A comparative study on K562 cell ferritin and normal human liver ferritin.

We describe a method for the purification of normal human liver ferritin by ultrafiltration, gel filtration on Sephacryl S-300, and affinity chromatography on DEAE-Affi Gel Blue. The purity of the ferritin obtained was verified by immunoelectrophoresis, Ouchterlony immunodiffusion, polyacrylamide gel electrophoresis, and electrofocusing. This rapid method yields 32% of the original ferritin.

Download Full-text

Comparative Study of Human Liver Ferritin and Chicken Liver by Mössbauer Spectroscopy. Preliminary Results

ICAME 2003 ◽

10.1007/978-1-4020-2852-6_42 ◽

2004 ◽

pp. 279-284

Author(s):

M. I. Oshtrakh ◽

O. B. Milder ◽

V. A. Semionkin ◽

P. G. Prokopenko ◽

L. I. Malakheeva

Keyword(s):

Mössbauer Spectroscopy ◽

Comparative Study ◽

Human Liver ◽

Mossbauer Spectroscopy ◽

Chicken Liver ◽

Preliminary Results ◽

Mößbauer Spectroscopy ◽

Human Liver Ferritin ◽

Liver Ferritin

Download Full-text

Structural variations of the iron cores in human liver ferritin and its pharmaceutically important models: A comparative study using Mössbauer spectroscopy with a high velocity resolution

Journal of Molecular Structure ◽

10.1016/j.molstruc.2010.10.045 ◽

2011 ◽

Vol 993 (1-3) ◽

pp. 287-291 ◽

Cited By ~ 18

Author(s):

M.I. Oshtrakh ◽

I.V. Alenkina ◽

S.M. Dubiel ◽

V.A. Semionkin

Keyword(s):

Mössbauer Spectroscopy ◽

Comparative Study ◽

High Velocity ◽

Human Liver ◽

Mossbauer Spectroscopy ◽

Structural Variations ◽

High Velocity Resolution ◽

Mößbauer Spectroscopy ◽

Human Liver Ferritin ◽

Liver Ferritin

Download Full-text

Comparative study of nanosized iron cores in human liver ferritin and its pharmaceutically important models Maltofer® and Ferrum Lek using Mössbauer spectroscopy

Bulletin of the Russian Academy of Sciences Physics ◽

10.3103/s1062873813060038 ◽

2013 ◽

Vol 77 (6) ◽

pp. 739-744 ◽

Cited By ~ 5

Author(s):

I. V. Alenkina ◽

M. I. Oshtrakh ◽

V. A. Semionkin ◽

E. Kuzmann

Keyword(s):

Mössbauer Spectroscopy ◽

Comparative Study ◽

Human Liver ◽

Mossbauer Spectroscopy ◽

Mößbauer Spectroscopy ◽

Human Liver Ferritin ◽

Ferrum Lek ◽

Liver Ferritin ◽

Nanosized Iron

Download Full-text

Comparative Study of Human Liver Ferritin and Chicken Liver by Mössbauer Spectroscopy. Preliminary Results

Hyperfine Interactions ◽

10.1023/b:hype.0000043241.60542.43 ◽

2004 ◽

Vol 156/157 (1-4) ◽

pp. 279-284 ◽

Cited By ~ 8

Author(s):

M. I. Oshtrakh ◽

O. B. Milder ◽

V. A. Semionkin ◽

P. G. Prokopenko ◽

L. I. Malakheeva

Keyword(s):

Mössbauer Spectroscopy ◽

Comparative Study ◽

Human Liver ◽

Mossbauer Spectroscopy ◽

Chicken Liver ◽

Preliminary Results ◽

Mößbauer Spectroscopy ◽

Human Liver Ferritin ◽

Liver Ferritin

Download Full-text

Mössbauer spectroscopy of the iron cores in human liver ferritin, ferritin in normal human spleen and ferritin in spleen from patient with primary myelofibrosis: preliminary results of comparative analysis

BioMetals ◽

10.1007/s10534-012-9602-2 ◽

2013 ◽

Vol 26 (2) ◽

pp. 229-239 ◽

Cited By ~ 13

Author(s):

M. I. Oshtrakh ◽

I. V. Alenkina ◽

A. V. Vinogradov ◽

T. S. Konstantinova ◽

E. Kuzmann ◽

...

Keyword(s):

Mössbauer Spectroscopy ◽

Comparative Analysis ◽

Human Liver ◽

Mossbauer Spectroscopy ◽

Primary Myelofibrosis ◽

Human Spleen ◽

Preliminary Results ◽

Human Liver Ferritin ◽

Normal Human ◽

Liver Ferritin

Download Full-text

Comparative study of the iron cores in human liver ferritin, its pharmaceutical models and ferritin in chicken liver and spleen tissues using Mössbauer spectroscopy with a high velocity resolution

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy ◽

10.1016/j.saa.2012.02.083 ◽

2013 ◽

Vol 100 ◽

pp. 88-93 ◽

Cited By ~ 22

Author(s):

I.V. Alenkina ◽

M.I. Oshtrakh ◽

Yu.V. Klepova ◽

S.M. Dubiel ◽

N.V. Sadovnikov ◽

...

Keyword(s):

Mössbauer Spectroscopy ◽

Comparative Study ◽

High Velocity ◽

Human Liver ◽

Mossbauer Spectroscopy ◽

Chicken Liver ◽

High Velocity Resolution ◽

Human Liver Ferritin ◽

Liver Ferritin ◽

Chicken Liver And Spleen

Download Full-text

Influence of Liver Isoferritin Profile on Quantitative Measurement of Total Ferritin Protein in Human Liver, with Emphasis on Iron Overload

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/000456328302000204 ◽

1983 ◽

Vol 20 (2) ◽

pp. 80-85 ◽

Cited By ~ 1

Author(s):

F M J Zuyderhoudt ◽

C Linthorst ◽

G G A Jörning ◽

N C J J Ladiges ◽

A M Brugman

Keyword(s):

Iron Overload ◽

Nitrogen Content ◽

Isoelectric Focusing ◽

Human Liver ◽

Quantitative Measurement ◽

Immunological Methods ◽

Gel Chromatography ◽

Normal Human Liver ◽

Normal Human ◽

Liver Ferritin

Ferritin was isolated from normal human liver and from iron-loaded human liver by gel chromatography and by ultracentrifugation. From each of these ferritin batches several isoferritin fractions were isolated by preparative isoelectric focusing. It was our aim to have at our disposal isoferritin fractions with relatively large pI ranges but distinctly different isoferritin profiles on analytical isoelectric focusing. These fractions were compared for their immunoreactivity. The total protein in the isoferritin fractions was measured by the nitrogen content. The immunoreactivity of the isoferritin fractions was measured as the ratio of the reaction in immunoassays to the nitrogen content of these fractions. We used radial immunodiffusion and enzyme-linked immunoassay to measure immunoreactivity. The immunoreactivity did not change obviously with the isoferritin composition of the isolated fractions. It is concluded that pathological changes in the isoferritin composition that might occur in liver ferritin during iron overload does not significantly influence the quantitative measurement of liver ferritin protein by immunological methods.

Download Full-text