Features of morphotypic selection in populations of Serratula coronata L. cultivated in the conditions of the Moscow region

The article presents the results of morphotypic analysis of populations of perennial medicinal and fodder crops cultivated in the Moscow region (Serratula coronata L.). In the introduction, the botanical characteristics of the crowned sickle are given, the prospects and advantages of phytoecdysteroids obtained from raw materials are noted. The aim of the study is a comparative assessment of cultivated populations of S. coronata by morphotypic composition, determination of the age period for the selection of initial forms for breeding. The object of the study was the different geographical origin S. coronata three cultivated populations (Samarskaya, Belgorodskaya, Syktyvkarskaya from Rep. Komi). We have tested the application of methods for assessing varieties by uniformity, distinctness, stability for morphotypic selection in the population when creating improved, initial and breeding material. The article presents the results of the analysis of the morphotypic composition of populations by morphological characteristics and the productivity of raw materials according to long-term data (2013-2017, Moscow region, VILAR), the age period during which morphotypic selection should be carried out is established, morphotypes that are of the greatest value during selection are identified and described. The age period during which morphotypic selection should be carried out is established: 3-5 year, since the morphotype is fully formed by the 3rd year. It is established that plants in cultivated populations of Serratula coronata are represented by 2-3 morphotypes based on the height and leafiness of the generative shoot, the length of the stem leaves and the mass of the shoot. When selecting, the highest value is represented by tall morphotypes with medium-length leaves (~60-70% of them in each population), but morphotypes with medium leafiness should be selected among them (only ~41-51% of them in each population, only in the Syktyvkarskaya population – up to 69 %). The results of the study are relevant in the aspect of creating an improved, initial and breeding material of the Serratula coronata. Keywords: MEDICINAL PLANTS, SERRATULA CORONATA L., PHENOTYPIC VARIABILITY, MORPHOTYPE, POPULATION, PRODUCTIVITY, QUANTITATIVE CHARACTERISTICS, SELECTION

Validating South Sudan as a Center of Origin for Coffea arabica: Implications for Conservation and Coffee Crop Improvement

Cultivated Arabica coffee outside Ethiopia is plagued by low genetic diversity, compromising disease resistance, climate resiliency and sensory potential. Access to the wider genetic diversity of this species may circumvent some of these problems. In addition to Ethiopia, South Sudan has been postulated as a center of origin for Arabica coffee, but this has never been genetically confirmed. We used simple sequence repeat (SSR) markers to assess the genetic diversity of wild and cultivated populations of Arabica coffee from the Boma Plateau in South Sudan, against farmed accessions (of wild origin) from Ethiopia, Yemen, and global cultivars. Our results not only validate Boma Plateau as part of the natural distribution and as a center of origin for Arabica coffee but also indicate that wild populations in South Sudan are genetically distinct from Ethiopian Arabica. This newly identified genetic diversity within Arabica could have the potential for crop improvement through selection and use in breeding programs. Observations and analyses show that the extent and health of the wild population of Arabica in South Sudan have declined. Urgent action should be taken to conserve (in situ and ex situ) the unique, remaining genetic diversity of wild Arabica populations in South Sudan.

Elucidation of the Origin of the Monumental Olive Tree of Vouves in Crete, Greece

The olive tree of Vouves in Crete, is considered the oldest producing olive tree in the world with an estimated age exceeding 4000 years. In the present study, we sequenced two samples (from the bottom and the top of the tree) to elucidate the genetic relation of this ancient tree with other olive cvs as well as to gain some insights about its origin. Our results showed that both samples have different genetic origins, proving that this ancient tree has been grafted at least one time. On the basis of whole genome sequences the sample from the top of the Vouves tree showed relation of the same order than half-siblings to one accession corresponding to the present-day Greek cv ‘Mastoidis’. Nevertheless, in the framework of a microsatellite analysis it was found to cluster with the ‘Mastoidis’ samples. The Vouves rootstock (bottom sample) showed a clear grouping with the oleaster samples in a similar way to that of ‘Megaritiki’ Greek cv although it does not show any signal of introgression from them. The genomic analyses did not show a strong relation of this sample with the present-day Greek cvs analyzed in this study so it cannot be proved that it has been used as a source for cultivated olive tree populations represented by available genome sequences. Nevertheless, on the basis of microsatellite analyses, the Vouves rootstock showed affinity with two present-day Greek cvs, one “ancient” rootstock from continental Greece as well as monumental trees from Cyprus. The analysis of the impact of the variants in the gene space revealed an enrichment of genes associated to pathways related with carbohydrate and amino acid metabolism. This is in agreement with what has been found before in the sweep regions related with the process of domestication. The absence of oleaster gene flow, its old age and its variant profile, similar to other cultivated populations, makes it an excellent reference point for domestication studies.

