scholarly journals PRODUCTION AND CHARACTERIZATION OF EXOPOLYSACCHARIDE FROM MARINE MODERATELY HALOPHILIC BACTERIUM HALOMONAS SMYRNENSIS SVD III

2017 ◽  
Vol 9 (10) ◽  
pp. 146 ◽  
Author(s):  
Siddharth Deshmukh ◽  
Pradnya Kanekar ◽  
Rama Bhadekar
Keyword(s):  
Incubation Temperature ◽  
Environmental Parameters ◽  
Fold Increase ◽  
Halophilic Bacterium ◽  
Culture Conditions ◽  
Culture Broth ◽  
Inoculum Size ◽  
Moderately Halophilic Bacterium ◽  
Halomonas Smyrnensis

Objective: To study 1) Optimization of nutritional and environmental parameters to enhance the yield of EPS by Halomonas smyrnensis SVD III isolated from seawater, West Coast of Maharashtra, India and 2) Purification and characterization of the EPS produced.Methods: The isolate was grown in Sehgal and Gibbons (SG) medium broth supplemented with 3% glucose, at 37 °C, 120 rpm for 7 d. Optimization of different parameters was carried out with one factor at a time approach. EPS was isolated from cell-free supernatant of the culture broth by centrifugation and precipitation using chilled ethanol, after removal of proteins by trichloroacetic acid (TCA) treatment. Characterization of the purified EPS was carried out with respect to fourier-transform infrared (FTIR) spectrum, 1H nuclear magnetic resonance (NMR) spectrum and mass spectrometry (MS) analysis.Results: Two-fold increase in the yield of EPS (23 g/l) by the selected isolate was obtained by using culture conditions as 10% inoculum size having cell density of 107 cells/ml, pH 6, incubation temperature 45 °C, 3% carbohydrate, 0.5% yeast extract as nitrogen source, 20% salt concentration and 7 d of incubation period. Characterization of the purified EPS suggested the presence of dominated glycosidic linkages and heptasaccharide nature of the molecule. As the present strain is halophilic, 20% NaCl was found to be optimum.Conclusion: Optimization studies resulted in two-fold increase in the yield of EPS which is of heptasaccharide nature.

scholarly journals Production optimization using Plackett-Burman and Box-Behnken designs with partial characterization of amylase from marine actinomycetes

2021 ◽  
Author(s):  
Sarah I Bukhari ◽  
Mohamed H Al-Agamy ◽  
Mahmoud S Kelany ◽  
Mohammad R Al Hazani ◽  
Moaz M Hamed
Keyword(s):  
Molecular Weight ◽  
Specific Activity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Fold Increase ◽  
Fermentation Medium ◽  
Culture Conditions ◽  
Production Optimization ◽  
Activity Measurement

Abstract Amylase is an industrial enzyme that is used in the food and biofuel industries. We screened four actinomycetes strains for amylase biosynthesis. The Streptomyces rochei strain had a larger hydrolytic zone (24 mm) on starch agar plates, than the other isolates. Plackett-Burman’s experimental design was implemented to optimize the conditions for amylase production by the selected strains. Growth under optimized culture conditions led to 1.7, 9.8, 7.7, and 3.12 -fold increases for the isolates S. griseorubens, S. rochei, S. parvus, and Streptomyces sp., respectively, in the specific activity measurement in comparison with growth under primary conditions. When applying the Box-Behnken design on S. rochei using the most significant parameters starch, K2HPO4, pH, and temperature, there was a 12.22-fold increase in the specific activity measurement: 7.37 U/mg. The optimal fermentation medium formula was kept at 30.6°C for seven days. The amylase from S. rochei was partially purified, and its molecular weight was determined using Sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular weight was found to be 45, 43, and 53 kDa. Amylase was particularly active at pH 6 and 65°C. The purified enzyme was most active at 65°C and a pH of 6, thermal stability of 70°C for 40 min and salt concentration of 1 M with a Km and Vmax of 6.58 mg/ml and 21.93 mg/ml/min, respectively. The amylase improved by adding Cu + 2, Zn + 2, and Fe + 2 (152.21%, 207.24%, and 111.89%). Increased production of amylase enzyme by Streptomyces rochei KR108310 attracts the production of industrially significant products.

