CircRNA hsa_circ_0004771 promotes esophageal squamous cell cancer progression via miR-339-5p/CDC25A axis

Epigenomics ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 587-603 ◽  
Author(s):  
Enmin Huang ◽  
Junhui Fu ◽  
Qiuyan Yu ◽  
Peixin Xie ◽  
Zhongxian Yang ◽  
...  
Keyword(s):  
Cancer Progression ◽  
Squamous Cell ◽  
Squamous Cell Cancer ◽  
Cell Cancer ◽  
Tumor Burden ◽  
Esophageal Squamous Cell Cancer ◽  
Regulatory Relationship ◽  
Pair Plasma

Aim: The role of circRNAs in esophageal squamous cell cancer (ESCC) remains unclear. Materials & methods: Here we profiled six pair plasma circRNA in ESCC based on RNA sequencing, and then verified the elevation of hsa_circ_0004771 in 20 cancer tissues and 105 pair case–control plasma samples by quantitative reverse transcriptase PCR. Results: The upregulation of hsa_circ_0004771 was correlated with heavier tumor burden and poor prognosis, knockdown of it inhibited the ESCC cells proliferation both in vitro and in vivo. Mechanistically, hsa_circ_0004771 positively regulated CDC25A by acting as a molecular sponge of miR-339-5p and rescue assay confirmed this regulatory relationship. Conclusion: These results suggested that hsa_circ_0004771 can serve as a general less-invasive biomarker and may provide diagnostic and prognostic value in carcinoma.

Circular RNA circ‐Foxo3 inhibits esophageal squamous cell cancer progression via the miR‐23a/PTEN axis

2019 ◽  
Vol 121 (3) ◽  
pp. 2595-2605 ◽  
Author(s):  
Yao Xing ◽  
Wen‐Juan Zha ◽  
Xiao‐Min Li ◽  
Hao Li ◽  
Fei Gao ◽  
...  
Keyword(s):  
Cancer Progression ◽  
Squamous Cell ◽  
Squamous Cell Cancer ◽  
Cell Cancer ◽  
Circular Rna ◽  
Esophageal Squamous Cell Cancer

scholarly journals MicroRNA-214-3p Inhibits the Stem-Like Properties of Lung Squamous Cell Cancer by Targeting YAP1

2020 ◽  
Author(s):  
Tingting Lu ◽  
Ying Yang ◽  
Ziming Li ◽  
Shun Lu
Keyword(s):  
Squamous Cell ◽  
Squamous Cell Cancer ◽  
Cell Cancer ◽  
Underlying Mechanism ◽  
Cell Counting ◽  
Hippo Signaling ◽  
Kaplan Meier ◽  
Luciferase Activity Assay

Abstract Background: Emerging evidence reveals that microRNAs (miRNAs) play a crucial role in tumor progression, but the underlying mechanism of microRNAs in lung squamous cell cancer (LSCC) remains unclear. Method : Western-blotting and quantitative real-time PCR (q-PCR) were carried out to detect mRNA and protein expression. Cell proliferation was evaluated by Cell Counting Kit-8 (CCK-8), colony-forming assay or sphere-forming assay, respectively. Results: MiR-214-3p was markedly de-regulated in LSCC tissues and was inversely related to the level of Yes-associated protein1 (YAP1), which is the core transcription regulator of the Hippo signaling pathway. Kaplan-Meier survival curves illustrated that patients with high miR-214-3p expression demonstrated more favorable clinical outcomes. MiR-214-3p overexpression (OE) repressed proliferation and cancer stem-like cells (CSCs) properties in vitro and in vivo xenograft mouse model. Mechanistically, luciferase activity assay revealed that miR-214-3p directly targets YAP1 by specifically binding on the 3’ UTR of YAP1. Conclusion: MiR-214-3p plays a pivotal role in CSCs properties by targeting YAP1, which provides a potential treatment strategy for LSCC patients.

scholarly journals MicroRNA-214-3p Inhibits the Stem-Like Properties of Lung Squamous Cell Cancer by Targeting YAP1

2020 ◽  
Author(s):  
Tingting Lu ◽  
Ying Yang ◽  
Ziming Li ◽  
Shun Lu
Keyword(s):  
Squamous Cell ◽  
Squamous Cell Cancer ◽  
Cell Cancer ◽  
Underlying Mechanism ◽  
Cell Counting ◽  
Hippo Signaling ◽  
Kaplan Meier ◽  
Luciferase Activity Assay

