Temporal control of the integrated stress response by a stochastic molecular switch

Stress granules (SGs) are formed in the cytosol as an acute response to environmental cues and activation of the integrated stress response (ISR), a central signaling pathway controlling protein synthesis. Using chronic virus infection as stress model, we previously uncovered a unique temporal control of the ISR resulting in recurrent phases of SG assembly and disassembly. Here, we elucidate the molecular network generating this fluctuating stress response, by integrating quantitative experiments with mathematical modeling, and find that the ISR operates as a stochastic switch. Key elements controlling this switch are the cooperative activation of the stress-sensing kinase PKR, the ultrasensitive response of SG formation to the phosphorylation of the translation initiation factor eIF2alpha, and negative feedback via GADD34, a stress-induced subunit of protein phosphatase 1. We identify GADD34 mRNA levels as the molecular memory of the ISR that plays a central role in cell adaptation to acute and chronic stress.

2D scanning system of the acousto-optical deflector with high diffraction efficiency

The paper considers advantages and special features of an acousto-optic method for the development of a functional device for spatial and temporal control of laser light and reports results of investigations to create a two-directional acousto-optic laser scanner with diffraction efficiency as high as 80 percent or better. An acousto-optic deflector on a paratellurite crystal operating under the Bragg diffraction condition with a lithium niobate piezoelectric acoustic transducer was developed and tested experimentally. The results of this work can serve as a basis for the development of prototype functional acousto-optic devices for spatial and temporal control of laser light and their application to create laser television images.

Spatial and temporal control of expression with light-gated LOV-LexA

The ability to drive expression of exogenous genes in different tissues and cell types, under control of specific enhancers, has catapulted discovery in biology. While many enhancers drive expression broadly, several genetic tricks have been developed to obtain access to isolated cell types. However, studies of topographically organized neuropiles, such as the optic lobe in fruit flies, have raised the need for a system that can access subsets of cells within a single neuron type, a feat currently dependent on stochastic flip-out methods. To access the same subsets of cells consistently across flies, we developed LOV-LexA, a light-gated expression system based on the bacterial LexA transcription factor and the plant-derived LOV photosensitive domain. Expression of LOV-Lex in larval fat body as well as pupal and adult neurons enables spatial and temporal control of expression of transgenes under LexAop sequences with blue light. The LOV-LexA tool thus provides another layer of intersectional genetics, allowing for light-controlled genetic access to the same subsets of cells within an expression pattern across individual flies.

The Development of Ru(II)-Based Photoactivated Chemotherapy Agents

Photoactivated chemotherapy (PACT) is a novel cancer treatment method that has drawn increasing attention due to its high selectivity and low side effects by spatio-temporal control of irradiation. Compared with photodynamic therapy (PDT), oxygen-independent PACT is more suitable for treating hypoxic tumors. By finely tuning ligand structures and coordination configurations, many Ru(II) complexes can undergo photoinduced ligand dissociation, and the resulting Ru(II) aqua species and/or free ligands may have anticancer activity, showing their potential as PACT agents. In this mini-review, we summarized the progress in Ru(II)-based PACT agents, as well as challenges that researchers in this field still face.

TOWARDS A MULTI-DIMENSIONAL THEORY OF TEMPORAL CONTROL IN THE GIG ECONOMY

This paper proposes a multi-dimensional theory of temporal control in the gig economy. Specifically, we focus on different types of platform-based temporal control and their respective effects on (a) workers’ autonomy, (b) value of free time, and (c) their social and political connectedness. Theoretically, we draw on the sociology of time and normative time literature in political theory. Empirically, the framework is informed by the synthesis of the literature about algorithms in the gig economy, with particular attention to the methods of temporal control. Meanwhile, we draw insights from a larger comparative project that examines algorithmic labor control and resistance across ride-hailing platforms (Uber and Lyft), TaskRabbit, and delivery platforms (DoorDash, Uber Eats, and Instacart) in the United States. The project interviewed 50 gig workers between 2017 and 2020. Through a systematic synthesis of primary and secondary materials, this paper contributes to understanding temporalities and work autonomy in the gig economy, and more broadly, flexible workplaces where the boundaries between work and non-work time become blurred.