OPTIMIZATION OF LACCASE PRODUCTION BY CURVULARIA LUNATA USING MAIZE COB AS SUBSTRATE

2021 ◽  
Vol 4 (4) ◽  
pp. 460-468
Author(s):  
A. Bello ◽  
D. A. Machido ◽  
A. I. Mohammed-Dabo ◽  
S. A. Ado
Keyword(s):  
Laccase Activity ◽  
Incubation Temperature ◽  
Agricultural Research ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Laccase Production ◽  
Curvularia Lunata ◽  
Processing Unit ◽  
Crude Fibre ◽  
Maize Cob

Researchers are showing interest in laccase because it is able to degrade several lignocellulosic biomass. It is important to optimize laccase production to achieve higher yield at a lower cost using agricultural wastes. This study was aimed at optimizing the culture conditions for laccase production. Previously isolated and characterized laccase producing Curvularia lunata was collected from the laboratory of Microbiology department, ABU, Zaria. Maize cobs which were used as substrate were collected from Seed Processing Unit of Institute for Agricultural Research, Zaria and the proximate composition of the substrate was determined according to AOAC guideline. The optimum fermentation type for laccase production was determined after which the culture conditions for laccase production were optimized. Laccase activity was determination by guaiacol assay. The proximate compositions of the maize cob were found to be Moisture content (3.18%), Crude protein (2.50%), Crude fat (32.20%), Crude fibre (3.15), Ash content (1.88%) and Carbohydrate content (57.09%). Higher laccase activity was recorded under solid state fermentation compared to submerged fermentation. The optimum culture conditions were found to be inoculum size 3 x 5 mm, pH 5, incubation temperature 30 oC and incubation period of 6 days. The culture conditions for laccase production by Curvularia lunata using maize cob as substrate were optimized

scholarly journals Production and Partial Characterization of α-Amylase Enzyme from Marine Actinomycetes

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Mohamed H. Al-Agamy ◽  
Mohammad R. Alhuzani ◽  
Mahmoud S. Kelany ◽  
Moaz M. Hamed
Keyword(s):  
Specific Activity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Fold Increase ◽  
Culture Conditions ◽  
Activity Measurement ◽  
Terrestrial Environment ◽  
Box Behnken Design ◽  
Thermal Stability Of

Amylase producing actinobacteria were isolated and characterized from terrestrial environment. There are a limited number of reports investigating the marine environment; hence, in the present study, four marine enzymes were tested for their amylase production ability. On starch agar plates, the Streptomyces rochei strain showed a higher hydrolytic zone (24 mm) than the other isolates. Growth under optimized culture conditions using Plackett-Burman’s experimental design led to a 1.7, 9.8, 7.7, and 3.12-fold increase for the isolates S. griseorubens, S. rochei, S. parvus, and Streptomyces sp., respectively, in the specific activity measurement. When applying the Box-Behnken design on S. rochei using the most significant parameters (starch, K2HPO4, pH, and temperature), there was a 12.22-fold increase in the specific activity measurement 7.37 U/mg. The α-amylase was partially purified, and its molecular weight was determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. α-Amylase was particularly active at pH 6 and 65°C. The purified enzyme was most active at 65°C and pH 6, thermal stability of 70°C for 40 min, and salt concentration of 1 M with Km and Vmax of 6.58 mg/ml and 21.93 μmol/ml/min, respectively. The α-amylase was improved by adding Cu+2, Zn+2, and Fe+2 (152.21%, 207.24%, and 111.89%). Increased production of α-amylase enzyme by S. rochei KR108310 leads to production of significant industrial products.

Screening and characterization of the protease CP1 produced by the moderately halophilic bacterium Pseudoalteromonas sp. strain CP76

Extremophiles ◽  
2003 ◽  
Vol 7 (3) ◽  
pp. 221-228 ◽  
Author(s):  
Cristina Sánchez-Porro ◽  
Encarnación Mellado ◽  
Costanzo Bertoldo ◽  
Garo Antranikian ◽  
Antonio Ventosa
Keyword(s):  
Halophilic Bacterium ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium

scholarly journals Isolation and initial characterization of the tellurite reducing moderately halophilic bacterium, Salinicoccus sp. strain QW6

2008 ◽  
Vol 163 (4) ◽  
pp. 456-465 ◽  
Author(s):  
Mohammad Ali Amoozegar ◽  
Morahem Ashengroph ◽  
Feridon Malekzadeh ◽  
Mohamad Reza Razavi ◽  
Saied Naddaf ◽  
...  
Keyword(s):  
Halophilic Bacterium ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium ◽  
Initial Characterization
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