  
Femtosecond Laser Pulses ◽  
Ultrashort Laser Pulses ◽  
Laser Stimulation ◽  
Two Photon ◽  
Resolution Improvement ◽  
Ultrashort Laser

Abstract This paper presents the use of pulsed laser stimulation with picosecond and femtosecond laser pulses. We first discuss the resolution improvement that can be expected when using ultrashort laser pulses. Two case studies are then presented to illustrate the possibilities of the pulsed laser photoelectric stimulation in picosecond single-photon and femtosecond two-photon modes.

Polarized Fluorescence in NADH Two-Photon Excited by Femtosecond Laser Pulses in the Wavelength Range of 720–780 nm

2019 ◽  
Vol 45 (7) ◽  
pp. 672-674 ◽  
Author(s):  
M. E. Sasin ◽  
I. A. Gorbunova ◽  
N. O. Bezverkhnii ◽  
Y. M. Beltukov ◽  
O. S. Vasyutinskii ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Wavelength Range ◽  
Laser Pulses ◽  
Femtosecond Laser Pulses ◽  
Polarized Fluorescence ◽  
Two Photon

scholarly journals Two-Color Two-Photon Excitation Using Femtosecond Laser Pulses

2008 ◽  
Vol 112 (18) ◽  
pp. 5768-5773 ◽  
Author(s):  
Stefan Quentmeier ◽  
Stefan Denicke ◽  
Jan-Eric Ehlers ◽  
Raluca A. Niesner ◽  
Karl-Heinz Gericke
Keyword(s):  
Femtosecond Laser ◽  
Laser Pulses ◽  
Femtosecond Laser Pulses ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Femtosecond Two-Photon 3D Lightfield Lithography

2021 ◽  
Author(s):  
Aravind Jakkinapalli ◽  
Balaji Baskar ◽  
Sy-Bor Wen
Keyword(s):  
Spatial Light Modulator ◽  
Light Field ◽  
Single Photon ◽  
Microlens Array ◽  
Laser Pulses ◽  
Femtosecond Laser Pulses ◽  
Virtual Image ◽  
Light Modulator ◽  
Two Photon ◽  
Uv Led

Abstract Based on the successful single-photon 3D light field photolithography we demonstrated in the last year, we extend the methodology to femtosecond 3D light field lithography. Compared with our previous single-photon work with UV LED light, using femtosecond light and the associated two-photon light absorption in 3D light field lithography can cure photoresist only around designed voxel locations in a 3D space. Such a two-photon scheme can prevent the unwilling curing of photoresists along the optical paths of rays before arriving at designed voxel locations, which is observed in our previous UV LED-based single-photon 3D light field lithography. The experimental scheme of femtosecond two-photon 3D light field lithography starts from delivering uniform femtosecond laser pulses to a spatial light modulator. The designed pixel map is presented on the spatial light modulator and then delivered to a microlens array to construct a 3D virtual image in the free space. By compressing the 3D virtual image in a photoresist layer with a microscope system, we can successfully generate different microscale 3D patterns without relying on scanning processes as in traditional 3D lithography. In this study, we present preliminary results of (a) algorithms developed to generated 3D patterns with femtosecond light, which should satisfy additional constraints when femtosecond light is used, and (b) 3D patterns generated in photoresists with femtosecond two-photon 3D light field lithography.

scholarly journals Определение времен затухания и анизотропии поляризованной флуоресценции флавинадениндинуклеотида с субнаносекундным разрешением

2020 ◽  
Vol 46 (12) ◽  
pp. 43
Author(s):  
М.К. Краснопевцева ◽  
В.П. Белик ◽  
А.А. Богданов ◽  
И.В. Семенова ◽  
А.Г. Смолин ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Flavin Adenine Dinucleotide ◽  
Rotational Diffusion ◽  
Anisotropy Parameter ◽  
Picosecond Laser ◽  
Laser Pulses ◽  
Diffusion Time ◽  
Fluorescence Lifetimes ◽  
Polarized Fluorescence ◽  
Time Resolved

Optical properties of the biological coenzyme flavin adenine dinucleotide (FAD) in an aqueous solution were investigated. The time-resolved decay of polarized fluorescence excited by picosecond laser pulses provided data on two fluorescence lifetimes, rotational diffusion time and anisotropy parameter. The results obtained are compared with those obtained by other researchers.

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