Morphological Variation in Scarlet Plume (Euphorbia fulgens Karw ex Klotzsch, Euphorbiaceae), an Underutilized Ornamental Resource of Mexico with Global Importance

Morphological variation is useful in conservation and genetic improvement programs. Euphorbia fulgens, a range-restricted local endemic species of Mexico, is used locally during the altars in the festivities of different saints and is also cultivated as an ornamental plant mainly in Europe. Thus, in the present study, morphological variation was evaluated in wild populations and cultivated populations. Characterization of 90 individuals from three wild populations (the only ones recorded to date) was done by measuring 30 morphological traits both vegetative and reproductive. Thereafter, seeds were collected, and established under greenhouse conditions, and 39 morphometric variables were evaluated in adult plants. An analysis of variance (ANOVA) was done for wild and cultivated groups independently, and when significant differences were found, Tukey’s comparison of means was applied (p < 0.05). To identify the traits responsible for the differences between wild and cultivated groups, a linear discriminant analysis (LDA) was conducted. Morphological variation was found among wild populations, and this variation decreased in cultivated populations, mainly in reproductive structures. The LDA separated the wild populations from the cultivated groups, according to inflorescence length, petiole length/blade length ratio, and leaf roundness. The variables that determined the separation of individuals between wild and cultivated populations were cyme number, foliar Feret diameter, and inflorescence length, variables that can be important for breeding strategies and artificial selection.

Genetic Differentiation for Gene Diversity among Pearl Millet (Pennisetum glaucum (L.) R. Br.) Landraces As Revealed by SSR Markers

Pearl millet (Pennisetum glaucum) is one the most important crops in Burkina Faso. Knowledge of the genetic parameters of different pearl millet landraces grown in Burkina Faso is of great importance for improvement and breeding programs of pearl millet. In this study, 20 pairs of SSR markers were used to compare and analyze the genetic diversity of 86 landrace populations. Results show a total of 67 alleles detected and each locus varies between 2 and 6 with an average of 4 alleles per marker. The probability to having two different alleles per locus was 0.61. The values of genetic diversity parameters of pearl millet populations from the northern region of the country were higher than those from the others regions. The analysis indicates that, over time, alleles are lost and new alleles are added. Analysis of molecular variance showed genetic variation was primarily a function of differences in cultivated populations. Most pairwise FST comparisons between provenances were at a statistically significant level. Our study will provide a scientific basis for the effective management and utilization of germplasm resources of pearl millet populations.

After Habitat Changed, Evaluation For Genetic Diversity And Secondary Metabolic Changes of Cultivated And Wild Populations of Medicinal Plants

Background: With the increasing use of traditional herbal medicine, the issue of the genetic diversity of medicinal plants has received considerable critical attention. A high degree of genetic diversity is the basis for maintaining the stability and long-term survival of the population, so the changes in genetic diversity and genetic structure of cultivated medicinal plants caused by habitat changes can not be ignored; Meanwhile, the difference of secondary metabolism of medicinal plants caused by habitat change is also deserving of attention simultaneously. And It is also worth pondering whether the changes between them are consistent. Results: In this study, the wild and cultivated populations of Polygonatum odoratum, Dioscorea nipponica and Acanthopanax sessiliflorus were selected as the research objects. and the genetic structure and HPLC fingerprint between wild and cultivated populations were compared and analyzed by using Simple Sequence Repeats (SSR) marker and HPLC . The results demonstrated that the wild and cultivated populations of the three medicinal plants maintained higher genetic diversity, however, the genetic structure of wild populations of P.odoratum and D.nipponica is more similar. In addition, there was a great genetic differentiation between P.odoratum and D.nipponica populations. There were significant differences in HPLC fingerprints among different populations, in which the secondary metabolites of wild populations were more complex, nevertheless, there was less difference in HPLC fingerprints between wild and cultivated populations of P.odoratum and D.nipponica. Spearman correlation analysis implied that environmental factors (including soil environmental factors, rainfall, temperature) had significant effects on the secondary metabolites of the three medicinal plants, whereas, soil environmental factors had less effect on the genetic structure of the three medicinal plants. Conclusion: In conclusion, during the cultivation years, environmental factors only have a significant effect on the secondary metabolism of the three medicinal plants, their populations still maintain higher genetic diversity and stable genetic structure, what’s more, the secondary metabolites of the same medicinal plants with stable and similar genetic structure may still be different from each other when the habitat is changed. It indicated that there is no obvious consistency between them during the cultivation years.

