scholarly journals Genetic diversity analysis and population structure of some African and Asian Finger Millet (Eleusine coracana L.) accessions using Expressed Sequence Tags – Simple Sequence Repeat (EST-SSR) markers

2020 ◽  
Vol 5 (3) ◽  
pp. 643-646 ◽  
Author(s):  
Ernest C. Bwalya ◽  
Salem Marzougui ◽  
Esther Mwangi ◽  
Choi Wooseon ◽  
Myung-Chul Lee
Keyword(s):  
Genetic Diversity ◽  
Expressed Sequence Tags ◽  
Simple Sequence Repeat ◽  
Finger Millet ◽  
Eleusine Coracana ◽  
Diversity Analysis ◽  
Sequence Repeat ◽  
Genetic Diversity Analysis ◽  
Expressed Sequence ◽  
Simple Sequence

scholarly journals Development of Highly Polymorphic Expressed Sequence Tags-Simple Sequence Repeat Markers and Their Application in Analysis of Genetic Diversity of Chinese Bayberry (Morella rubra)

HortScience ◽  
2016 ◽  
Vol 51 (3) ◽  
pp. 227-231 ◽  
Author(s):  
Wenting Wang ◽  
Chao Feng ◽  
Zehuang Zhang ◽  
Liju Yan ◽  
Maomao Ding ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Expressed Sequence Tags ◽  
Simple Sequence Repeat ◽  
Mean Value ◽  
Sequence Repeat ◽  
Chinese Bayberry ◽  
Cultivated Populations ◽  
Expressed Sequence ◽  
Simple Sequence

Chinese bayberry (Morella rubra) is an economically important subtropical evergreen fruit crop native to China and other Asian countries. For facilitating cultivar discrimination and genetic diversity analysis, a total of 38 high-quality and highly polymorphic expressed sequence tags-simple sequence repeat (EST-SSR) markers, with little or no polymerase chain reaction (PCR) stutter bands, including 21 screened from those obtained previously and 17 newly developed markers, were developed. The average number of alleles (Na) per locus was 5.6, and polymorphism information content varied from 0.34 to 0.86, with a mean value of 0.57. With these markers, all 42 Chinese bayberry accessions analyzed were successfully discriminated and the phylogenetic relationship between accessions was revealed. The accessions can be separated into two groups with six subgroups. The grouping of four main cultivars in three subgroups and 12 white-fruited accessions, each with little or no anthocyanin accumulation in ripe fruit, into five subgroups suggested the preservation of broad diversity among cultivated populations. These EST-SSR markers and the findings obtained in this study can assist the discrimination of cultivars and lines and contribute to genetic and breeding studies in Chinese bayberry.

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