scholarly journals DETERMINATION OF PHYTOCOMPONENTS IN ETHANOL EXTRACT OF BRASSICA OLERACEA - USING GAS CHROMATOGRAPHY–MASS SPECTROSCOPY TECHNIQUE

2018 ◽  
Vol 11 (10) ◽  
pp. 133
Author(s):  
Jayalakshmi M ◽  
Vanitha V ◽  
Sangeetha R
Keyword(s):  
Gas Chromatography ◽  
Brassica Oleracea ◽  
Mass Spectroscopy ◽  
Ethanol Extract ◽  
Bioactive Components ◽  
Hexadecanoic Acid ◽  
Spectroscopy Technique ◽  
Medical Field ◽  
Gas Chromatography Mass Spectroscopy

Objectives: Bioactive components determined by plants are known to have a broad application in the medical field. The focus of this study is to recognize the phytochemicals in the ethanol extract of Brassica oleracea by gas chromatography–mass spectroscopy (GC–MS). Methods: B. oleracea was collected, dried, and powdered well. The extraction was done with the solvent ethanol. The extract was exposed to column of GC-MS-QP 2010 (SHIMADZU) column Db 30.0 (0.25 μm in diameter, 0.25 μm thick). Results: GC–MS result provides the chromatogram with different peaks obtained at a different retention time shows the presence of various biocompounds. Some of the identified bioactive compounds are n-hexadecanoic acid (12.99%), phytol (2.40%), Vitamin E (3.38%), tetratetracontane (2.15%), stigmasterol (2.03%), and isophytol. Conclusion: The GC–MS study of the ethanol extract of B. oleracea reveals the existence of many potential compounds that can be utilized in the pharmaceutical industry, including the use of anti-inflammatory, antiarthritic, anticoronary, and antidiabetic agents.

scholarly journals PHYTOCHEMICAL SCREENING AND GAS CHROMATOGRAPHY-MASS SPECTROSCOPY ANALYSIS OF BIOACTIVE COMPOUNDS AND BIOSYNTHESIS OF SILVER NANOPARTICLES USING SPROUT EXTRACTS OF VIGNA RADIATA L. AND THEIR ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY

2019 ◽  
pp. 180-184
Author(s):  
SIVAKUMAR T
Keyword(s):  
Gas Chromatography ◽  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Mass Spectroscopy ◽  
Vigna Radiata ◽  
Green Gram ◽  
Hexadecanoic Acid ◽  
Gas Chromatography Mass Spectroscopy

Objectives: The present study was aimed to investigate the facile synthesis of silver nanoparticles (AgNPs) using the green gram sprout extract (GGSE) of Vigna radiata L. and also in vitro studies of antioxidant and antimicrobial activities. Methods: Gas chromatography-mass spectroscopy techniques have been used for the qualitative and quantitative evaluation of the phytochemicals present in the green gram seedlings. The antioxidant activity of AgNPs and GGSE was analyzed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. In vitro antibacterial activity was performed using the agar well diffusion method. Results: The presence of various secondary metabolites such as flavonoids, steroids, terpenoids, alkaloids, amino acids, polyphenol, glycoside, and protein was found in samples. The major chemical compounds of V. radiata were n-hexadecanoic acid, stigmasterol, caffeine, hexadecanoic acid, cholest-5-en-3-ol (3.beta.)-, and cyclopentane. The percentage of DPPH activity was enhanced on increasing the concentration of AgNPs. In vitro antibacterial effect of the diverse concentrations of AgNPs was investigated against each Gram-negative (Klebsiella aerogenes, and Escherichia coli) and Gram-positive (Bacillus substilis and Staphylococcus aureus) bacterial strains. Conclusion: The result suggests that biosynthesized AgNPs have good antibacterial and antioxidant activity and might be a potential for the bioactive components.

scholarly journals Volatile Cuticular Components Involved in Superficial Scald

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 605a-605
Author(s):  
S. Wee ◽  
R.M. Beaudry
Keyword(s):  
Gas Chromatography ◽  
Mass Spectroscopy ◽  
Malus Domestica ◽  
Apple Fruit ◽  
Superficial Scald ◽  
Conjugated Trienes ◽  
Gas Chromatography Mass Spectroscopy

Autoxidation products alpha-farnesene of have been implicated in superficial scald induction for apple (Malus domestica cv. Cortland Apple) fruit. We suspect the apple cuticle acts as a sink where α-farnesene can accumulate and eventually autoxidize into hydroperoxides, conjugated trienes, 6-methyl-5-hepten-2-one (ketone), and other compounds. These oxidized byproducts may diffuse back into the peel, thereby initiating the scald process. Cortland apples were stored at 0.8°C. Volatile cuticular components were analyzed at 2-week intervals by gas chromatography–mass spectroscopy. Only two scald-related volatiles were found, 6-methyl-5-hepten-2-one and α-farnesene. The identification of these compounds may allow the determination of cuticular involvement in superficial scald, as well as a possible correlation between the volatiles and apple scald development. α-farnesene concentrations initially increased and was followed by a decline, possibly due to its autoxidation.

scholarly journals Determination of phytocomponents of twenty-one varieties of smokeless tobacco using gas chromatography-mass spectroscopy (GC-MS)

2019 ◽  
Vol 72 ◽  
pp. 47-54 ◽  
Author(s):  
Hassan A. Alhazmi ◽  
Asaad Khalid ◽  
Shahnaz Sultana ◽  
Siddig I. Abdelwahab ◽  
Waquar Ahsan ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Mass Spectroscopy ◽  
Smokeless Tobacco ◽  
Gas Chromatography Mass Spectroscopy

Determination of Lightstruck Character in Beer by Gas Chromatography-Mass Spectroscopy

2000 ◽  
Vol 58 (4) ◽  
pp. 152-154 ◽  
Author(s):  
Susumu Masuda ◽  
Kaori Kikuchi ◽  
Koichi Harayama ◽  
Kazuhisa Sakai ◽  
Mitsuo Ikeda
Keyword(s):  
Gas Chromatography ◽  
Mass Spectroscopy ◽  
Gas Chromatography Mass Spectroscopy
Sign in / Sign up

Export Citation Format

Share Document