Abstract Background: Emerging evidence reveals that microRNAs (miRNAs) play a crucial role in tumor progression, but the underlying mechanism of microRNAs in lung squamous cell cancer (LSCC) remains unclear. Method : Western-blotting and quantitative real-time PCR (q-PCR) were carried out to detect mRNA and protein expression. Cell proliferation was evaluated by Cell Counting Kit-8 (CCK-8), colony-forming assay or sphere-forming assay, respectively. Results: MiR-214-3p was markedly de-regulated in LSCC tissues and was inversely related to the level of Yes-associated protein1 (YAP1), which is the core transcription regulator of the Hippo signaling pathway. Kaplan-Meier survival curves illustrated that patients with high miR-214-3p expression demonstrated more favorable clinical outcomes. MiR-214-3p overexpression (OE) repressed proliferation and cancer stem-like cells (CSCs) properties in vitro and in vivo xenograft mouse model. Mechanistically, luciferase activity assay revealed that miR-214-3p directly targets YAP1 by specifically binding on the 3’ UTR of YAP1. Conclusion: MiR-214-3p plays a pivotal role in CSCs properties by targeting YAP1, which provides a potential treatment strategy for LSCC patients.

Efficacy of transfection rates on head and neck squamous cell cancer by a novel adenovirus: An in vivo and in vitro study

Head & Neck ◽  
2002 ◽  
Vol 24 (12) ◽  
pp. 1038-1046 ◽  
Author(s):  
Elise S. Lalonde ◽  
Gregory Beyer ◽  
Paul L. Friedlander ◽  
Jay K. Kolls
Keyword(s):  
Head And Neck ◽  
Squamous Cell ◽  
Squamous Cell Cancer ◽  
Cell Cancer ◽  
In Vitro Study ◽  
Vitro Study ◽  
Neck Squamous Cell Cancer

scholarly journals Expression of p14ARF, p15INK4b, p16INK4a and skp2 increases during esophageal squamous cell cancer progression

2012 ◽  
Vol 3 (6) ◽  
pp. 1026-1032 ◽  
Author(s):  
PENG BAI ◽  
XUE XIAO ◽  
JUAN ZOU ◽  
LIN CUI ◽  
TRI M. BUI NGUYEN ◽  
...  
Keyword(s):  
Cancer Progression ◽  
Squamous Cell ◽  
Squamous Cell Cancer ◽  
Cell Cancer ◽  
Esophageal Squamous Cell Cancer

scholarly journals MicroRNA-214-3p Inhibits the Stem-Like Properties of Lung Squamous Cell Cancer by Targeting YAP1

2020 ◽  
Author(s):  
Tingting Lu ◽  
Ying Yang ◽  
Ziming Li ◽  
Shun Lu
Keyword(s):  
Squamous Cell ◽  
Squamous Cell Cancer ◽  
Cell Cancer ◽  
Underlying Mechanism ◽  
Cell Counting ◽  
Hippo Signaling ◽  
Direct Target Gene ◽  
Luciferase Activity Assay

Abstract Background: Emerging evidence reveals that microRNAs (miRNAs) play a crucial role in tumor progression, but the underlying mechanism in lung squamous cell cancer (LSCC) remains unclear.Method : Western-blotting, Quantitative Real-time PCR (q-PCR) or was carried out to detect protein expression. Cell proliferation was evaluated by Cell Counting Kit-8 (CCK-8), Colony-forming assay or sphere-forming assay, respectively.Results: MiR-214-3p was found to be markedly de-regulated in LSCC tissues and was inversely related to the level of Yes-associated protein1 (YAP1), which is the core transcription regulator of the Hippo signaling. The Kaplan-Meier survival curves illustrated that patients with high miR-214 expression demonstrated more favorable clinical outcomes. The miR-214-3p overexpression repressed proliferation and cancer stem-like cells (CSCs) properties in vitro and in vivo xenograft mouse model. Mechanistically, luciferase activity assay revealed YAP1 was a direct target gene of miR-214-3p.Conclusion: Our findings illustrated that miR-214-3p plays a pivotal role in CSCs properties by targeting YAP1, which provides a potential treatment strategy for LSCC patients.

Effects of rofecoxib and leptin on esophageal squamous cell cancer in vitro

2003 ◽  
Vol 124 (4) ◽  
pp. A795 ◽  
Author(s):  
Linda C. Vona-Davis ◽  
Ponnandai Somasundar ◽  
Dale Riggs ◽  
Barbara Jackson ◽  
David W. McFadden
Keyword(s):  
Squamous Cell ◽  
Squamous Cell Cancer ◽  
Cell Cancer ◽  
Esophageal Squamous Cell Cancer
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