Antimicrobial Potential and Phytochemical Profile of Wild and Cultivated Populations of Thyme (Thymus sp.) Growing in Western Romania

The purpose of this study was to analyze the chemical composition and antimicrobial activity of some thymus populations collected from five different locations in Western Romania. The chemical compositions of the essential oils (EOs) were studied through GC–MS, and the biological activities were evaluated using the microdilution method. The EO yield ranged between 0.44% and 0.81%. Overall, 60 chemical compounds were identified belonging to three chemotypes: thymol (three populations), geraniol (one population) and carvacrol (one population). Thymus vulgaris L. is distinguished by a high content of thymol, while species of spontaneous flora (Th. odoratissimus and Th. pulegioides) contain, in addition to thymol, appreciable amounts of carvacrol and geraniol. The antimicrobial activity of each the five oils was tested on Staphylococcus aureus (ATCC 25923), Streptococcus pyogenes (ATCC 19615), Esherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Shigella flexneri (ATCC 12022), Salmonella typhimurium (ATCC 14028), Haemophilus influenzae type B (ATCC 10211), Candida albicans (ATCC 10231) and Candida parapsilopsis (ATCC 22019). The EOs showed biological activity on Gram-positive/Gram-negative/fungal pathogens, the most sensitive strains proving to be S. pyogenes, S. flexneri, S. typhimurium and C. parapsilopsis with an MIC starting at 2 µL EO/100 µL. The species sensitive to the action of Thymus sp. from culture or spontaneous flora are generally the same, but it should be noted that T. odoratissimus has a positive inhibition rate higher than other investigated EOs, regardless of the administered oil concentration. To date, there is no research work presenting the chemical and antimicrobial profiling of T. odoratissimus and the correlations between the antimicrobial potential and chemical composition of wild and cultivated populations of thyme (Thymus sp.) growing in Western Romania.

Evolution under domestication of correlated characters in populations of Stenocereus stellatus (Pfeiff.) Riccob., under different forms of management in central Mexico: genetic diversity, damage, and defense mechanisms

Across the process of domestication, human selection produces changes in target attributes as well as changes that are not necessarily desired by humans due to pleiotropic or linked genes. In this paper we addressed, correlated changes between genetic diversity, damage level, defense mechanisms (resistance and tolerance), and fitness due to the domestication process of Stenocereus pruinosus (Otto ex Pfeiff.) Buxbaum and Stenocereus stellatus (Pfeiff.) Riccobono, an endemic columnar cactus of south-central Mexico. One hundred eighty individuals of S. stellatus from wild, in situ managed, and cultivated populations of Valle de Tehuacán and Mixteca Baja, Puebla, were sampled, and attributes including damage level, defense mechanisms and fitness (number of fruits) were measured. The DNA of 176 individuals was extracted to amplify and analyze five microsatellites in order to estimate genetic diversity and structure. As expected, cultivated populations showed a significantly higher damage level, as well as lower resistance and genetic diversity. Depending on the form of management, correlations between genetic diversity and the rest of the attributes exhibited different patterns. In wild populations, genetic diversity was positively correlated with damage and negatively with resistance; in situ managed populations exhibited the opposite pattern, and in cultivated populations, no correlations were found between these attributes. We propose a hypothetic model of human selection to explain the variation in these correlations. No differences in genetic diversity and tolerance were detected between regions; however, the populations of Valle de Tehuacán exhibited more damage and more resistance. In both regions, populations showed a positive correlation between fitness and resistance and a negative correlation between damage and resistance, suggesting the existence of a defense mechanism to ensure fitness. Also, non-regional differentiation suggests an eventual gene flow due to pollinators, human movement of branches, or a common ancestry before the domestication process.

Genome-Wide SNPs Provide Insights on the Cryptic Genetic Structure and Signatures of Climate Adaption in Amorphophallus albus Germplasms

Domesticated species represent unique systems in which the evolutionary genomic consequences of intensive selective breeding and adaptation can be thoroughly investigated. Amorphophallus albus occurs naturally and is in cultivation throughout the downstream region of the Jinshajiang River in Southwest China. This species is characterised by high konjac glucomannan content, and has been cultivated in China for nearly 2,000 years. To study genetic differentiation and local adaption of A. albus, we sampled 13 distinct local cultivated populations of this species. Restriction site-associated DNA sequencing was conducted with 87 samples, resulting in 24,225 SNPs. The population structure analyses suggest two main genetic groups: one in the relatively upstream region, and one downstream. We found evidence of additional sub-structure within the upstream group, demonstrating the statistical power of genomic SNPs in discovering subtle genetic structure. The environmental and geographic factors were all identified as significant in shaping the genetic differentiation of this species. Notably, the proportion of environmental factors was larger than geographic factors in influencing the population genetic patterns of A. albus. We also discovered loci that were associated with local adaptation. These findings will help us understand the genetic differentiation of this newly domesticated species, thereby informing future breeding programs of A. albus.